LOCUS Exported 1134 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines. ACCESSION . VERSION . KEYWORDS attB-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1134) AUTHORS Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut P, Herzog F, Leonhardt H, Bultmann S TITLE A modular open platform for systematic functional studies under physiological conditions. JOURNAL Nucleic Acids Res. 2015 May 24. pii: gkv550. PUBMED 26007658 REFERENCE 2 (bases 1 to 1134) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1134 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(69..116) /label=EM7 promoter /note="synthetic bacterial promoter " protein_bind complement(122..155) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(559..579) /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS complement(592..636) /codon_start=1 /product="affinity and epitope tag derived from pancreatic ribonuclease A" /label=S-Tag /translation="KETAAAKFERQHMDS" CDS complement(676..699) /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" /label=HRV 3C site /translation="LEVLFQGP" ORIGIN 1 gcggccggag aacctgcgtg caatccatct tgttcaatgg ccgatcccat ggtttagttc 61 ctcaccttgt cgtattatac tatgccgata tactatgccg atgattaatt gtcaacagaa 121 tataacttcg tataatgtat gctatacgaa gttatcgagc ggccgcagta gcatagggtt 181 tgcagacagc tctccttata tagaccggat cccaccgtac acacttacct ggacgtagcc 241 ctcgggcatg gcgctcttga agaaatcgtg ctgcttcatg tggtcggggt atctgctgaa 301 gcactgcacg ccgtaggtca gggtggtcac cagggtgggc cagggcacgg gcagcttgcc 361 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcgtcgcctt cgccctcgcc 421 ggacacgctg aacttgtggc cgttcacgtc gccgtccagc tccaccagga tgggcaccac 481 gccggtgaac agttcctcgc ccttggacac ggatgatcct gacgacggag accgccgtcg 541 tcgacaagcc ggtccgcctc cctgaaagta caggttctcg cctcctcctc cgctgtccat 601 gtgctgccgc tcgaatttag cagcagctgt ttctttgaac ttggcggcgc tgccagaggg 661 gccagagctg ctcagggggc cctggaacag cacttccagg ctgctgctgc cgctggggcc 721 gctgctcttg tacagctcgt ccatgcccag ggtgattccg gcggctgtca caaattcttt 781 cagcaccatg tggtcccgct tctcgttggg gtccttgctg agagcgctct gggtgctcag 841 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg 901 gtcggccagc tgcacgctgc cgtcctcgat gttgtgccgg atcttgaagt tgaccttgat 961 gccgttcttc tgcttgtcgg ccataatgta cacgttgtgg ctgttgtagt tgtattccag 1021 cttgtggccc aggatgttgc cgtcctcttt gaagtcgatg cccttcagct cgatcctgtt 1081 caccagtgtg tcgccctcga acttcacctc ggccctggtc ttgtagttgc cgtc //