LOCUS Exported 823 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system. ACCESSION . VERSION . KEYWORDS FH-Tet2-pEF SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 823) AUTHORS Ko M, Huang Y, Jankowska AM, Pape UJ, Tahiliani M, Bandukwala HS, An J, Lamperti ED, Koh KP, Ganetzky R, Liu XS, Aravind L, Agarwal S, Maciejewski JP, Rao A TITLE Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2. JOURNAL Nature. 2010 Dec 9;468(7325):839-43. doi: 10.1038/nature09586. PUBMED 21057493 REFERENCE 2 (bases 1 to 823) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..823 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 26..45 /label=T7 /note="T7 promoter, forward primer" promoter 26..44 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 142..165 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 178..204 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" ORIGIN 1 gtgtcgtgag gaattagctt ggtactaata cgactcacta tagggagacc caagctggct 61 aggtaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt cgcccttaga 121 tcttccgctc gatgaaccat ggactacaag gacgacgatg acaagctcga tggaggatac 181 ccctacgacg tgcccgacta cgccggagga ctcgaggaac aggacagaac cacccatgct 241 gagggcacca gactgagtcc attcctgata gcaccacctt ctcccatcag ccatacagaa 301 cctctggctg tcaaactcca gaatggaagc ccgttagcag agagacctca tccagaagta 361 aatggagaca ccaagtggca atcttcccaa agctgttatg gaatatccca catgaaaggc 421 agccagagca gtcatgagag tccacatgaa gacagagggt attccaggtg cttacagaat 481 ggagggataa aacgcacagt cagtgaacca tctctctctg ggctccatcc aaacaagata 541 ttgaaactag accaaaaagc taagggagaa agcaatatct tcgaggaaag ccaagaaaga 601 aaccacggta aaagcagccg tcagccaaat gtctccggac taagtgataa tggagagccc 661 gtgacctcga ccacccagga aagttcaggt gcagatgctt tcccaacacg gaactacaat 721 ggagttgaaa ttcaggttct caacgagcag gaaggggaaa aaggcaggag cgttacatta 781 cttaaaaaca agattgtgct aatgcctaat ggtgctacag ttt //