LOCUS Exported 12080 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pdestRG5271Neo SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12080) AUTHORS Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D TITLE An antibiotic selection marker for nematode transgenesis. JOURNAL Nat Methods. 2010 Aug 22. ():. PUBMED 20729841 REFERENCE 2 (bases 1 to 12080) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12080 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 796..1590 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(850..869) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 1460..1479 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 2291..2307 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 2292..2308 /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter complement(3687..3705) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3726..3742) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3726..3742) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3739..3761) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3750..3766 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3774..3804) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3819..3840 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3957..3974) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4128..4716) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4208..4227) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4887..5747) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5510..5529 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5748..5852) /gene="bla" /label=AmpR promoter rep_origin complement(5878..6333) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6015..6036) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6227..6246 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6460..6482 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6474..6491 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 6475..6491 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 6498..6517 /label=T7 /note="T7 promoter, forward primer" promoter 6498..6516 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 8038..8062 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(8994..9014) /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" primer_bind 9762..9790 /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" CDS complement(9833..9853) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind complement(9862..9961) /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 10118..10220 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 10221..10880 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(10287..10306) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 11222..11527 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 11406..11425 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(11917..12041) /gene="mutant version of attR" /label=attR4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" ORIGIN 1 catttctaaa ttttagttta ataaaaaaat tttaaaataa tacgagtttt gagactaact 61 ttcaataaaa accaaaaaat tgcttcaaaa aagagctcgc gccgatttac gataaacaca 121 gcgcgctacg acactccgaa aaccgcgccg caccattaca ttctccgccc caccgcacgg 181 tttgcggaag gcgatgaaat tgctgggcct aaaattttaa cccttttttt cccgtttttc 241 aactgttttc ccgatttttt cgatattttt ttcaggtttt tagttagaaa agtatataaa 301 aattgcatta tttgtagttt tcgctcgtaa tttgtgatag aaaacggcca atttgcctag 361 atttgtgatt ctaccaagtg gaatttttgg gaaaatatca atattccacg atttttattc 421 aaatcataac gtttcgaaat tatcagctta aataaaacta aaataaataa taaaatacta 481 aaaaccctga atttctcggt tctcggttca gcttgagttc gttctataca cgttatcgat 541 ttttgtcatt tcgtttaatc atgggttttg attcatttta aatatgaaat ttgatttatt 601 cctcgtttcg aagttggttt gtcttgtccg gccggtaaac aaatgttatt aaaaaaaata 661 ataaatttcg aaatttttcg taatgttccg caatcgtgtt tttccgttta tctcgtcttc 721 ccttccttca aatatactat ttattttctc aattcacata ttttcagcca gccctgctca 781 acaagtggaa taaaaatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 841 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 901 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 961 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 1021 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 1081 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 1141 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 1201 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 1261 gatgatctgg acgaagagca tcaggggctc gcaccagccg aactgttcgc caggctcaag 1321 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 1381 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 1441 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 1501 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 1561 ttctatcgcc ttcttgacga gttcttctga gtccaattac tcttcaacat ccctacatgc 1621 tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt 1681 ctttgttttt tagcttcttt taagccacct ctaacaatga aattgtgtag attcaaaaat 1741 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa 1801 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata 1861 aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt 1921 tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc 1981 atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta 2041 tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg 2101 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg 2161 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg 