LOCUS       Exported                 829 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  3rd generation lentiviral plasmid for inducible expression of shRNA;
            neomycin selection. See manual for detailed protocols. No further 
            technical support is available from the depositing laboratory..
ACCESSION   .
VERSION     .
KEYWORDS    Tet-pLKO-neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Wiederschain D, Wee S, Chen L, Loo A, Yang G, Huang A, Chen Y, 
            Caponigro G, Yao YM, Lengauer C, Sellers WR, Benson JD
  TITLE     Single-vector inducible lentiviral RNAi system for oncology target 
            validation.
  JOURNAL   Cell Cycle. 2009 Feb 1. 8(3):498-504.
  PUBMED    19177017
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(194..213)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     804..823
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
       61 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      121 aacacgatga taaggatcca tgattgaaca agatggattg cacgcaggtt ctccggccgc
      181 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
      241 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
      301 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
      361 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
      421 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
      481 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
      541 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
      601 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
      661 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
      721 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
      781 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaa
//
