LOCUS       Exported                 980 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  Vector for cotranslational expression of tagRFP-Bxb1.
ACCESSION   .
VERSION     .
KEYWORDS    pCAG-tagRFP-NLS-HA-Bxb1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             67..87
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             88..114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa tcgacccgcc
       61 accatgccaa aaaagaaaag aaaagtgtat ccctatgatg tccccgatta tgccggttca
      121 agagccctgg tcgtgattag actgagccga gtgacagacg ccaccacaag tcccgagaga
      181 cagctggaat catgccagca gctctgtgct cagcggggtt gggatgtggt cggcgtggca
      241 gaggatctgg acgtgagcgg ggccgtcgat ccattcgaca gaaagaggag gcccaacctg
      301 gcaagatggc tcgctttcga ggaacagccc tttgatgtga tcgtcgccta cagagtggac
      361 cggctgaccc gctcaattcg acatctccag cagctggtgc attgggctga ggaccacaag
      421 aaactggtgg tcagcgcaac agaagcccac ttcgatacta ccacaccttt tgccgctgtg
      481 gtcatcgcac tgatgggcac tgtggcccag atggagctcg aagctatcaa ggagcgaaac
      541 aggagcgcag cccatttcaa tattagggcc ggtaaataca gaggctccct gcccccttgg
      601 ggatatctcc ctaccagggt ggatggggag tggagactgg tgccagaccc cgtccagaga
      661 gagcggattc tggaagtgta ccacagagtg gtcgataacc acgaaccact ccatctggtg
      721 gcacacgacc tgaatagacg cggcgtgctc tctccaaagg attattttgc tcagctgcag
      781 ggaagagagc cacagggaag agaatggagt gctactgcac tgaagagatc tatgatcagt
      841 gaggctatgc tgggttacgc aacactcaat ggcaaaactg tccgggacga tgacggagcc
      901 cctctggtga gggctgagcc tattctcacc agagagcagc tcgaagctct gcgggcagaa
      961 ctggtcaaga ctagtcgcgc
//