2221 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca 2281 taactgtttc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg 2341 tatatatata ttgggatgca ctctcaatgg tcctgtaaca cttttttgtg tgttagtgac 2401 cagtgctccg ctcccagtag atgtttttgc acaaaaacat accaaaatgt tttaccatat 2461 tcatagatca ttgtcataga aatttgttta attctgaaaa gtttcctctg ttttatggca 2521 gaaacaattc attgagataa tctgacttat tattcattgg gggtaagtgc tctatgggat 2581 ccctttaatc gacgattaac cttcaaacct caaaaaaaaa aaacaagaaa atcacaaagc 2641 ttttcctatc ttcacaaagc ttttcctatc ttaaaggcat aactgtgtgt gtatatctga 2701 tctttgtcca actgtataga tgtgttttgt ttctgagaac aaaagttgtc catggaccaa 2761 atttctatag aagttgcacg cactgtactt ctagatttta ggtagagtgt gtgattgaaa 2821 gttatattca gcaaaattat aatcagtgat cctttccaaa gcttggcatt gttgcttgaa 2881 atcctgttca agtagtgttt ggcaaaaaat tcccaaaatt tccgatatgt actctatgtt 2941 taattttggg ttttaaataa atacgttgaa ttacgcaagc tgttacttct aaaaaacatt 3001 gtatacattt tagggttgta gtgaactttt ataatatttg ctcaaaaact ctgaaacatt 3061 aaaatgacgt gctggtctgc ttttattctc gaattatatg atcaaatctc tgattttctg 3121 gttaaaaaat tccctattgt attttgatag actttttctc cttatttcat tttttcacag 3181 ggtatatgag atgatgttca aagtggttca taatatccag ttaagaatca aaatacttca 3241 ctttcaaatt aagtgtcctg ggcttctgaa tgcaggtgtt gtctacatat tatttctcaa 3301 atcaaaatac tctgtcattt cttacgagct taaccaattc accttttgta tggcagcagc 3361 aggcgtcata catttccatc tcaattgacc tattctcgag tctcggctgt ccatgtaatt 3421 atccattttc accgtgaacc cttctgtcca tcgtattttt ttcccatcgc tcctctcttt 3481 ctcttcatct ccacagtttg acctttacac agaaactctt tttcccgaat aatatgtagt 3541 tcttctcgta gttttcggaa gaagtaccgc ccgccgcaag gatttctctt cgttccgtcc 3601 atcttaaaaa cacgagctta attgagaagc gatcatgtct tcttgtcccg gcggccgcca 3661 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa 3721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 3781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 3841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 3901 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 3961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 4081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 4141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 4201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 4261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 4321 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 4381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 4441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 4501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 4561 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 4621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 4681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 4741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 4801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 4861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 4921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 4981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 5101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 5161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 5221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 5281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 5341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 5401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 5461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 5521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 5581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 5641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 5701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 5761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 5821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa 5881 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 5941 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 6001 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 6061 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 6121 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 6181 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 6241 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 6301 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact 6361 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 6421 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 6481 cgacggccag tgaattgtaa tacgactcac tatagggcga attgggtacc aattttcata 6541 ccttaaactc ctcaaagaaa ccaataatta tttgaacaaa acacacaaaa aagtaccagg 6601 tttttcaaaa ctttcaatcg aatattttgg ttaatttcca aatttcggaa caatttgaag 6661 cattgctttg tgctctccta tgatattatt cttcacttat cttgcatggc attttcactg 6721 taatccctat atgtcacttt cacatccatc agttttttta aacattgaat taatatgttt 6781 ctagtactta actacatgaa ctagttgacc tactctctgt ggaaaaaagg acaacagcaa 6841 gtagaatggg aggaagtttc tctttttctt attttcattg aaaagggtga aaacgaggtt 6901 gttgctccat ttccgactgt cgtcaagact gtacctacag gataattacg caatgttgtt 6961 ctcccgcaca gcaaaaaaaa agagaaaagt cgaatggttt gtgtgacacc ttgagatgcc 7021 gcatgatcga tgaaccttca ctcaaataaa ccgaaaagta aattatcgaa atgaaaggag 7081 cgagcaaaaa aaagaaatag agggcagttc aacgaaaact ttccacttct tttgatctat 7141 tatattgcag gtatcaaaat caggatttaa gggaaatgtg ttctgtgctt ccgatatact 7201 gagcgttcat ggattaagaa gacaatattg agaaggaatc agctagattt tccgatatca 7261 aaatttcaga ccaaacaaaa attgaaaaaa aaatcgtaat tccattaata tacttcttgt 7321 agatcttata tataggtagg aataaagacc aacttatgtg cctttaagca