LOCUS Exported 8882 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pJFRC12-10XUAS-IVS-myr::GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8882) AUTHORS Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, Rubin GM TITLE Refinement of Tools for Targeted Gene Expression in Drosophila. JOURNAL Genetics. 2010 Aug 9. ():. PUBMED 20697123 REFERENCE 2 (bases 1 to 8882) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8882 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 31..135 /gene="bla" /label=AmpR promoter CDS 136..996 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(354..373) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1167..1755 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1656..1675 /label=pBR322ori-F /note="pBR322 origin, forward primer" protein_bind 1842..1911 /label=attB /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" primer_bind complement(2136..2154) /label=pCasper-F /note="Drosophila mini-white gene, reverse primer" CDS join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793, 4864..5478) /codon_start=1 /gene="white" /product="Drosophila white gene eye color pigment" /label=mini-white /note="This is a modified version of the white gene lacking part of the first intron." /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE" protein_bind 6508..6602 /label=5X UAS /bound_moiety="GAL4" /note="five tandem copies of the ""ScaI site"" 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" protein_bind 6636..6730 /label=5X UAS /bound_moiety="GAL4" /note="five tandem copies of the ""ScaI site"" 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" promoter 6761..6999 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" primer_bind 6946..6967 /label=pCasper-hs /note="Drosophila Hsp70 promoter, forward primer" intron 7014..7080 /label=Mhc intron /note="modified Drosophila myosin heavy chain intron 16 (Pfeiffer et al., 2010)" CDS 7365..8078 /codon_start=1 /product="green fluorescent protein" /label=GFP /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" intron 8165..8230 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 8360..8380 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 8652..8786 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8689..8708) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8743..8762 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8828..8846) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct 6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg 6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt 6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact 6721 gtcctccgag cggagactct agcgctagcg acgtcgagcg ccggagtata aatagaggcg 6781 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa 6841 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt 6901 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc 6961 tgccaagaag taattattga atacaagaag agaactctga atagatctaa aaggtaggtt 7021 caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc tttacttcag 7081 gcggccgcgg ctcgagaatc aaaatgggca acaaatgctg cagcaagcga caggatcagg 7141 aactggcact ggcctatccc actgggggct acaagaaatc cgactacacc tttggccaga 7201 cgcacatcaa cagcagcggc ggcggcaaca tgggcggcgt tcttggccag aagcataaca 7261 acggtggctc gctggactcg cgctacacgc ccgatcccaa tcatcggggt ccgttgaaaa 7321 tcggcggaaa gggcggcgtt gacatcatca gaccacgcgg atccatgtcc aaaggtgaag 7381 aactgtttac cggagtagtc ccgatattgg ttgaactcga cggcgatgtc aacggtcata 7441 aattcagtgt gtccggcgag ggtgagggcg acgccacata cggtaagctg acgttgaagt 7501 tcatatgcac cacgggcaag ctgcccgtgc catggccgac gttggtcacg acgctgacgt 7561 atggtgtcca gtgtttcagc cgttaccccg atcatatgaa gcagcatgac tttttcaagt 7621 cggcgatgcc ggagggatac gttcaagaga ggaccatttt cttcaaggat gacggcaact 7681 ataagacgcg agcggaggtg aaatttgaag gcgacacact cgttaaccgt attgagttga 7741 agggcattga ttttaaggag gatggcaaca ttctgggcca taagttggaa tacaactaca 7801 actcgcataa tgtgtatata atggcagata agcagaaaaa cggaataaag gttaacttca 7861 agattcgcca caacatagag gacggttccg tgcaacttgc agatcattac caacagaaca 7921 cacccattgg agatggccca gttctcttgc cagacaatca ctacctttcc acacagtccg 7981 cgttgagcaa ggaccccaat gaaaagcggg accacatggt gttgctggag tttgtgaccg 8041 cagctggtat tacacacggc atggatgagc tctacaagta atctagagga tctttgtgaa 8101 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc 8161 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg 8221 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa 8281 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga 8341 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc 8401 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt 8461 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata 8521 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt 8581 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 8641 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 8701 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 8761 tcaatgtatc ttatcatgtc tggatcgatc tggccggccg tttaaacgaa ttcttgaaga 8821 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 8881 ta //