LOCUS Exported 2640 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION systhesis of vioE gene in the violacein pathway. ACCESSION . VERSION . KEYWORDS pKMV-VioE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2640) AUTHORS Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J TITLE YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. JOURNAL Nucleic Acids Res. 2015 May 8. pii: gkv464. PUBMED 25956650 REFERENCE 2 (bases 1 to 2640) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2640 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 50..68 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind 77..94 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 78..94 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(744..760) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(744..760) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(772..789) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(943..1531) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1023..1042) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1702..2517) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 2425..2444 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" promoter complement(2518..2622) /gene="bla" /label=AmpR promoter ORIGIN 1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 61 cacgaggccc tttcgttgta aaacgacggc cagtcgaacc acgcaatgcg tctcgatccg 121 cagtgtcttg cgtctctgat ggaaaacaga gaaccaccat tgttgccagc tagatggtct 181 tctgcttacg tttcttactg gagtccaatg ttgccagacg accaattgac ctctggttac 241 tgttggttcg actacgaaag agacatctgt agaatcgacg gtttgttcaa cccgtggtct 301 gaaagagaca ccggttacag attgtggatg tctgaagttg gtaacgctgc ttctggtaga 361 acctggaagc aaaaggttgc ttacggtaga gaaagaaccg ctttgggtga acaattgtgt 421 gaaagaccat tggacgacga aaccggtcca ttcgctgaat tgttcttgcc acgcgacgtt 481 ttgagaagat tgggtgctag acacatcggt agaagagttg ttttgggtag agaagctgac 541 ggttggagat accaaagacc aggtaagggt ccatctacct tgtacttgga cgctgcttct 601 ggtaccccat tgagaatggt taccggtgac gaggctagta gagcttcttt gagagacttc 661 ccaaacgttt ctgaagctga aatcccagac gctgttttcg ctgctaagag atagcagaga 721 cggagtcact gccaaccgag acggtcatag ctgtttcctg tgtgccgctt cctcgctcac 781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 841 aatacggtta cccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 961 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 1021 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 1081 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 1141 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 1201 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 1261 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 1321 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 1381 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 1441 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 1501 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 1561 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 1621 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 1681 tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt 1741 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga 1801 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac 1861 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga 1921 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt 1981 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa 2041 accgttattc attcgtgatt gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg 2101 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac acggccagcg catcaacaat 2161 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc 2221 tgtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg 2281 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct 2341 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat 2401 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc 2461 catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcatact 2521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 2581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt //