LOCUS       Exported               16904 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene 
            (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr 
            II.
ACCESSION   .
VERSION     .
KEYWORDS    pSR13
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16904)
  AUTHORS   Ruijtenberg S, van den Heuvel S
  TITLE     G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit 
            during Muscle Differentiation.
  JOURNAL   Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. 
            Epub 2015 Jul 2.
  PUBMED    26144318
REFERENCE   2  (bases 1 to 16904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16904
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1559..1579
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3007..3031
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3038..3054
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3038..3054
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3993..4026
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     CDS             4055..4075
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    11442..11475
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     CDS             11504..11524
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(11537..11839,11893..11967,12019..12138,12190..12405)
                     /codon_start=1
                     /product="monomeric enhanced GFP, optimized with synthetic 
                     introns for expression in C. elegans"
                     /label=mEGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNVKRDHMVLKE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(12637..12654)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12637..12653)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             14594..14605
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(14751..14775)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(16113..16133)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(16337..16356)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16456..16478
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(16516..16534)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        16602..16706
                     /gene="bla"
                     /label=AmpR promoter
     CDS             16707..663
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca
     1561 actatgtata ataaagttgt gtttttgaac acggcgatat gtatctgtag atcatcccca
     1621 attttcaaca atgaatctaa atactgaact ttttgtgtaa tctttagtgc agaaaaatga
     1681 ccaaaatcag cgattttccg attataccca aaatatctca gctcaatgag cacctataaa
     1741 aatgtaaaaa taaagaaagt agtcgaacca tattttgtaa aggctaaaaa actgaataaa
     1801 tttttatcca tccagcgcgg cgttacaatc cgtggcccat gtcacgcacg gctttcaaag
     1861 aaaaaaaaac agtctgtttg cgcgacatcg atctggcaaa aatcctaaaa acgactatgc
     1921 gtggctcact ttatattaat atgaaaaata caaacttggt ggtgcaggtg ctgcagaggc
     1981 catcgatgaa gaagtggagg ccttgttgtc gatctacaaa attgtatggt tgtttgagat
     2041 tttccttgaa acgctctcaa tcttggatat gatataaatt ctggataaga tatgatggat
