LOCUS       Exported                1069 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    S1PR2-Tango
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg ggctcacttt acagtgaata cctgaatccc aacaaggtcc
      301 aagaacacta caattatacc aaggaaacac tggagacaca ggaaacgact tcccgacagg
      361 tggcctccgc attcattgtg attctctgct gtgcaatcgt ggtggagaat ctgcttgtgc
      421 tgatcgcagt agctaggaat agcaaatttc atagcgctat gtatctcttc cttggaaatc
      481 tggccgcttc tgaccttctt gcgggtgttg ccttcgtggc aaatacactg ttgtccgggt
      541 ccgttacatt gcggctcacc cccgtgcagt ggtttgcccg cgagggctcc gcctttatca
      601 ctctgtccgc ttccgtcttc agcctcctgg cgatcgccat tgagagacat gtggcaatcg
      661 cgaaagtgaa actgtacggg agtgacaaga gctgcagaat gctgctgctg atcggcgcaa
      721 gctggttgat cagtctggtt ttgggcggcc tgcccatcct cgggtggaac tgtctcggtc
      781 atctcgaagc atgtagcact gtgttgccac tgtacgcaaa acattacgtc ctgtgcgttg
      841 ttactatctt tagcattatt ctgcttgcaa tcgtggcact gtacgtaagg atatactgtg
      901 tggtcagaag ttctcacgct gatatggcag cacctcagac cctcgcactc ctgaaaacag
      961 tcaccattgt gctgggagtg ttcatagtgt gttggctgcc cgcgttcagc atattgctct
     1021 tggactacgc ctgtcccgtg cattcctgcc ccatcttgta taaggccat
//
