LOCUS       Exported                 806 bp ds-DNA     linear   SYN 22-MAY-2021
DEFINITION  Beta-2-adrenergic receptor tagged with mCerulean on carboxyl 
            terminus.
ACCESSION   .
VERSION     .
KEYWORDS    Beta-2-adrenergic receptor-mCerulean
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH
  TITLE     Live cell imaging of Gs and the beta2-adrenergic receptor 
            demonstrates that both alphas and beta1gamma7 internalize upon 
            stimulation and exhibit similar trafficking patterns that differ 
            from that of the beta2-adrenergic receptor.
  JOURNAL   J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
  PUBMED    15297467
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(124..375)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(166..187)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     515..534
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     774..795
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
       61 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct
      121 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      181 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta
      241 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      301 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc
      361 gttcttctgc ttgtcggcgg tgatatagac gttgtcgctg atggcgttgt actccagctt
      421 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      481 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      541 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      601 cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggtcaggg tggtcacgag
      661 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
      721 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
      781 gtccagctcg accaggatgg gcacca
//