     2101 atgatctatg tttgatagag tgcatttgaa aggataacgg ccctgcagac atttttgaaa
     2161 aaataacaaa catttaattt tctcactcgt ttaggctatt cccctatttt tgatattcct
     2221 tcgcacatat gaaaactact ttttttcgaa actgttaact ccagaattta taaatctata
     2281 gcccttactt gattatttat tatcatggtt actcacctca tgtctctttc tcttttcaag
     2341 aagtcttcgt tcatatctgt tgtctctttc ctccttttga atctctgctt tgctcttcgc
     2401 cattgtttcc tgaaaataat gtaacttgaa ttgtgtaata tactttttta atttgaattt
     2461 ggcttgtaac gcggaatcac tacgtgcggg atcatttctt actagaaaac ccagaaaatg
     2521 ccatatttca cttatctcgg ggtcatttct aattagaaaa gctacaaaaa ccatttctaa
     2581 ttacggtgac tttagaactg gtcgaaatag ctaattagaa atgggttttt gctaattaga
     2641 ggtgactcta tgttatctga gtaagcctcg tttgtatgta aactcacagt actaataaat
     2701 gcaagacacc cgggtttgtc tagatatgaa ataattgaaa tatcaattct gacagacaat
     2761 aatggtaatc ttgataagga gttccacgcc caggagaaca cgttagtttt cttgtttttg
     2821 attgcgtgcg ttattttgga gaaaaactcg attttttaca aaataatttt ttgaaaggaa
     2881 cactgttcaa taagttttgt cttttttctc agttgtgata cggtttttta ttcttttttg
     2941 tagttataca gaagaccgtt acgaaacaga ctgatatcga aacaaacact gatcttactt
     3001 gcacttacca ctttgtacaa gaaagctggg tcacgggcag gaaacagcta tgaccatgat
     3061 tacgccaagc tacgtaatac gactcactag tgggcagatc ttcgaatgca tcgcgcgcac
     3121 cgtacgtctc gaggaattcc tgcagctgca gttcaatcgg tttttccttg cttgcgccca
     3181 attctgatgg ttcttccatt tctaaatttt agtttaataa aaaaatttta aaataatacg
     3241 agttttgaga ctaactttca ataaaaacca aaaaaaattg cttcaaaaaa gagttcgcgc
     3301 cgatttacga taaacacagc gcgctacgac actccgaaaa ccgcgccgca ccattacatt
     3361 ctccgcccca ccgcacggtt tgcggaaggc gatgaaattg ctgggcctaa aattttaacc
     3421 ctttttttcc cgtttttcaa ctgttttccc gattttttcg atattttttt caggttttta
     3481 gttagaaaag tatataaaaa ttgcattatt tgtagttttc gctcgtaatt tgtgatagaa
     3541 aacggccaat ttgcctagat ttgtgattct accaagtgga atttttggga aaatatcaat
     3601 attccacgat ttttattcaa atcataacgt ttcgaaatta tcagcttaaa taaaactaaa
     3661 ataaataata aaatactaaa aaccctgaat ttctcggttc tcggttcagc ttgagttcgt
     3721 tctatacacg ttatcgattt ttgtcatttc gtttaatcat gggttttgat tcattttaaa
     3781 tatgaaattt gatttattcc tcgtttcgaa gttggtttgt cttgtccggc cggtaaacaa
     3841 atgttattaa aaaaataata aatttcgaaa tttttcgtaa tgttccgcaa tcgtgttttt
     3901 ccgtttatct cgtcttccct tctttcaaat atactattta ttttctcaat tcacatattt
     3961 tcagccagcc ccgctcaaca agtggaataa aaataacttc gtatagtata ccttatacga
     4021 agttattcta gaggatccag aaaaaatgac tgctccaaag aagaagcgta aggtaccggt
     4081 agaaaaaatg tccaagggag aggaggacaa catggccatc atcaaggagt tcatgcgttt
     4141 caaggtccac atggagggat cagtcaacgg acacgagttc gagatcgagg gagagggaga
     4201 gggacgtcca tacgagggaa cccaaaccgc caagctcaag gtaagtttaa acatatatat
     4261 actaactaac cctgattatt taaattttca ggtcaccaag ggaggaccac tcccattcgc
     4321 ctgggacatc ctctccccac aattcatgta cggatcaaag gcctacgtca agcacccagc
     4381 cgacatccca gactacctca agctctcctt cccagaggga ttcaagtggg agcgtgtcat
     4441 gaacttcgag gacggaggag tcgtcaccgt cacccaagac tcctccctcc aagacggaga
     4501 gttcatctac aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     4561 tcaggtcaag ctccgtggaa ccaacttccc atccgacgga ccagtcatgc aaaagaagac
     4621 catgggatgg gaggcctcct ccgagcgtat gtacccagag gacggagccc tcaagggaga
     4681 gatcaagcaa cgtctcaagc tcaaggacgg aggacactac gacgccgagg tcaagaccac
     4741 ctacaaggcc aagaagccag tccaactccc aggtaagttt aaacatgatt ttactaacta
     4801 actaatctga tttaaatttt caggagccta caacgtcaac atcaagctcg acatcacctc
     4861 ccacaacgag gactacacca tcgtcgagca atacgagcgt gccgagggac gtcactccac
     4921 cggaggaatg gacgagctct acaagtaaga attcgctagc cggccataca agtaatccgg
     4981 atgatcgacg ccaacgtcgt tgaattttca aattttaaat actgaatatt tgtttttttt
     5041 cctattattt atttattctc tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat
     5101 ccaaatctaa acattttttt ttctctttcc gtctcccaat tcgtattccg ctcctctcat
     5161 ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg acgtgggggg
     5221 aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg acacacaata
     5281 tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat aaaaatatat
     5341 agaggaggaa ggaaaactag ttctagagcg gccgcccatt atcatcattg tgacgctttt
     5401 cattgaacaa caaaacaaga catttttcaa attaattatc gttttttccg ttgtattttc
     5461 aacttttttg atgtggttaa atacccccct caatgctcta tttttgctct ttttcagctc
     5521 gaaaactgga atttctactg aaatttacgt aatttttgcc attttttcgc tgaaatttgc
     5581 tagaaatcgc taaaaaatgc gaatttttcg gaaaaaaagc ccgatttcct caattttcat
     5641 caaaaaaagt tcaaaataac tacaaactac agctttttgg gcgctgctaa aactacaaac
     5701 tacaagtttt gggcgctgct aattcaaatt ataggcgctg ctaaaactac aaactacagc
     5761 tttttgggcg ctgctaattc aaattatagg cgctgcgaaa actacaaact acaagttttg
     5821 ggcgctgcta attcaatttt aggcgctgct aaaactacaa actacaagtt ttgggcgctg
     5881 ctaattcaaa ttttaggcgc tgctaaaact acaaactaca agttttgggc gctgctaatt
     5941 cgattttggg cgctgctaaa actacaaact acagcttttt gggcgctgct atgcttgaat
     6001 ttgtcaattt tttcgattta ttcgaatttt ttcgcttaaa atcaattttt aatcaaattt
     6061 tcttcaactt tcctgaattc taactacaaa ctacaagatt ttagctcgat ttttctcaaa
     6121 aatgcttcaa atttcggaaa ttttccggga tttttccctg gaaaaatcga aaattttcaa
     6181 atttcctaca attcccgctc tattttagcg ctgcaaaatc ccaattaccc caagaaaatc
     6241 gataattccc ccaccgtcct acaggtcccc ccgtattcca ttttctgagc agatgttcat
     6301 cccgcgttct ctctctccct ctccacgagg cctcctttct ctgcgctctc tctcgtttct
     6361 ccacactatt tctctcgttt tctctgtttt ctctggcgtc tctcactacg cgcgagctcc
     6421 ctcacactgc cgccttgttt gaatctacgc gggcctcgat atttcgatat tggaccaatt
     6481 tcgaccaatt ttctcgcagt tttttgttaa aagtctcttt ttaaggttat ttagtcataa
     6541 tttcattgaa aatttcgaaa gaaattccaa gaaaatacga gatttttgca gtttccacag
     6601 ttttctgtta aaattcttga ttttacagac atttttccta gaaattttgc cgtttttctg
     6661 ctcacttcag tgaaaaaaat cgattttttc gcaattttct gcccataaat atgctttttc
     6721 ctcgctgaaa accctgaaaa tcttttcaat tccccgctaa atttcagcaa aatttcgtcg
     6781 aaaagtggaa tgaagaaggg agcgtcgaag cggagacgcg acgacgatga cggtgacgtt
     6841 tcgatgaccg gcgacgacga tgatagcaga agtggtgccg ctgtgaaggt tgaagtgccg
     6901 aaaggtagcg aaatctgtgt ggaattttcg gcgaaaatgc ggaaaaatag ctctgaaaaa
     6961 cgcatttttt agtgattttc tccgacaaaa tcgaaaaaaa tcgagcaaaa tccgtgaaaa
