LOCUS Exported 4457 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2). ACCESSION . VERSION . KEYWORDS CMV-G-GECO1.2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4457) AUTHORS Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE TITLE An Expanded Palette of Genetically Encoded Ca2+ Indicators. JOURNAL Science. 2011 Sep 8. PUBMED 21903779 REFERENCE 2 (bases 1 to 4457) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4457 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 42..266 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 255..272 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" promoter 292..310 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 292..309 /label=SP6 /note="SP6 promoter, forward primer" CDS complement(371..814) /codon_start=1 /product="calcium-binding protein" /label=calmodulin /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK" primer_bind complement(1322..1343) /label=EGFP-C /note="EGFP, forward primer" CDS complement(1556..1612) /codon_start=1 /product="calmodulin-binding peptide from avian smooth muscle myosin light chain kinase" /label=calmodulin-binding peptide /translation="RRKWNKTGHAVRAIGRLSS" primer_bind complement(1661..1680) /label=T7 /note="T7 promoter, forward primer" promoter complement(1662..1680) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(1725..1928) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(1754..1774) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 1929..2308 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind 2480..2499 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 2574..2678 /gene="bla" /label=AmpR promoter CDS 2679..3539 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2897..2916) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3710..4298 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4199..4218 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttccggacta gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc 61 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc 121 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga 181 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac 241 agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag catttaggtg 301 acactataga atagggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc 361 tgcagaattc ttacttcgct gtcatcattt gtacaaactc ttcgtagttt acctgaccat 421 ccccatcgat gtctgctacc ctgatcattt catcaacctc ttcatctgtt aacttctctc 481 caaggtttgt catcacgtgg cgaagctctg ctgcgccgat gtagccattg ccgtccttat 541 caaacacacg gaacgcttct ctaatttctt cttcactgtc tgtgtctttc atttttcttg 601 ccatcattgt caggaactca gggaagtcga ttgtgccgtc accgtcggca tctacttcat 661 tgatcatgtc ctgcagctct gcttctgtgg ggttctgccc cagagaccgc atcaccgtcc 721 ccagctcctt ggttgttatc gtcccatccc cgtccttgtc aaatagggag aaagcctctt 781 taaattctgc gatctgctct tcagtcagtt ggtcacgcgt gttgtactcc agcttgtgcc 841 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg 901 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgccc ttgaagaaga 961 tggtgcgctc ctggatgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca 1021 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg 1081 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg 1141 tggcatcgcc ctcaccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca 1201 gctcgacctg gatgggcacc accccggtga acagctcctc gcccttgctc accatggatt 1261 cgctccctcc ggtaccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg 1321 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttc gaaagcatgg 1381 actgcacgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt 1441 tctgctggta gtggtaggcg agctgcacgc cgccgccctc gatgttgtgg cggatcttga 1501 agtacgcctt gatgccgttc ttctgctcgt cggccttgat atagacgttc tcgagtgagc 1561 tcagccgacc tatagctctg actgcgtgac ctgtcttatt ccacttacga cgtgatgagt 1621 cgaccatggt ggatccgagc tcggtaccaa gcttgggtct ccctatagtg agtcgtatta 1681 atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag 1741 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat 1801 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg 1861 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac 1921 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc 1981 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata 2041 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa 2101 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc 2161 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta 2221 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac 2281 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc 2341 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt 2401 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc 2461 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga 2521 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa 2581 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 2641 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 2701 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 2761 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 2821 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 2881 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 2941 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 3001 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 3061 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 3121 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 3181 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 3241 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 3301 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 3361 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 3421 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 3481 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 3541 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 3601 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 3661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 3721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 3781 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 3841 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 3901 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 3961 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 4021 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 4081 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 4141 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 4201 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 4261 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 4321 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 4381 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 4441 cgaccgagcg cagcgag //