LOCUS       Exported                1111 bp ds-DNA     linear   SYN 08-JUN-2021
DEFINITION  Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into
            a MIN-tagged locus.
ACCESSION   .
VERSION     .
KEYWORDS    attB-GFP-Dnmt3b6-Poly(A)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      72..81
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             161..172
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagagcg gcctgaggag cagagcccag gcgagcaaca
       61 gcgccgtgga cgccaccatg ggcgatcgca tgaagggaga cagcagacat ctgaatgaag
      121 aagagggtgc cagcgggtat gaggagtgca ttatcgttaa tgggaacttc agtgaccagt
      181 cctcagacac gaaggatgct ccctcacccc cagtcttgga ggcaatctgc acagagccag
      241 tctgcacacc agagaccaga ggccgcaggt caagctcccg gctgtctaag agggaggtct
      301 ccagccttct gaattacacg caggacatga caggagatgg agacagagat gatgaagtag
      361 atgatgggaa tggctctgat attctaatgc caaagctcac ccgtgagacc aaggacacca
      421 ggacgcgctc tgaaagcccg gctgtccgaa cccgacatag caatgggacc tccagcttgg
      481 agaggcaaag agcctccccc agaatcaccc gaggtcggca gggccgccac catgtgcagg
      541 agtaccctgt ggagtttccg gctaccaggt ctcggagacg tcgagcatcg tcttcagcaa
      601 gcacgccatg gtcatcccct gccagcgtcg acttcatgga agaagtgaca cctaagagcg
      661 tcagtacccc atcagttgac ttgagccagg atggagatca ggagggtatg gataccacac
      721 aggtggatgc agagagcaga gatggagaca gcacagagta tcaggatgat aaagagtttg
      781 gaataggtga cctcgtgtgg ggaaagatca agggcttctc ctggtggcct gccatggtgg
      841 tgtcctggaa agccacctcc aagcgacagg ccatgcccgg aatgcgctgg gtacagtggt
      901 ttggtgatgg caagttttct gagatctctg ctgacaaact ggtggctctg gggctgttca
      961 gccagcactt taatctggct accttcaata agctggtttc ttataggaag gccatgtacc
     1021 acactctgga gaaagccagg gttcgagctg gcaagacctt ctccagcagt cctggagagt
     1081 cactggagga ccagctgaag cccatgctgg a
//
