LOCUS Exported 2932 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMLLKA-J72021 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2932) TITLE J.C. Anderson Lab Plasmids REFERENCE 2 (bases 1 to 2932) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2932 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 44..76 /codon_start=1 /product="HSV (herpes simplex virus) epitope tag" /label=HSV tag /translation="QPELAPEDPED" primer_bind complement(106..123) /label=L4440 /note="L4440 vector, forward primer" protein_bind complement(185..209) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 256..360 /gene="bla" /label=AmpR promoter CDS 361..1221 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(579..598) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" primer_bind complement(1502..1521) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1513..2328 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1586..1605) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2411..2767 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" protein_bind 2829..2853 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gatctcgaac acccatatca atattaaagg gaaggtaagc atgcagccgg agctcgcacc 61 agaagacccg gaagactgcg gatcctaact cgctcctcag gcttcctcgc tcactgactc 121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatcaa 181 ttcgacccag ctttcttgta caaagtggtt gatcggatct cttagacgtc aggtggcact 241 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 421 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg cagaggatca gttgggtgca 481 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 541 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 601 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 661 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 721 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 781 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 841 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 901 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 961 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 1021 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 1081 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 1141 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 1201 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 1261 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatcggatc 1321 tctgaagact ctcgagctgc aggactcaca gctcctcgga tctgctgatc cttcaactca 1381 gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt 1441 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat 1501 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctccaggcc gcgattaaat 1561 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 1621 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 1681 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 1741 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 1801 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca 1861 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 1921 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 1981 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 2041 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat 2101 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 2161 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 2221 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 2281 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 2341 aattggttgt aacactggca gagcattacg ctgacttgac gggaggatct agcagttcaa 2401 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt 2461 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc 2521 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa 2581 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata 2641 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac 2701 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg 2761 gctgacatgg gaattagcca tgggcccgtg cgaatcacgg atctcccgat caagaattgc 2821 cattatcaac aagtttgtac aaaaaagcag gctccgaatt ggtatcacga ggcagaattt 2881 cagataaaaa aaatccttag ctttcgctaa ggatctgaag tggaattcat ga //