LOCUS Exported 2697 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMLLCK-J72035 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2697) TITLE J.C. Anderson Lab Plasmids REFERENCE 2 (bases 1 to 2697) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2697 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 6..47 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind complement(77..94) /label=L4440 /note="L4440 vector, forward primer" protein_bind complement(156..180) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(330..349) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 341..1156 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(414..433) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" CDS 1366..2025 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1432..1451) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 2046..2140 /label=lambda t0 terminator /note="transcription terminator from phage lambda" rep_origin 2176..2532 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" protein_bind 2594..2618 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gatctggtaa gcctatccct aaccctctgc tgggtctcga ttctacgtaa ggatcctaac 61 tcgctcctca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 121 cggtatcagc tcactcaaag gcggtaatca attcgaccca gctttcttgt acaaagtggt 181 tgatcggatc tgctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt 241 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat 301 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga 361 aacgtcttgc tccaggccgc gattaaattc caacatggat gctgatttat atgggtataa 421 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc 481 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 541 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 601 tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt 661 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 721 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 781 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 841 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc 901 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 961 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 1021 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 1081 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 1141 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct 1201 gacttgacgg gaggatctct gaagactctc gagctgcagg actcacagct cctcggatct 1261 ttgatcgggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc 1321 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc 1381 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt 1441 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta 1501 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc 1561 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg 1621 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc 1681 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg 1741 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc 1801 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac 1861 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg 1921 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt 1981 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatttga tatcgagctc 2041 gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag 2101 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagcctcg gatctagcag 2161 ttcaacctgt tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt 2221 aacgtactaa gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc 2281 atggctaacg tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa 2341 attaatataa atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa 2401 gaatatataa ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat 2461 gtaacgcact gagaagccct tagagcctct caaagcaatt ttgagtgaca caggaacact 2521 taacggctga catgggaatt agccatgggc ccgtgcgaat cacggatctc ccgatcaaga 2581 attgccatta tcaacaagtt tgtacaaaaa agcaggctcc gaattggtat cacgaggcag 2641 aatttcagat aaaaaaaatc cttagctttc gctaaggatc tgaagtggaa ttcatga //