     7021 tctggctccg ggcagcccga ataccctcaa aaagtgcaat ttcagcgtca gagctcaagt
     7081 ttttgcaata actgactgga aaactttgat tttctgggaa atttgcataa tttttccatt
     7141 ttttccggaa ttgagagttg aaaaacgcaa aaaattggtt ttttacactc gaaaatgctt
     7201 ttttcaacac attttttacg caaaagtgaa cttttttttt ttcaagtttc attcgaaaaa
     7261 gaccgaaatt tgaattttgg gcgctgctaa gctaaatttt agcgaaaatt ggtcaaaatt
     7321 cgaacatttc cagagcgcat tttcaaaatt tctactcatt ttttggaaaa aaaatcggaa
     7381 atttaaattt tagccaaaaa tggaattttt cagcgctttt tcttcagatt ttcttcgaaa
     7441 tttctgaaaa aatcgcattt ttatctgatt tttccagaaa aaaaccaatt tttcggtcaa
     7501 aattgcattt caggaccaaa aaaaaccaga tttttccaaa tttccttcct aaaatttttt
     7561 tgggcgctgc taaagctaca aactacaagt tttgggcgct gctaaactac aaactacaag
     7621 atttgggcgc tgctaattcg attttgggcg ctgctaaact acaaactaca agatttgggc
     7681 gctgctaata caattttggg cgctgctaaa ctacaaacta caagatttgg gcgctgctaa
     7741 ttcaattttg ggcgctgcta aactacaaac tacaagtttt gggcgctgct aattcgattt
     7801 tgggcgctgc taaactacaa actacaagat ttgggcgctg ctaatacaat tttgggcgct
     7861 gctaaactac aaactacaag atttgggcgc tgctaattca attttgggcg ctgctaaact
     7921 acaaactaca agttttgggc gctgctaatt caattttggg cgctgctaat tcaattttgg
     7981 gcgctgctga ccgattttgg gcgctgctag actacaaact acaagatttg ggcgctgcta
     8041 attcaatttt gggcgctgct aacactacaa actacaggtt ttttgcgctg ctaatccaat
     8101 tttgggcgct gctaaactac aaactacaag atttgggcgc tgctaattca attttgggcg
     8161 ctgctaacac tataaactac aggttttttg cgctgctaat ccaattttgg gcgctgctaa
     8221 actacaaact acaagatttg ggcgctgcta attcaatttt gggcgctgct aacactacaa
     8281 actacaggtt ttttgcgctg ctaatccaat tttgggcgct gctaaactac aaactacaag
     8341 atttgggcgc tgctaattca attttgggcg ctgctagact acaaactaca agatttgggc
     8401 gctgctaatt caattttggg cgctgctaaa ctacaaacta caagttttgg gcgctgctaa
     8461 tccaatttcc cctcaatttc caaccaattc tcttttgcag gaaaggaaaa agacgccgaa
     8521 ttcagcgcac caaaaggcca aaagctcact gatctcgacg aagaaggtgt ccaatcaaca
     8581 aaagaagctc cacaactcgc cgaaggaaac gtcattgagc agacccacta cattgtagtc
     8641 ccctcatacg ccggctggtt cgactataac gcaattcatc aaatcgagaa acgcgcaatg
     8701 cctgaatttt tcaacggaaa gaataagagc aagacaccgg acgtctacgt tgcctaccgt
     8761 aattttatgg tcgacacgta ccgtctgaat ccatttgaat acgtgtctgc gacggcttgt
     8821 cgccggaatt tggccggtga tgtgtgctca attgtgcggc ttcactcgtt tctcgagcaa
     8881 tggggcctgc tgaattatca agttgattcg gatgctcggc cggctcctgt tgctccgcca
     8941 ccaacaagcc attttatggt tttagctgat acaccgactg gcattcagcc aatgaatcca
     9001 cctggaaagg agtctgctgg agcatctggg gagccaccaa aagaggagat taagactgag
     9061 attgagtcca tttcgacgcc cggcctgaaa attgatcagt atcagaagca ggccatcgcg
     9121 atgagaacaa aaggagcccc accaggccgt gattggaccg aacaggagac gtgtttgctg
     9181 ttggaagcat tggaaatgtt taaggatgac tggaataagg tttgcgacca cgtcggcact
     9241 cgaactcaac acgaatgtgt tctaaaattc cttcaactcc caattcaaga cccatatcta
     9301 actgaaaatc tgtcgtcgga taaagctgaa gcagctcccg gcggagcagc caaagaagta
     9361 ctcggaccac ttgcattcca gcctgttcca ttttctcaat ctggaaatcc agtaatgtca
     9421 actgtggctt ttctggcatc agttgtggat ccacaagtgg ccgctgcagc tacaaaagct
