LOCUS Exported 2682 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMLLCK-J72033 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2682) TITLE J.C. Anderson Lab Plasmids REFERENCE 2 (bases 1 to 2682) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2682 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 9..32 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" primer_bind complement(62..79) /label=L4440 /note="L4440 vector, forward primer" protein_bind complement(141..165) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(315..334) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 326..1141 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(399..418) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" CDS 1351..2010 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1417..1436) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 2031..2125 /label=lambda t0 terminator /note="transcription terminator from phage lambda" rep_origin 2161..2517 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" protein_bind 2579..2603 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gatctacctg gagccacccg cagttcgaaa aataaggatc ctaactcgct cctcaggctt 61 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 121 caaaggcggt aatcaattcg acccagcttt cttgtacaaa gtggttgatc ggatctgctg 181 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct 241 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta 301 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctccag 361 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 421 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt 481 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 541 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 601 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga 661 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 721 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 781 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 841 ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc 901 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat 961 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct 1021 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg 1081 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta 1141 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggagga 1201 tctctgaaga ctctcgagct gcaggactca cagctcctcg gatctttgat cgggcacgta 1261 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta 1321 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc 1381 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 1441 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 1501 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 1561 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 1621 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac 1681 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac 1741 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg 1801 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 1861 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 1921 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag 1981 tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt 2041 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg 2101 ccgggcgttt tttattggtg agaatccaag cctcggatct agcagttcaa cctgttgata 2161 gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt actaagctct 2221 catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta 2281 agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag 2341 caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt 2401 ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa 2461 gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg gctgacatgg 2521 gaattagcca tgggcccgtg cgaatcacgg atctcccgat caagaattgc cattatcaac 2581 aagtttgtac aaaaaagcag gctccgaatt ggtatcacga ggcagaattt cagataaaaa 2641 aaatccttag ctttcgctaa ggatctgaag tggaattcat ga //