LOCUS Exported 13737 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants. ACCESSION . VERSION . KEYWORDS pMpGWB328 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13737) AUTHORS Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, Shikanai T, Kohchi T TITLE Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. JOURNAL PLoS One. 2015 Sep 25;10(9):e0138876. doi: 10.1371/journal.pone.0138876. eCollection 2015. PUBMED 26406247 REFERENCE 2 (bases 1 to 13737) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13737 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 54..78 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 266..288 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 280..297 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 281..297 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 812..1157 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" protein_bind 1179..1303 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1340..1370 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1424..2083 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1490..1509) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 2425..2730 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 2609..2628 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(2771..2895) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" terminator 3644..3896 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(3930..3946) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3930..3946) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3943..3965) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3954..3970 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3978..4008) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4023..4044 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." polyA_signal 4129..4304 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" promoter complement(6388..7064) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" misc_feature 7259..7283 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 7804..8595 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 8841..9429 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9330..9349 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9583..9600 /label=L4440 /note="L4440 vector, forward primer" misc_feature 9615..9755 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(9681..9700) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9841..9863 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 10099..10293 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(10359..11432) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(11861..12490) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" ORIGIN 1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc 61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga 181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc 361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata 421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga 481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag 541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta 601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa 661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca 721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca 781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg 841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa 901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg 961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag 1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa 1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat 1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg 1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac 1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc 1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt 1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac 1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca 1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa 1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct 1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga 1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg 1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg 1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc 1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt 1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc 1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa 2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt 2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata 2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca 2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg 2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag 2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg 2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc 2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg 2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg 2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg 2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac 2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct 2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt 2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt 2881 cttgtacaaa gtggttgata acagcgctgg aggctccggt gggagcatga gcgagctgat 2941 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa 3001 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt 3061 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg 3121 cagcagaacc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc 3181 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac 3241 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa 3301 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga 3361 gatgctgtac cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt 3421 gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa 3481 gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc 3541 cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc 3601 tagcaaactg gggcacaagt gagcttagag ctcgaatttc cccgatcgtt caaacatttg 3661 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt 3721 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag 3781 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat 3841 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga 3901 attggttccg gaaccaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 3961 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 4021 taatgagtga gctaactcac attaggctga attaggcgcg cctatttctg aattcaacac 4081 attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt 4141 agtactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac 4201 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct 4261 aaattccctt atctgggaac tactcacaca ttattatgga gaaactcgaa gttagtatat 4321 agtccttcca tcaccctctg tgataatctc cttaaaactt gctcccccag gaatcatagg 4381 cagaacgtgc tcctggtggg gaactatcac atccagtaaa taaggaccgg gagaatctag 4441 catagtttga attgctgctc ttagttcatc cttcttcacc acacgcgcat ttggcacctt 4501 gaaaccgtct gcaactgtac agaagtcggg gtagatctgc tcctcgtgat ctggatcacc 4561 taagtaagta tgtgctctgt tggccttgta gaatctatcc tccaattgta cgaccattcc 4621 tagatgttgg ttgtttaaga tcatgatctt cacgggtaac ttctccacaa aaattgtagc 4681 caactcctgt acgttcatca caaagctacc atcgccatcg atgtcgatca caggaatatc 4741 tggttgagtt gcggcagccc ccaaagcaga aggaagtccg aatcccatgg ctccaagacc 4801 tccagatgtc aaccaacgcc gcggttcgtt gtacttgaac cactgggctg cccacatttg 4861 atgttggcca acaccagtag atatgatggc atttccacca gtgagttcgt acagcgtctg 4921 tatggcagat tgaggagcaa tggcatcctc gaatacggga aacttcattg gccatttctg 4981 cttttgttct tctagttctg ccacccagtt ggagaagtcg aaagtcctct tttgactctt 5041 caagagggca ttgatttcac ttagcgcagg tttcacatca gcacacatgg acacatgggg 5101 ctgtttgttc tttccaattt cagcagggtc aatatcgata tgaacgatgc tggacctgct 5161 agcaaaagtc tccagtttac cagtcactcg atcgtcgaaa cgcactccaa atgccaacaa 5221 cagatctgct tggtccacgg cgtagttggc gtaaacagtt ccatgcattc caagcatttt 5281 caaacttaat ggatcagact cgggatacgc tcccaaaccc attaaagtcg atgtgatagg 5341 aattccggta agctcgacaa attccctcaa ctcttcgctg gcgtgattgc aacctcctcc 5401 agaatacact acaggcttct tagaactaga aatgagtctt ataatctgct cgattaacgt 5461 tggttccggt ggccgaggca gtcgttgtaa gtagcccttc agttgcattg gctgagacca 5521 atctggaata gccatttgct gttgcacgtc cttgggaata tctaccaaga ctgggcccgg 5581 cctacctgaa gctgccaaaa agaaagcctc