     9481 gcaatggaag aatttggaaa attgaaagaa gaaattccgc cgcttgtagc tgaagctcat
     9541 gagaaaaatg ttgcggcaat ggccgaaaag actgggcaag ttgatggagc tgttgggctt
     9601 acgaaaagtg gcctaaagcc tgctgaagag gccgccggag attccgatga aaaaatggat
     9661 accaatacca acgacgacgt tccatcaacg accgaagcga aatcagccat cgataaagga
     9721 gttcaggctg cggctgcctc atgcctagcc gccgccgccg taaaagccaa gcatctggct
     9781 cagattgagg aacgccgtat caaaagcctc gtcgcacagc ttgtcgagac tcaaatgaag
     9841 aagcttgaga tgaagcttcg tcattttgac gagctcgagc agattatgga taaggagcgg
     9901 gaatcgttgg aatatcaacg gcatcagttg attcttgaac gtcaggcttt tcatatggat
     9961 cagcttaaat atctcgagaa tcgggctaaa catgaagctc attctcggat gacaagctct
    10021 ggagctcttc cggctgggct tccacctgga tttgaggtta ccggcccgcc acagcctact
    10081 cctcaggtgc agattagtgc tcaggaggct gcgattccag agaagatgga tacttcggaa
    10141 gcagcgactg ctgctcggcc accgtccaca ccacaggctc cgcaagctcc ccccgttcag
    10201 gccgctccgg cgccggttca ggctcctcag gctccacagg ctccaccaca agcctaccaa
    10261 ggctatggag ggcctggagg gccccctcaa caagcctacc gctacccacc acaacaaggc
    10321 caacaatact caccataccc accaccccaa caacaacaac aacatcaggc tcagcaggcc
    10381 cagtctcagg ctcactatgg cccaccgggt ggaggacaag gcccaccacc accaccacaa
    10441 ggccagcaat actatggagg gccgccgcca ccgggccagc cgtacgggcc gccaggaggc
    10501 tatccgccac agcagcagag gccaccatat caggctcaac cgtatccggg cccgccgccg
    10561 cctcagcagc agaggggata tggttatcca ccaccaccgc agccaggaca cccataccag
    10621 cagccatatg gtcaaatgcc accaccacca cacggacaat atcaaccaca gcagcagcag
    10681 ggaggtccaa tgggaccacc cgggggacat catgaaggtg gagacggtgg agattcacaa
    10741 cagaaacagg aataatttat ggaaattttg aattttttct gatttttttc tgattttttt
    10801 tgcgcatttt tttgttaaaa ttctcggatt tttttttttg gaaatttttt gtgaaaaatc
    10861 gctgaatttt tctgattttt ttgcgaattt tttggtatat tttggacatt tttcagattt
    10921 tttttctcaa aatttttaga aaattcgctg attttttccc aaaatttatc cgaattttct
    10981 aaaattttct ggattttttc aaaaattccc tattttttca gattttttgc gaatttttga
    11041 gcattttttg gaaattttgt cctcaaaatt ttccattttt tctcgaattt tttttacaaa
    11101 atttttcact gaattttccc aattttaatc ctatttcctc cgatcgcatt ttttaaaatg
    11161 ttttaccacc cccttatata catatttacc cccattctgt ttcccccgta aaaaaaccac
    11221 caaaatgatc tttttttctc tctctgaaat atatatatat gtaacacccc tccgaactcc
    11281 acgctgctgg tatctccttt tttttcatgg ttttttttaa cgattttaat tttaatttat
    11341 tcgtatcttg tgctatttaa caccccacca acacatatta ctctctctct ttcttttttt
    11401 ctatttatat gatgaaaacg cacacttttt cgagcggccg cataacttcg tatagtatac
    11461 cttatacgaa gttattctag aggatccaga aaaaatgact gctccaaaga agaagcgtaa
    11521 ggtaccggta gaaaaaatgt ccaagggaga ggagctcttc accggagtcg tcccaatcct
    11581 cgtcgagctc gacggagacg tcaacggaca caagttctcc gtctcaggag agggagaggg
    11641 agacgccacc tacggaaagc tcaccctcaa gttcatctgc accaccggaa agctcccagt
    11701 cccatggcca accctcgtca ccaccttcac ttacggagtc caatgcttct cccgttaccc
    11761 agaccacatg aagcgtcacg acttcttcaa gtccgccatg ccagagggat acgtccaaga
    11821 gcgtaccatc ttcttcaagg taagtttaaa cattaattaa tactaactaa ccctgattat
