LOCUS Exported 2929 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMLLKA-J72027 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2929) TITLE J.C. Anderson Lab Plasmids REFERENCE 2 (bases 1 to 2929) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2929 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 44..76 /codon_start=1 /product="epitope tag from vesicular stomatitis virus G protein" /label=VSV-G tag /translation="YTDIEMNRLGK" primer_bind complement(103..120) /label=L4440 /note="L4440 vector, forward primer" protein_bind complement(182..206) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 253..357 /gene="bla" /label=AmpR promoter CDS 358..1218 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(576..595) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" primer_bind complement(1499..1518) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1510..2325 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1583..1602) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2408..2764 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" protein_bind 2826..2850 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gatctcaata gaacattaga tcagaacgcg aaagggaaca atgtacaccg atatagagat 61 gaacaggctg ggaaagggat cctaactcgc tcctcaggct tcctcgctca ctgactcgct 121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatcaattc 181 gacccagctt tcttgtacaa agtggttgat cggatctctt agacgtcagg tggcactttt 241 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 301 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 361 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 421 tttgctcacc cagaaacgct ggtgaaagta aaagatgcag aggatcagtt gggtgcacga 481 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 541 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 601 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 661 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 721 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 781 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 841 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 901 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 961 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 1021 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 1081 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 1141 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 1201 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 1261 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tcggatctct 1321 gaagactctc gagctgcagg actcacagct cctcggatct gctgatcctt caactcagca 1381 aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca 1441 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca 1501 aggggtgtta tgagccatat tcaacgggaa acgtcttgct ccaggccgcg attaaattcc 1561 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt 1621 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc 1681 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa 1741 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc 1801 accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt 1861 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt 1921 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat 1981 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa 2041 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt 2101 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt 2161 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt 2221 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat 2281 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat 2341 tggttgtaac actggcagag cattacgctg acttgacggg aggatctagc agttcaacct 2401 gttgatagta cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttaacgtact 2461 aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc tcatggctaa 2521 cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata aaattaatat 2581 aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa aagaatatat 2641 aaggctttta aagcttttaa ggtttaacgg ttgtggacaa caagccaggg atgtaacgca 2701 ctgagaagcc cttagagcct ctcaaagcaa ttttgagtga cacaggaaca cttaacggct 2761 gacatgggaa ttagccatgg gcccgtgcga atcacggatc tcccgatcaa gaattgccat 2821 tatcaacaag tttgtacaaa aaagcaggct ccgaattggt atcacgaggc agaatttcag 2881 ataaaaaaaa tccttagctt tcgctaagga tctgaagtgg aattcatga //