LOCUS       Exported                9361 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBPZpGAL4DBDUw
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of Tools for Targeted Gene Expression in Drosophila.
  JOURNAL   Genetics. 2010 Aug 9. ():.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        232..262
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2856..2872)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2856..2872)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(3883..4497,4568..4699,4903..5218,5280..5934,
                     6009..6282,6684..6755))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7207..7225
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    7450..7519
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(7606..8194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7686..7705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8365..9225)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8988..9007
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9226..9330)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accaaaacat gctggagatc
     1981 cgcgccgcct tcctgcgcca gcgcaacacc gccctgcgca ccgaggtggc cgagctggag
     2041 caggaggtgc agcgcctgga gaacgaggtg agccagtacg agacccgcta cggccccctg
     2101 ggcggcggca aggctagcgg aggaggtggt ggaggtggag gtggaggtac tagtatgaag
     2161 ctgctgagta gtattgaaca agcgtgtgac atatgccgac ttaagaagct gaagtgcagc
     2221 aaggaaaagc cgaagtgcgc caaatgtctt aagaacaatt gggagtgccg gtactcgcca
     2281 aagacgaagc gaagcccttt gacacgcgct catttgactg aagtggagag tcgcctggag
     2341 cgccttgagc agttgttcct cctcattttt ccccgtgaag acctcgacat gatactgaag
     2401 atggatagtc tccaagatat taaggccctg ctcactggcc tgtttgttca ggataacgtc
     2461 aacaaggatg ccgtgacgga ccgccttgcc tcggtggaga cagatatgcc acttaccttg
     2521 cggcagcatc gcatctcggc taccagcagt agtgaagaaa gttccaataa gggtcaacgg
     2581 caactgacgg tatcgtaaaa gcttatcgat accgtcgact aaagccaaat agaaaattat
     2641 tcagttcctg gcttaagttt ttaaaagtga tattatttat ttggttgtaa ccaaccaaaa
     2701 gaatgtaaat aactaataca taattatgtt agttttaagt tagcaacaaa ttgattttag
     2761 ctatattagc tacttggtta ataaatagaa tatatttatt taaagataat tgcgttttta
     2821 ttgtcaggga gtgagtttgc ttaaaaactc gtttagatcc actagttcta gagcggccgc
     2881 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
     2941 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
     3001 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
     3061 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggaggcg cgccctagtt
     3121 ccagtgaaat ccaagcattt tctaaattaa atgtattctt attattatag ttgttatttt
     3181 tgatatatat aaacaacact attatgccca ccattttttt gagatgcatc tacacaagga
     3241 acaaacactg gatgtcactt tcagttcaaa ttgtaacgct aatcactccg aacaggtcac
     3301 aaaaaattac cttaaaaagt cataatatta aattagaata aatatagctg tgagggaaat
     3361 atatacaaat atattggagc aaataaattg tacatacaaa tatttattac taatttctat
     3421 tgagacgaaa tgaaccactc ggaaccattt gagcgaaccg aatcgcgcgg aactaacgac
     3481 agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc ggagagcggg tgggagacag
     3541 cgaaagagca actacgaaac gtggtgtggt ggaggtgaat tatgaagagg gcgcgcgatt
     3601 tgaaaagtat gtatataaaa aatatatccc ggtgttttat gtagcgataa acgagttttt
     3661 gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag acaaatccaa agtctacttg
     3721 tggggatgtt cgaaggggaa atacttgtat tctataggtc atatcttgtt tttattggca
     3781 caaatataat tacattagct ttttgagggg gcaataaaca gtaaacacga tggtaataat
     3841 ggtaaaaaaa aaaaacaagc agttatttcg gatatatgtc ggctactcct tgcgtcgggc
     3901 ccgaagtctt agagccagat atgcgagcac ccggaagctc acgatgagaa tggccagacc
     3961 cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga tgaccttgcc
     4021 cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct ccacgtccgc
     4081 ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt acgacaacca
     4141 tttgaggtat actggcaccg agcccgagtt caagaagaag ccgccaaaga gcaggaatgg
     4201 tatgataacc ggcggaccca cagacagcgc catcgaggtc gaggagctgg cgcaggatat
     4261 tagatatccg aaggacgttg acacattggc caccagagtg accagcgcca ggcagttgaa
     4321 gaagtgcagc actccggccc gcagtccgat catcggatag gcaatcgccg tgaagaccag
     4381 tggcactgtg agaaaaagcg gcaattcggc aatcgttttg cccagaaagt atgtgtcaca
     4441 gcgataaagt cgacttcggg cctccctcat aaaaactggc agctctgagg tgaacaccta
     4501 aatcgaatcg attcattaga aagttagtaa attattgaaa tgcaaatgta ttctaaacat
     4561 gacttacatt tatcgtggca aagacgtttt gaaaggtcat gttggtcagg aagaggaaga
     4621 tggctccgtt gatattcatc acacccactt gcgtgagttg ttggcccaaa aagatgaggc
     4681 caatcaagat ggcaaccatc tgcaaattaa aatgttactc gcatctcatt aatattcgcg
     4741 agttaaatga aatttattta tcttctgcaa aactataaac tatacatctc attgaaaaaa
     4801 actaagaagg gtgtggaatc aggcaattct atctaaaatc tagcgaattt gtttccaaga
     4861 attgtaagcg ttatatcatt tgtttccact ggaaccactc accgttgtct gaataagtcg
     4921 cacttttacg aggagtggtt ccttgagcac cgacagccag gatcgccaca ggaccgcccg
     4981 gaactgcatg aaccaggtgg ccttgtaggt gtacccattc tccggctgct ccagtggctt
     5041 ctccagattt ttggtggcca acaactgctc catatcccgg gctactttgc taatggcaaa
     5101 attgtcgcat atcttggcga tccgatcacg ggactcgatc tcccgtccgg gcacaacggc
     5161 caacacctgt acgtaaaagt ccgccggatt gtagttggta ggacactggg cacccacgct
     5221 ggataggagt tgagatgtta tgtaatacta gataccctta ataaacacat cgaactcact
     5281 aggaaaagaa gtcgacggct tcgctgggag tgcccaagaa agctaccctg ccctcggcca
     5341 tcagaaggat cttgtcaaag agctcaaaca gctcggaaga cggctgatga atggtcagga
     5401 tgacggtctt gcccttctgc gacagcttct tcagcacctg gacgacgctg tgggcggtaa
     5461 aggagtccag tccggaggtg ggctcatcgc agatcagaag cggcggatcg gttagagcct
     5521 cggaggcgaa tgccagacgc ttcctttctc cgccggacag acctttcacc ctgccgggca
     5581 caccgatgat cgtgtgctga catttgctga gcgaaagctc ctggatcacc tgatccacgc
     5641 gggccactcg ctgccgatag gtcagatgtc gtggcatccg caccatggct tggaaaatca
     5701 ggtgttccct ggccgttagg gagccgataa agaggtcatc ctgctggaca taggcgcacc
     5761 tggcctgcat ctccttggcg tccacaggtt ggccattgag cagtcgcatc ccggatggcg
     5821 atacttggat gccctgcggc gatcgaaagg caagggcatt cagcagggtc gtctttccgg
     5881 caccggaact gcccatcacg gccaaaagtt cgcccggata ggccacgccg caaactgagt
     5941 ttcaaattgg taattggacc ctttattaag atttcacaca gatcagccga ctgcgaatag
     6001 aaactcaccg ttcttgagca aatgtttcct gggcgccggt atgtgtcgct cgttgcagaa
     6061 tagtccgcgt gtccggttga ccagctgccg ccatccggag cccggctgat tgaccgcccc
     6121 aaagatgtcc atattgtgcc aggcataggt gaggttctcg gctagttggc cgctccctga
     6181 accggagtcc tccggcggac tgggtggccg gagcgtgccg tagtttttgg cctgcccgaa
     6241 gccctggtta atgcagctct gcgaagccgc tccgctgtca ccctgcaatg ataggggatc
     6301 tcaaatatca actactagcg ttatgctcat ctaaccccga acaaaaagta ccccgaagta
     6361 tcctacgaag taggtttata cttttattta ttttttgtgc atctaggatc agcttaaaat
     6421 atctggttgt tatatttttt gtaaaaaaga atatagtcga aaatgaatgc ctttagatgt
     6481 cttgatcatg atatgatctc aaaaattgtc ttatatagcg agaacagcta ccagaataat
     6541 ctgtttcgtg tcactatttg tttgtgcaat tgcggtttgg gatttttgtg ggtcgcagtt
     6601 ctcacgccgc atacaatttg atgttgcaat cgcagttcct atagatcaag tgaacttaag
     6661 atgtatgcac atgtactact cacattgttc agatgctcgg cagatgggtg tttgctgcct
     6721 ccgcgaatta atagctcctg atcctcttgg cccattgccg ggatttttca cactttcccc
     6781 tgcttaccca cccaaaacca atcaccaccc caatcactca aaaaacaaac aaaaataaga
     6841 agcgagagga gttttggcac agcactttgt gtttaattga tggcgtaaac cgcttggagc
     6901 ttcgtcacga aaccgctgac aaaatgcaac tgaaggcgga cattgacgct acgtaacgct
     6961 acaaacggtg gcgaaagaga tagcggacgc agcggcgaaa gagacggcga tatttctgtg
     7021 gacagagaag gaggcaaaca gcgctgactt tgagtggaat gtcattttga gtgagaggta
     7081 atcgaaagaa cctggtacat caaataccct tggatcgaag taaatttaaa actgatcaga
     7141 taagttcaat gatatccagt gcagtaaaaa aaaaaaatgt tttttttatc tactttccgc
     7201 aaaaatgggt tttattaact tacatacata ctaggcgcgc ccatatgttc ggcttgtcga
     7261 catgcccgcc gtgaccgtcg agaacccgct gacgctgccc cgcgtatccg cacccgccga
     7321 cgccgtcgca cgtcccgtgc tcaccgtgac caccgcgccc agcggtttcg agggcgaggg
     7381 cttcccggtg cgccgcgcgt tcgccgggat caactaccgc cacctcgacc cgttcatcat
     7441 gatggaccag atgggtgagg tggagtacgc gcccggggag cccaagggca cgccctggca
     7501 cccgcaccgc ggcttcgaga ccgtgaccta catcgtcgac ggtaacatgt gagcaaaagg
     7561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8041 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8281 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8341 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8461 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8521 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8581 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8821 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8881 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9001 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9061 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9121 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9361 c
//
