LOCUS Exported 5076 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBMTBX-2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5076) AUTHORS Prior JE, Lynch MD, Gill RT TITLE Broad-host-range vectors for protein expression across gram negative hosts. JOURNAL Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32. PUBMED 20148414 REFERENCE 2 (bases 1 to 5076) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5076 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 60..96 /label=soxR terminator /note="bidirectional E. coli soxR transcription terminator" rep_origin 1347..2116 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 2117..2779 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /label=pBBR1 Rep /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" CDS 2876..3691 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2949..2968) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 3708..3739 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" CDS complement(3767..4645) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 4672..4956 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 4879..4898 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" ORIGIN 1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt 121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct 181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct 241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa 301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc 361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt 421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt 481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt 541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct 601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct 661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct 721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg 781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga 841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt 901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc 961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt 1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca 1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt 1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg 1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc 1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc 1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt 1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag 1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc 1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc 1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg 1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc 1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt 1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc 1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg 1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa 1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc 1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt 2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta 2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa 2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc 2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa 2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt 2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg 2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga 2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga 2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca 2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc 2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc 2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt 2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc 2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag 2881 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga 2941 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 3001 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 3061 caatgatgtt acagatgaga tggtcaggct aaactggctg acggaattta tgcctcttcc 3121 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 3181 agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 3241 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 3301 cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttgg 3361 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 3421 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 3481 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 3541 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 3601 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 3661 gtttcacttg atgctcgatg agtttttctg agggcggatc cccctcaagt caaaagcctc 3721 cggtcggagg cttttgactt tctgctatgg aggtcaggta tgatttttat gacaacttga 3781 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg 3841 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga 3901 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt 3961 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg 4021 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt 4081 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact 4141 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct 4201 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat 4261 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt 4321 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc 4381 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac 4441 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga 4501 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt 4561 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca 4621 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca 4681 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa 4741 ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga 4801 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc 4861 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct 4921 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg gctagcaccg 4981 cctatctcgt gtgagatagg cggagatacg aactttaaga aggagatata cccatggtaa 5041 aatggtcagt attgagcgat atctagagaa ttcgtc //