LOCUS       Exported                 819 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  3rd generation lentiviral plasmid for inducible expression of shRNA;
            puromycin selection. See manual for detailed protocols. No further 
            technical support is available from the depositing laboratory..
ACCESSION   .
VERSION     .
KEYWORDS    Tet-pLKO-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Wiederschain D, Wee S, Chen L, Loo A, Yang G, Huang A, Chen Y, 
            Caponigro G, Yao YM, Lengauer C, Sellers WR, Benson JD
  TITLE     Single-vector inducible lentiviral RNAi system for oncology target 
            validation.
  JOURNAL   Cell Cycle. 2009 Feb 1. 8(3):498-504.
  PUBMED    19177017
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             155..754
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(155..174)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     651..671
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
       61 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
      121 aaaacacgat gataaggatc caccggagct taccatgacc gagtacaagc ccacggtgcg
      181 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
      241 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
      301 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
      361 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
      421 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
      481 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
      541 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
      601 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
      661 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
      721 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
      781 gaccgaaagg agcgcacgac cccatgcatc ggtaccttt
//
