LOCUS Exported 3691 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBTBXh-3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3691) AUTHORS Prior JE, Lynch MD, Gill RT TITLE Broad-host-range vectors for protein expression across gram negative hosts. JOURNAL Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32. PUBMED 20148414 REFERENCE 2 (bases 1 to 3691) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3691 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 4..21 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" terminator 74..110 /label=soxR terminator /note="bidirectional E. coli soxR transcription terminator" rep_origin 116..885 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 886..1548 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /label=pBBR1 Rep /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" CDS 1645..2304 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1711..1730) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 2323..2354 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" CDS complement(2382..3260) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 3287..3571 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 3494..3513 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" ORIGIN 1 gaccatcatc atcatcatca ttagagcttg atatcattca ggacgagcct cagactccag 61 cgtaactgga ctgaaaacaa actaaagcgc ccttgtggcg ctttagtttt gttccgcggc 121 caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc tgatggacag 181 gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca gccttcgacc 241 acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt tttttaattt 301 tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa 361 gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg 421 gaacgaccca agcctatgcg agtgggggca gtcgaaggcg aagcccgccc gcctgccccc 481 cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt gggcaaggcc 541 gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg agggggagcc 601 gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca aagaactaga 661 tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg gtacgcaaca 721 gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat ctgtaattga 781 ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc agctgattgc 841 gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga taagcatggc cacgcagtcc 901 agagaaatcg gcattcaagc caagaacaag cccggtcact gggtgcaaac ggaacgcaaa 961 gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca cggcggcaat gctgctgcat 1021 cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg tcagccagaa gacactttcc 1081 aagctcatcg gacgttcttt gcggacggtc caatacgcag tcaaggactt ggtggccgag 1141 cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg tgtcggccta cgtggtcaat 1201 gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc 1261 gtggtggttg atcacgacga ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc 1321 atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg gccccggcga ggagccgccc 1381 agccagcccg gcattccggg catggaacca gacctgccag ccttgaccga aacggaggaa 1441 tgggaacggc gcgggcagca gcgcctgccg atgcccgatg agccgtgttt tctggacgat 1501 ggcgagccgt tggagccgcc gacacgggtc acgctgccgc gccggtagta cgtaagaggt 1561 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga 1621 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat 1681 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc 1741 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag 1801 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa 1861 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac 1921 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat 1981 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc 2041 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt 2101 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc 2161 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat 2221 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc 2281 gatgagtggc agggcggggc gtaaacgcgt ggatccccct caagtcaaaa gcctccggtc 2341 ggaggctttt gactttctgc tatggaggtc aggtatgatt tttatgacaa cttgacggct 2401 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat 2461 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg 2521 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg 2581 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc 2641 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg 2701 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta 2761 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa 2821 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc 2881 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc 2941 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc 3001 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt 3061 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg 3121 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc 3181 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga 3241 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt 3301 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa 3361 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa 3421 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc 3481 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc 3541 tttttatcgc aactctctac tgtttctcca tacccgtttt tttgggctag caccgcctat 3601 ctcgtgtgag ataggcggag atacgaactt taagaaggag atatacacat gttaaaatgg 3661 tcagtattga gcgatatcta gagaattcgt c //