cctgattacg cgagggatgt catcaatgtt 5641 cataacaagg taattgtgct tggtgatcga tctcgtaacc tccacaattg gagtttcttg 5701 aaaagcatcg gtaccaatca tcctactgga tacttgacct gtaatggcca ctagtggcac 5761 actgtccaag agggcatcag cgagcccggt aacaagattg gtcgctccag gaccggatgt 5821 ggctatacaa acccctactc gtccagacga tttagcataa ccttcagcag cgaaaatctc 5881 cccctgctcg tggcgacaga gaatgttgcg gatgctgctg gaacgtgtaa gagcctggtg 5941 aatctccata gacgctccgc cgggataagc gaacaccgtt ctaatccctt ctcgctcaag 6001 agcctccacc aaaatgtcgg ctcctttgcg gggctcgtca tccttgtagc gagactgaaa 6061 gtcggacggg gttggtgcaa cgagtgactt cccgctgcta agccgtggtc cactcacatc 6121 gactgcagac tggatcttct cgctaacagc ttgggctttc acttccagtg aacgctcagt 6181 cttccgagag gtgttgtatg catgtcccct cagagcggct cccgagaaga aaccactcct 6241 cacctttagc tctttagctc ccttgcaaca agaccttgcc gatggctgca ccttctccag 6301 gctcgctatg ctcaaaggca cagctgcgca tccactaatt gacgccatgc tcgagagaga 6361 tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc 6421 ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt 6481 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac 6541 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac 6601 gatagccttt cctttatcgc aattatggca tttgtaggtg ccaccttcct tttctactgt 6661 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac 6721 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt 6781 ggagtagacg agagtgtcgt gctccaccat gttcacatca atccacttgc tttgaagacg 6841 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac 6901 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt 6961 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga 7021 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt 7081 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg 7141 caagctgctc tagccaatac gcaaaccgaa ttcagaaata aattcagcct aattcggcgt 7201 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt 7261 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca 7321 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg 7381 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc 7441 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca 7501 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt 7561 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa 7621 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg 7681 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg 7741 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac 7801 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc 7861 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat 7921 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat 7981 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag 8041 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg 8101 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca 8161 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga 8221 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa 8281 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg 8341 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc 8401 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag 8461 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg 8521 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag 8581 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg 8641 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc 8701 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata 8761 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 8821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 8881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 8941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 9001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 9061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 9121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 9181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 9241 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 9301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 9361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 9421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 9481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 9541 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 9601 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 9661 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca 9721 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg 9781 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 9841 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg 9901 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga 9961 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa 10021 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct 10081 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt 10141 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt 10201 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac 10261 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc 10321 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg 10381 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc 10441 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac 10501 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct 10561 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg 10621 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg 10681 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt 10741 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc 10801 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct 10861 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca 10921 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg 10981 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg 11041 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg 11101 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta 11161 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg 11221 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt 11281 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc 11341 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt 11401 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag 11461 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc 11521 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc 11581 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc 11641 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc 11701 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct 11761 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc 11821 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa 11881 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa 11941 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa 12001 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt 12061 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct 12121 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg 12181 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg 12241 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg 12301 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg 12361 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc 12421 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga 12481 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc 12541 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc 12601 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc 12661 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc 12721 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg 12781 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc 12841 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc 12901 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc 12961 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg 13021 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc 13081 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac 13141 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc 13201 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg 13261 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc 13321 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg 13381 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca 13441 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg 13501 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg 13561 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc 13621 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga 13681 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct //