LOCUS Exported 10577 bp ds-DNA circular SYN 29-JUL-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMJ841 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10577) AUTHORS Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E TITLE A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. JOURNAL Science. 2012 Jun 28. PUBMED 22745249 REFERENCE 2 (bases 1 to 10577) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10577 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 30..49 /label=T7 /note="T7 promoter, forward primer" promoter 30..48 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 49..73 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 88..110 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 121..138 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 142..1239 /codon_start=1 /gene="malE (mutated)" /product="maltose binding protein from E. coli" /label=MBP /note="This version of the gene does not encode a signal sequence, so MBP will remain in the cytosol." /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW YAVRTAVINAASGRQTVDEALKDAQT" primer_bind 1213..1236 /label=MBP-F /note="Maltose binding protein, forward primer" CDS 1270..1290 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 1300..5406 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5471..5488 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(5541..5559) /label=T7 Term /note="T7 terminator, reverse primer" terminator 5555..5602 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 5639..6094 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5726..5745) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5936..5957 /label=F1ori-F /note="F1 origin, forward primer" CDS complement(6186..7001) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 6909..6928 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 6993..7012 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin 7123..7711 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7612..7631 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7865..7882 /label=L4440 /note="L4440 vector, forward primer" misc_feature 7897..8036 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(7962..7981) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8122..8144 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(8138..8329) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" CDS complement(9138..10220) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 10182..10201 /label=LacI-R /note="LacI, reverse primer" promoter complement(10221..10298) /gene="lacI" /label=lacI promoter primer_bind 10504..10523 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" ORIGIN 1 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc 61 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg 121 caccatcacc atcaccatgg aaaaatcgaa gaaggtaaac tggtaatctg gattaacggc 181 gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt 241 aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tcccacaggt tgcggcaact 301 ggcgatggcc ctgacattat cttctgggca cacgaccgct ttggtggcta cgctcaatct 361 ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc 421 tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta 481 tcgctgattt ataacaaaga tctgctgccg aacccgccaa aaacctggga agagatcccg 541 gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa 601 ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac 661 ggcaagtacg acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc 721 ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca 781 gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc 841 aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa 901 ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccgaacaaa 961 gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt 1021 aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa 1081 gatccacgta ttgccgccac tatggaaaac gcccagaaag gtgaaatcat gccgaacatc 1141 ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt 1201 cgtcagactg tcgatgaagc cctgaaagac gcgcagacta attcgagctc gaacaacaac 1261 aacactagtg aaaacctgta tttccaggga gcagcctcga tggataagaa atactcaata 1321 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt 1381 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt 1441 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca 1501 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca 1561 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg 1621 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct 1681 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat 1741 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat 1801 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag 1861 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat 1921 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct 1981 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt 2041 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca 2101 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct 2161 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga 2221 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa 2281 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat 2341 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct 2401 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag 2461 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac 2521 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa 2581 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga 2641 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg 2701 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca 2761 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta 2821 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc 2881 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc 2941 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat 3001 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt 3061 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg 3121 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa 3181 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg 3241 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt 3301 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt 3361 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac 3421 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta 3481 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg 3541 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat 3601 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt 3661 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa 3721 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa 3781 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt 3841 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg 3901 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta 3961 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt 4021 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc 4081 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat 4141 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga 4201 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg 4261 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag 4321 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa 4381 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa 4441 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg 4501 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg 4561 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag 4621 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat 4681 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct 4741 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca 4801 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat 4861 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa 4921 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct 4981 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt 5041 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat 5101 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta 5161 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa 5221 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt 5281 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt 5341 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt 5401 gactaacgag gccggtgctt tgcaggatcc gaattcgagc tccgtcgaca agcttgcggc 5461 cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga 5521 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa 5581 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac 5641 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 5701 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 5761 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 5821 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 5881 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 5941 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 6001 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 6061 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc 6121 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt 6181 aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta 6241 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag 6301 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata 6361 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg 6421 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca 6481 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt 6541 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga 6601 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca 6661 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat 6721 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc 6781 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc 6841 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc 6901 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat 6961 cgcggcctag agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg 7021 tttatgtaag cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt 7081 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 7141 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 7201 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 7261 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 7321 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 7381 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 7441 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 7501 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 7561 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 7621 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 7681 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 7741 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 7801 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 7861 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac 7921 gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc 7981 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 8041 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 8101 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 8161 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga 8221 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc 8281 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg 8341 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 8401 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac 8461 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 8521 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 8581 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 8641 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 8701 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 8761 tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg 8821 cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt 8881 gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt 8941 cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga 9001 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg 9061 ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta 9121 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 9181 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt 9241 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag 9301 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg 9361 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata 9421 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga 9481 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt 9541 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg 9601 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg 9661 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc 9721 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga 9781 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc 9841 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta 9901 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg 9961 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg 10021 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg 10081 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg 10141 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg 10201 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat 10261 gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt 10321 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc 10381 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc 10441 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc 10501 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc 10561 cacgatgcgt ccggcgt //