    11881 ttaaattttc aggacgacgg aaactacaag acccgtgccg aggtcaagtt cgagggagac
    11941 accctcgtca accgtatcga gctcaaggta agtttaaaca gttcggtact aactaaccat
    12001 acatatttaa attttcaggg aatcgacttc aaggaggacg gaaacatcct cggacacaag
    12061 ctcgaataca actacaactc ccacaacgtc tacatcatgg ccgacaagca aaagaacgga
    12121 atcaaggtca acttcaaggt aagtttaaac atgattttac taactaacta atctgattta
    12181 aattttcaga tccgtcacaa catcgaggac ggatctgtcc aactcgccga ccactaccaa
    12241 caaaacaccc caatcggaga cggaccagtc ctcctcccag acaaccacta cctctccacc
    12301 caatccgccc tctccaagga cccaaacgtg aagcgtgacc acatggtcct caaggagttc
    12361 gtcaccgctg ccggaatcac ccacggaatg gacgagctct acaagtagga attcgctagc
    12421 cggccataca agtaatccgg atgatcgacg ccaacgtcgt tgaattttca aattttaaat
    12481 actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct
    12541 ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc gtctcccaat
    12601 tcgtattccg caggccttaa gtgagtcgta ttacggactg gccgtcgttt tacacccgtg
    12661 cagttgaaat tgaaaatgag ttaaagttgg agtttttatt gaaaacagat ttccgtgtga
    12721 ttagtgtttt tagcgagtgt gacaggacag cgaaaaaata tagaaacaag gggggaactg
    12781 aaaagcttag gaatgcattg aacatgagaa ggggaagggg aaggaacaaa ctagacagga
    12841 attattggaa tttaatcaca tttggagttt tttttctatt cgacagaata attatccaga
    12901 acatttttgt attaaatatt tatgcatcat atgagtagtc ggctttgttg tgcatgacga
    12961 gtttgttatc gacgaaatag aagctgtcag aacgagtctc gtttggattg ttgatcatgt
    13021 cgtccactga aaaagagatt agtcttttga attgtacttt ttagaataat gactcactga
    13081 gttgttgaga gagttgaggg aactcataga tatgttcaca gttgtttcgt gaattcggaa
    13141 tacagaatcc gaattcaaag tcaaaacact tcagaaggcg atctttgaag aagtgacgtt
    13201 cgatcattcg gaaatgatgg attgggatgt ctcctacttt gaattccaca gttgctccga
    13261 ccgttttgag tttcaaaaag tttggagcaa atctataacg cacgtatctt gccgactcct
    13321 gtggcgactc atcattctcc tgatcgttct ccggtttggc gatctcgaaa agcacttgct
    13381 cagtgtccag atcacggatt tggaacttgg tgaactcgat gttatagatg ttcgcagatg
    13441 gggagcataa gaatcctaaa tttatgtttt aaactgaaat ccaaagggag caagatacct
    13501 tgagtgattc ccggaagtgc taaaacgtcg ttcggagtga tttgagcttt cttcgcaagc
    13561 tccgattccg ttgtgattcc ttgttcggtg cttggtggtg gccgtggcat ctggaaatat
    13621 ggaaaagttc aacaaaaaga aaagagaaaa gaatgaaatc ggatatcaag agttagttga
    13681 gcggtttctc tagttttctg agtctcacct gcgacgggaa ggtcgccgag ccgggtggaa
    13741 tcgatcgttg ttgctcggct ttcatatcgg tttggttgga agcggctgaa aacggaaaga
    13801 agtggaagaa ggaaaagagt gtggtgtgac aggaaaatgg taattagagg gtgccaaata
    13861 accagctata ttttgttttt ttttgaaaac atttttaaaa agaaaaatac gataatgata
    13921 tcagatggat ttccggaaaa ctggtatgaa aaatttcaac ctttttgagt acatgtaatc
    13981 aaaatacact ttgtaaatta tcatttttat tgaaactcca ccatttttct atttataacg
    14041 ctaataattt gaaaaagaaa cctattgcga accgcggggt gaatcccaaa aacgaatgcg
    14101 ttttggtgga gtgattgatt cgaatcgaag aagaaaaaga agaagacgtg gaatagagag
    14161 ctcactctta accgagcagc acacaccgac agaaaaaaaa atgaaatgaa tgagggtctt
    14221 cttcttcttc ttcttcgaat gattgacaga aatgggaaaa agaggaagat tgagaaggga
    14281 aaaaggaagg agaaaagaag cagaagaaga cgtcagagag gagaggaacg agcggaaaag
