LOCUS Exported 11958 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants. ACCESSION . VERSION . KEYWORDS pMpGWB115 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11958) AUTHORS Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, Shikanai T, Kohchi T TITLE Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. JOURNAL PLoS One. 2015 Sep 25;10(9):e0138876. doi: 10.1371/journal.pone.0138876. eCollection 2015. PUBMED 26406247 REFERENCE 2 (bases 1 to 11958) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11958 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 54..78 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 266..288 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 280..297 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 281..297 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 323..447 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 484..514 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 568..1227 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(634..653) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 1569..1874 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 1753..1772 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(1915..2039) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 2050..2766 /codon_start=1 /product="enhanced variant of YFP (Heikal et al., 2001)" /label=Citrine /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2095..2116) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2356..2375) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2703..2724 /label=EGFP-C /note="EGFP, forward primer" CDS 2773..2793 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 2818..3070 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(3104..3120) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3104..3120) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3117..3139) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3128..3144 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3152..3182) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3197..3218 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." polyA_signal 3303..3477 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(3518..4543) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" promoter complement(4610..5285) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" misc_feature 5480..5504 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 6025..6816 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 7062..7650 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7551..7570 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7804..7821 /label=L4440 /note="L4440 vector, forward primer" misc_feature 7836..7976 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(7902..7921) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8062..8084 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 8320..8514 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(8580..9653) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(10082..10711) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" ORIGIN 1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc 61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga 181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa 361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg 421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca 481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg 541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa 1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc 1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg 1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac 1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg 1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga 1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg 1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca 1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg 1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg 1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt 1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt 1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt 1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt 2041 gataacagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 2101 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 2161 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 2221 tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac 2281 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 2341 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 2401 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 2461 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 2521 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 2581 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 2641 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 2701 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 2761 tacaagttgc agcctaagaa gaagagaaag gttggaggat agagctcgaa tttccccgat 2821 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg 2881 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg 2941 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg 3001 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg 3061 ttactagatc gggaattggt tccggaacca attcgtaatc atggtcatag ctgtttcctg 3121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 3181 aagcctgggg tgcctaatga gtgagctaac tcacattagg ctgaattagg cgcgcctatt 3241 tctgaattca acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg 3301 gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag 3361 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac 3421 cctataggaa ccctaattcc cttatctggg aactactcac acattattat ggagaaactc 3481 gagcttgtcg atcgacagat cccggtcggc atctactcta tttctttgcc ctcggacgag 3541 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg 3601 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg 3661 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat 3721 agagttggtc aagaccaatg cggagcatat acgcccggag tcgtggcgat cctgcaagct 3781 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc 3841 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa 3901 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca 3961 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg 4021 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag 4081 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg 4141 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata gcctccgcga 4201 ccggttgtag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg 4261 aacggcggga gatgcaatag gtcaggctct cgctaaactc cccaatgtca agcacttccg 4321 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat 4381 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac 4441 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca 4501 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg 4561 atctgcgaaa gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc 4621 tctccaaatg aaatgaactt ccttatatag aggaaggtct tgcgaaggat agtgggattg 4681 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt 4741 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact 4801 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt 4861 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc 4921 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga 4981 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc 5041 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg 5101 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt 5161 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt 5221 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag 5281 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag 5341 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccga attcagaaat 5401 aaattcagcc taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa 5461 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc 5521 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg 5581 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat 5641 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc 5701 atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag 5761 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac 5821 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct 5881 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt 5941 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt 6001 cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa 6061 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat 6121 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt 6181 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa 6241 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg 6301 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg 6361 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct 6421 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa 6481 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta 6541 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc 6601 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca 6661 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt 6721 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac 6781 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag 6841 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc 6901 tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc 6961 ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc 7021 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 7081 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 7141 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 7201 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 7261 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 7321 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 7381 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 7441 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 7501 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 7561 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 7621 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 7681 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 7741 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 7801 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg 7861 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 7921 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 7981 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 8041 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 8101 cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg 8161 gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg 8221 gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc 8281 gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt 8341 ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt 8401 atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac 8461 gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag 8521 agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat 8581 taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg 8641 ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc 8701 ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc 8761 gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga 8821 aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg 8881 ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc 8941 ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat 9001 accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg 9061 tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg 9121 cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc 9181 ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg 9241 taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga 9301 agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc 9361 acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa 9421 tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt 9481 tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct 9541 tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca 9601 tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt 9661 cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc 9721 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt 9781 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg 9841 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct 9901 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc 9961 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg 10021 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa 10081 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc 10141 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc 10201 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct 10261 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc 10321 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt 10381 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag 10441 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct 10501 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc 10561 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg 10621 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc 10681 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac 10741 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca 10801 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag 10861 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg 10921 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa 10981 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg 11041 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg 11101 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc 11161 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg 11221 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc 11281 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca 11341 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc 11401 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg 11461 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt 11521 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg 11581 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg 11641 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg 11701 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga 11761 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg 11821 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg 11881 ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat 11941 ggacgaacgg ataaacct //