ggcgtaactc 7381 gctttcttgg cgtttggggg caacttactg cacaacatca attactttct accttcagcc 7441 tgacaactta ttaaaagctg atcaaataaa ttggtagtcc tggcaagaaa cggccaacca 7501 aaaatgttta acacgaattt ttcaaatttg gcaaaagctt tcactcaact tctaagctta 7561 catcaccgta tagaaagata tcacacggac tggttaggtc tttttcaagc cgtttaggcg 7621 agtaggcagg tattttgtgc ctacagtgcg tgcaacttct atagaacccc ctaaattttc 7681 gagtttcagc atgttctgat atttttcaag aaaatttgtt aatgtcaatc gattctaaat 7741 gccaaaaaac ctactgtcca aaatttttag aaaaatatat gcaaaactac gagaattagg 7801 ccaaaaagtc gtaaaaacga ccgtgcaact tctatagaac ccctcagaat tttgatttta 7861 atcgcaaaaa gcactgaatt tgtttaaatt tttcaaaata attttgtaaa acaataacat 7921 caaaatgatc taaatactta gttcgttcat taaaaacatg aaaaatgcta cgtggaaata 7981 tatatatata tatagggccc aaagctggta ccggtccgga attcccgggt cgagatatca 8041 actttgtaca agaaagctgg gttacggccg actagtagga aacagttatg tttggtatat 8101 tgggaatgta ttctgtcatt taaggcaaaa accccataga cactactcca ctttcaaatt 8161 atcaacttct actcaccttc cactgagcct caaacccaaa ccttcttccg atctttcttg 8221 catcgtgctc atcaatactt gtgattttag caagaaaaac gccgtcctcg aaactcttga 8281 caaacaatag gggaaacccc caaaaagcaa aagcaggaaa attaatttca taaactttca 8341 cttgattgaa aaattccaaa aatactccaa aactttttca cgatgagcat gatttgacgt 8401 catgagaggc ccagacgtgc gaagaaataa aaattgcggt cataaactga aacgtaacat 8461 atgataaggt attttgtgtg cggttttttc tatgatgttt caaaaaaaat ttatcagaag 8521 taaaaaaaac ataagaagtg tacacagaac attgtgtaga ttttgggata gaattattat 8581 taatgggggg agggagcaca atttttttcg actttttatt acgaattaat tctatttttg 8641 aatctacaca atttcattgt tagaggtgac ttaaaagaag ctaaaaaaca aagaaattaa 8701 gaagaagttt ttttgataat aacaaaaata gggggtggga gcacagggag aaagagcatg 8761 tagggatgtt gaagagtaat tggacttaga agtcagaggc acgggcgcga gatgctgaaa 8821 atttaaatat tactcgttag ttagtagaac tcagtttaaa cttactctga tgacagcggc 8881 ccctattatt tttgacacca gacaagttgg taatggtagc gaccggcgct cagttggaat 8941 tctacgaatg ctatttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa 9001 actcaagaag gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg 9061 tggacaggta atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt 9121 gataatggtc tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttct 9181 gaaaatttaa atcagattag ttagttagta aaatcatgtt taaacttaca actttgattc 9241 cattcttttg tttgtctgcc atgatgtata cattgtgtga gttatagttg tattccaatt 9301 tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ttttaactcg attctattaa 9361 caagggtatc accttcaaac ttgacttcag cacctgaaaa tttaaatatg tatggttagt 9421 tagtaccgaa ctgtttaaac ttacgtgtct tgtagttccc gtcatctttg aaaaatatag 9481 ttctttcctg tacataacct tcgggcatgg cactcttgaa aaagtcatgc cgtttcatat 9541 gatctgggta tctcgagaag cattgaacac cataacagaa agtagtgaca agtgttggct 9601 gaaaatttaa ataatcaggg ttagttagta tatatatgtt taaacttacc catggaacag 9661 gtagttttcc agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt gcatcacctt 9721 caccctctcc actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa 9781 ttgggacaac tccagtgaaa agttcttctc ctttactcat tttttctacc ggtaccttac 9841 gcttcttctt tggagcagtc aagcctgctt ttttgtacaa agttggcatt ataaaaaagc 9901 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt 9961 ggagctctag agcgtcgact aagttggcag catcacccga cgcactttgc gccgaataaa 10021 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg 10081 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca 10141 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt 10201 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 10261 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 10321 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 10381 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc 10441 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 10501 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 10561 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 10621 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 10681 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 10741 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 10801 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 10861 agtggcaggg cggggcgtaa tcgcgtggat ccggcttact aaaagccaga taacagtatg 10921 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta 10981 tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta 11041 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag 11101 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct 11161 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag ggactggtga 11221 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt 11281 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg 11341 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag 11401 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt 11461 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg 11521 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgataca gtagaaatta 11581 cagaaacttt atcacgttta gtaagtatag aggctgaaaa tccagatgaa gccgaacgac 11641 ttgtaagaga aaagtataag agttgtgaaa ttgttcttga tgcagatgat tttcaggact 11701 atgacactag cgtatatgaa taggtagatg tttttatttt gtcacacaaa aaagaggctc 11761 gcacctcttt ttcttatttc tttttatgat ttaatacggc attgaggaca atagcgagta 11821 ggctggatac gacgattccg tttgagaaga acatttggaa ggctgtcggt cgagctcgaa 11881 ttctacaggt cactaatacc atctaagtag ttgattcata gtgactgcat atgttgtgtt 11941 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata 12001 tcattttacg tttctcgttc aacttttcta tacaaagttg atatcactag tcggcggccg 12061 ctctagagga tccaagggcc //