    14341 cagcgggcgc aagtcataga agtagcagag ctggggagaa gaagacacta tccaagaaag
    14401 gaatgacgag agagtatgca aaggggtata gggtgcagac agaataggaa cagaataaca
    14461 gatgatgagc caagaagagt tgaaaagggc gatgaatttg tcatgtaact taatttgggt
    14521 caatttgagc atgatgaatt gaaatcatcc cttgttggga gttaataacc ggtttgttat
    14581 cagaaaccct gtaatagaag ggcgccctaa ctttgagcca attcatcccg gtttctgtca
    14641 aatatatcaa aaagtggtca actgacaaat tgtttttgat attataataa acattttatc
    14701 cgttaacaat tttcgaatac tttttacaag gacttggata aattggctca agcctgcttt
    14761 tttgtacaaa cttgacgaag tgagtttgct accatcatgg aatcaggagg acatttcgac
    14821 aatgtcatat aatgtttgaa aaatgatttt aacaagggtc tcattcgaga taagcaatgc
    14881 tctgagcaag tggctcgtcg tcttcaagaa tctcttgttg atattcattt ggatcctgtt
    14941 ttgcacgaag catgtgccat ggatattcag agtttttgca aaaaagttcc gccaggtcaa
    15001 acaatctttt taatttttat atgatacttc gggtgtattt ttcttctttt tcctgttcat
    15061 tttcgctttt ttctcacttt tttgtaacaa aatctctcac ttttctgtaa gtcttaacaa
    15121 aagttcaatt ggctcgatca agtaaaattt cggccgtatg aacgttttca aaggttttca
    15181 ttttttttta agttcattca caatatcatg aatctctcgt cctttggttt ccggtaagta
    15241 gcgaaataat ataagcatac ttataaatga aggaattgaa tatagaataa ggaaagcata
    15301 actaccaata acggaatata gaggtaatac tgtaaatgta ctgataacca ccattacagt
    15361 attcattgaa tatgccacag attgagcaat actacgatgt ttttgaggta ccagttcaga
    15421 ggatataaac cacgaaattg gaccaactcc agttctgaaa tttcattatt cgattaaatc
    15481 aaaatatatt tgaaaaacct cacccataag tataaccgta gatgatcaat gcaactaggc
    15541 atccgtattt catcgggtca atcagaatac gtatttttgc gaagaatacg aatgcaatta
    15601 gagctaaatt attgagaaat gtgaagagaa ggagaagctt gcgtcgacca actctgtaaa
    15661 cttattcata caattttgtc ttcaaatttt gaacgtttaa aactatctta ttcaatgagt
    15721 tagcgggttt ttatagtgag ttagttcacg attatctaat aaaactatgt ccaaacatcc
    15781 taactgcgac cgtattctca ctaactcgtc ttaaaaacgt tgagattatt ttaaaccctt
    15841 cttcagtaca actacagtac tgatgcacaa atacaataat aataatacat tcagacatac
    15901 tctgaacaat gtaacttgtt tttgttatac tgtggtaaca tttattgaaa tgaaatataa
    15961 ccttactttt caatcacaat tccaccagta attgttccag aaacataagc taaacccatt
    16021 gcagttgtac tccattcagc aatttctgac tcaacatttg cattttctag aaagaaagta
    16081 gaggataaaa gaagagccca taatccgaca agcaactttt ctatacaaag ttgatagctt
    16141 ggcggatccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
    16201 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
    16261 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
    16321 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
    16381 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
    16441 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
    16501 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
    16561 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    16621 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    16681 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    16741 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    16801 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    16861 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatg
//
