LOCUS Exported 3845 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBTBXh-2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3845) AUTHORS Prior JE, Lynch MD, Gill RT TITLE Broad-host-range vectors for protein expression across gram negative hosts. JOURNAL Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32. PUBMED 20148414 REFERENCE 2 (bases 1 to 3845) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3845 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 4..21 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" terminator 74..110 /label=soxR terminator /note="bidirectional E. coli soxR transcription terminator" rep_origin 116..885 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 886..1548 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /label=pBBR1 Rep /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" CDS 1645..2460 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1718..1737) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 2477..2508 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" CDS complement(2536..3414) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 3441..3725 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 3648..3667 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" ORIGIN 1 gaccatcatc atcatcatca ttagagcttg atatcattca ggacgagcct cagactccag 61 cgtaactgga ctgaaaacaa actaaagcgc ccttgtggcg ctttagtttt gttccgcggc 121 caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc tgatggacag 181 gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca gccttcgacc 241 acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt tttttaattt 301 tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa 361 gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg 421 gaacgaccca agcctatgcg agtgggggca gtcgaaggcg aagcccgccc gcctgccccc 481 cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt gggcaaggcc 541 gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg agggggagcc 601 gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca aagaactaga 661 tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg gtacgcaaca 721 gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat ctgtaattga 781 ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc agctgattgc 841 gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga taagcatggc cacgcagtcc 901 agagaaatcg gcattcaagc caagaacaag cccggtcact gggtgcaaac ggaacgcaaa 961 gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca cggcggcaat gctgctgcat 1021 cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg tcagccagaa gacactttcc 1081 aagctcatcg gacgttcttt gcggacggtc caatacgcag tcaaggactt ggtggccgag 1141 cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg tgtcggccta cgtggtcaat 1201 gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc 1261 gtggtggttg atcacgacga ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc 1321 atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg gccccggcga ggagccgccc 1381 agccagcccg gcattccggg catggaacca gacctgccag ccttgaccga aacggaggaa 1441 tgggaacggc gcgggcagca gcgcctgccg atgcccgatg agccgtgttt tctggacgat 1501 ggcgagccgt tggagccgcc gacacgggtc acgctgccgc gccggtagta cgtaagaggt 1561 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga 1621 ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc ttgctcgagg 1681 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 1741 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg 1801 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcaggcta 1861 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat 1921 gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt attagaagaa 1981 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 2041 tcgattcctg tttgtaattg tccttttaac ggcgatcgcg tatttcgtct cgctcaggcg 2101 caatcacgaa tgaataacgg tttggttggt gcgagtgatt ttgatgacga gcgtaatggc 2161 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca 2221 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 2281 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 2341 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 2401 attgataatc ctgatatgaa taaattgcag tttcacttga tgctcgatga gtttttctga 2461 gggcggatcc ccctcaagtc aaaagcctcc ggtcggaggc ttttgacttt ctgctatgga 2521 ggtcaggtat gatttttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 2581 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 2641 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 2701 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 2761 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 2821 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 2881 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 2941 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 3001 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 3061 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 3121 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 3181 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 3241 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 3301 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 3361 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 3421 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 3481 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat 3541 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 3601 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 3661 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 3721 tccatacccg tttttttggg ctagcaccgc ctatctcgtg tgagataggc ggagatacga 3781 actttaagaa ggagatatac ccatggtaaa atggtcagta ttgagcgata tctagagaat 3841 tcgtc //