LOCUS       Exported                 854 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    GPER-Tango
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg gacgtaacat cccaagcaag aggagtgggt cttgagatgt
      301 acccggggac tgcgcagccc gcagctccta acactacttc acccgagctg aacctgtcac
      361 accctctcct gggcactgcg ctcgcgaacg gaaccgggga gctgtctgag caccagcagt
      421 acgtcatagg cctgttcctg tcctgtttgt ataccatttt tctcttcccc attgggtttg
      481 tggggaacat cctgattctt gttgttaaca tcagcttcag ggagaaaatg acgattcctg
      541 acttgtactt tatcaacctg gccgtcgccg acttgatttt ggtggcggat tctctgattg
      601 aggtttttaa cctccacgag agatattacg acatcgctgt gctgtgtacc tttatgagcc
      661 tctttctgca ggttaacatg tattcctcag tgttttttct gacctggatg tcattcgaca
      721 gatacatcgc cctcgccagg gctatgaggt gctctctgtt ccgcacgaaa caccacgccc
      781 ggcttagttg cgggctcatt tggatggcct cagtgagcgc aacactggtg ccgtttactg
      841 ccgtacacct ccag
//
