LOCUS       Exported                 819 bp ds-DNA     linear   SYN 01-SEP-2021
DEFINITION  Phosphoserine orthogonal translation system.
ACCESSION   .
VERSION     .
KEYWORDS    SepOTS-eta
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        703..780
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(800..819)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tatggtattg ataatcctga tatgagtaaa ttgcagtttc atttgatgct cgatgagttt
       61 ttctaagaat taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      121 aggggttccg cgcacatttc cccgaaaagt gccaccgcgg ccgcaaaaaa aatccttagc
      181 tttcgctaag gatctgcagg tcgactctag aggatcctgg agccgggggt gggatttgaa
      241 cccacgtaag gcggatctag agtccgctgc ctaaccccta gactaccccg gcgaattcag
      301 cgttacaagt attacacaaa gttttttatg ttgagaatat ttttttgatg gggcgccact
      361 tatttttgat cgttcgctca aagaagcgcg gccgcaaaaa aaatccttag ctttcgctaa
      421 ggatctgcag gtcgactcta gaggatcctg gagccggggg tgggatttga acccacgtaa
      481 ggcggatcta gagtccgctg cctaacccct agactacccc ggcgaattca gcgttacaag
      541 tattacacaa agttttttat gttgagaata tttttttgat ggggcgccac ttatttttga
      601 tcgttcgctc aaagaagcgc ggccgctttc accgtcatca ccgaaacgcg cgaggcagca
      661 gatcaattcg cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat cgaatggtgc
      721 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
      781 gtgaaaccag taacgttata cgatgtcgca gagtatgcc
//
