LOCUS Exported 2685 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMLLCK-K112503 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2685) TITLE J.C. Anderson Lab Plasmids REFERENCE 2 (bases 1 to 2685) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2685 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 6..35 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" primer_bind complement(65..82) /label=L4440 /note="L4440 vector, forward primer" protein_bind complement(144..168) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(318..337) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 329..1144 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(402..421) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" CDS 1354..2013 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1420..1439) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 2034..2128 /label=lambda t0 terminator /note="transcription terminator from phage lambda" rep_origin 2164..2520 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" protein_bind 2582..2606 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gatctgaaca aaaactcatc tcagaagagg atctgtaagg atcctaactc gctcctcagg 61 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 121 actcaaaggc ggtaatcaat tcgacccagc tttcttgtac aaagtggttg atcggatctg 181 ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa 241 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 301 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc 361 caggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 421 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga 481 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 541 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 601 tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga 661 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 721 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 781 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 841 tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga 901 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 961 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 1021 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 1081 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 1141 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga 1201 ggatctctga agactctcga gctgcaggac tcacagctcc tcggatcttt gatcgggcac 1261 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag 1321 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc 1381 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct 1441 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag 1501 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct 1561 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac 1621 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac 1681 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa 1741 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc 1801 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc 1861 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt 1921 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa 1981 cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct 2041 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg 2101 ccgccgggcg ttttttattg gtgagaatcc aagcctcgga tctagcagtt caacctgttg 2161 atagtacgta ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc 2221 tctcatgttt aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta 2281 ctaagctctc atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat 2341 cagcaactta aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg 2401 cttttaaagc ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga 2461 gaagccctta gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca 2521 tgggaattag ccatgggccc gtgcgaatca cggatctccc gatcaagaat tgccattatc 2581 aacaagtttg tacaaaaaag caggctccga attggtatca cgaggcagaa tttcagataa 2641 aaaaaatcct tagctttcgc taaggatctg aagtggaatt catga //