LOCUS       Exported                7983 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses MLL3-PRKAG2 fusion gene.
ACCESSION   .
VERSION     .
KEYWORDS    pMXs-Puro- MLL3-PRKAG2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Yao F, Kausalya JP, Sia YY, Teo AS, Lee WH, Ong AG, Zhang Z, Tan JH,
            Li G, Bertrand D, Liu X, Poh HM, Guan P, Zhu F, Pathiraja TN, 
            Ariyaratne PN, Rao J, Woo XY, Cai S, Mulawadi FH, Poh WT, Veeravalli
            L, Chan CS, Lim SS, Leong ST, Neo SC, Choi PS, Chew EG, Nagarajan N,
            Jacques PE, So JB, Ruan X, Yeoh KG, Tan P, Sung WK, Hunziker W, Ruan
            Y, Hillmer AM
  TITLE     Recurrent Fusion Genes in Gastric Cancer: CLDN18-ARHGAP26 Induces 
            Loss of Epithelial Integrity.
  JOURNAL   Cell Rep. 2015 Jul 14;12(2):272-85. doi: 
            10.1016/j.celrep.2015.06.020. Epub 2015 Jul 2.
  PUBMED    26146084
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2942..2953
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4260..4286
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4323..4343)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4328..4657
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4508..4643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4570..4589
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4667..5266
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4667..4686)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5163..5183
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5488..5957
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6087..6104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6258..6846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6338..6357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7010..7870)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7633..7652
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7871..7975)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccgccgccac catgtcgtcg gaggaggaca agagcgtgga gcagccgcag ccgccgccac
     1981 caccccccga ggagcctgga gccccggccc cgagccccgc agccgcagac aaaagacctc
     2041 ggggccggcc tcgcaaagat ggcgcttccc ctttccagag agccagaaag aaacctcgaa
     2101 gtagggggaa aactgcagtg gaagatgagg acagcatgga tgggctggag acaacagaaa
     2161 cagaaacgat tgtggaaaca gaaatcaaag aacaatctgc agaagaggat gctgaagcag
     2221 aagtggataa cagcaaacag ctaattccaa ctcttcagcg atctgtgtct gaggaatcgg
     2281 caaactccct ggtctctgtt ggtgtagaag ccaaaatcag tgaacagctc tgcgcttttt
     2341 gttactgtgg ggaaaaaagt tccttaggac aaggagactt aaaacaattc agaataacgc
     2401 ctggatttat cttgccatgg agaaaccaac cttctaacaa gaaggacatt gatgacaaca
     2461 gcaatggaac ctatgagaaa atgcaaaact cagcaccacg aaaacaaaga ggacagagaa
     2521 aagaacgatc tcctcagcag aatatagtat cttgtgtaag tgtaagcacc cagacagctt
     2581 cagatgatca agctggtaaa ctgtgggatg aactcagtct ggttgggctt ccagatgcca
     2641 ttgatatcca agccttattt gattctacag gcacttgttg ggctcatcac cgttgtgtgg
     2701 agtggtcact aggagtatgc cagatggaag aaccattgtt agtgaacgtg gacaaagctg
     2761 ttgtctcagg gagcacagaa cgatgtgcat tttgtaagca ccttggagcc actatcaaat
     2821 gctgtgaaga gaaatgtacc cagatgtatc attatccttg tgctgcagga gccggcacct
     2881 ttcaggattt cagtcacatc ttcctgcttt gtccagaaca cattgaccaa gctcctgaaa
     2941 gatcgaagga agatgcaaac tgtgcagtgt gcgacagccc gggagacctc ttagatcagt
     3001 tcttttgtac tacttgtggt cagcactatc atggaatgtg cctggatata gcggttactc
     3061 cattaaaacg tgcaggttgg caatgtcctg agtgcaaagt gtgccagaac tgcaaacaat
     3121 cgggagaaga tagcaagatg ctagtgtgtg atacgtgtga caaagggtat catacttttt
     3181 gtcttcaacc agttatgaaa tcagtaccaa ccaatggctg gaaatgcaaa gcggcgctgg
     3241 cggcggccct gggacccgcg gaagccggca tgctggagaa gctggagttc gaggacgaag
     3301 cagtagaaga ctcagaaagt ggtgtttaca tgcgattcat gaggtcacac aagtgttatg
     3361 acatcgttcc aaccagttca aagcttgttg tctttgatac tacattacaa gttaaaaagg
     3421 ccttctttgc tttggtagcc aacggtgtcc gagcagcgcc actgtgggag agtaaaaaac
     3481 aaagttttgt aggaatgcta acaattacag atttcataaa tatactacat agatactata
     3541 aatcacctat ggtacagatt tatgaattag aggaacataa aattgaaaca tggagggagc
     3601 tttatttaca agaaacattt aagcctttag tgaatatatc tccagatgca agcctcttcg
     3661 atgctgtata ctccttgatc aaaaataaaa tccacagatt gcccgttatt gaccctatca
     3721 gtgggaatgc actttatata cttacccaca aaagaatcct caagttcctc cagcttttta
     3781 tgtctgatat gccaaagcct gccttcatga agcagaacct ggatgagctt ggaataggaa
     3841 cgtaccacaa cattgccttc atacatccag acactcccat catcaaagcc ttgaacatat
     3901 ttgtggaaag acgaatatca gctctgcctg ttgtggatga gtcaggaaaa gttgtagata
     3961 tttattccaa atttgatgta attaatcttg ctgctgagaa aacatacaat aacctagata
     4021 tcacggtgac ccaggccctt cagcaccgtt cacagtattt tgaaggtgtt gtgaagtgca
     4081 ataagctgga aatactggag accatcgtgg acagaatagt aagagctgag gtccatcggc
     4141 tggtggtggt aaatgaagca gatagtattg tgggtattat ttccctgtcg gacattctgc
     4201 aagccctgat cctcacacca gcaggtgcca aacaaaagga gacagaaacg gagctcgagt
     4261 acccatacga tgttccagat tacgcttgat gagcggccgc cagcacagtg gtcgacagct
     4321 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4381 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4501 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4561 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4621 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa
     4681 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     4741 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     4801 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     4861 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     4921 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     4981 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5041 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5101 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5161 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgagtgccc
     5221 gaaggaccgc gcgacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     5281 cccgcagcgc ccgaccgaaa ggagcgcacg acccatcgtc gacgataaaa taaaagattt
     5341 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     5401 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca
     5461 gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     5521 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
     5581 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     5641 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     5701 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     5761 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca
     5821 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
     5881 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
     5941 tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt
     6001 atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6061 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6121 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgcgg
     6961 ccggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7081 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7141 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7981 ttc
//
