LOCUS Exported 3918 bp ds-DNA linear SYN 19-JUN-2021 DEFINITION Expresses humanized PcCpf1. ACCESSION . VERSION . KEYWORDS pY017 (pcDNA3.1-hPcCpf1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3918) AUTHORS Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F TITLE Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. JOURNAL Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. PUBMED 26422227 REFERENCE 2 (bases 1 to 3918) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3918 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 3781..3828 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 3835..3915 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA" ORIGIN 1 atggacagcc tgaaggattt caccaacctg taccccgtgt ccaagacact gcggtttgag 61 ctgaagcctg tgggcaagac cctggagaat atcgagaagg ccggcatcct gaaggaggat 121 gagcacagag ccgagagcta ccggagagtg aagaagatca tcgatacata tcacaaggtg 181 ttcatcgaca gctccctgga gaacatggcc aagatgggca tcgagaatga gatcaaggcc 241 atgctgcagt ccttttgcga gctgtataag aaggaccaca ggaccgaggg agaggacaag 301 gccctggata agatcagggc cgtgctgagg ggcctgatcg tgggagcctt caccggcgtg 361 tgcggccggc gggagaacac agtgcagaat gagaagtatg agagcctgtt taaggagaag 421 ctgatcaagg agatcctgcc agatttcgtg ctgtctacag aggccgagtc cctgcccttt 481 tctgtggagg aggccaccag aagcctgaag gagttcgact cctttacatc ttacttcgcc 541 ggcttttatg agaaccggaa gaatatctac tctaccaagc cccagagcac agccatcgcc 601 tatagactga tccacgagaa cctgcctaag ttcatcgata atatcctggt gtttcagaag 661 atcaaggagc caatcgccaa ggagctggag cacatcaggg cagacttcag cgccggcggc 721 tacatcaaga aggatgagcg cctggaggac atcttttccc tgaactacta tatccacgtg 781 ctgtctcagg ccggcatcga gaagtacaat gccctgatcg gcaagatcgt gaccgagggc 841 gatggcgaga tgaagggcct gaacgagcac atcaacctgt ataatcagca gaggggccgc 901 gaggaccggc tgccactgtt cagacccctg tataagcaga tcctgtctga tagggagcag 961 ctgtcctatc tgccagagtc tttcgagaag gacgaggagc tgctgagggc cctgaaggag 1021 ttttacgatc acatcgcaga ggacatcctg ggaaggaccc agcagctgat gacaagcatc 1081 tccgagtacg atctgtcccg gatctatgtg agaaacgata gccagctgac cgacatctcc 1141 aagaagatgc tgggcgattg gaatgccatc tacatggccc gggagagagc ctatgaccac 1201 gagcaggccc ccaagcgcat cacagccaag tacgagaggg accgcatcaa ggccctgaag 1261 ggcgaggagt ctatcagcct ggccaacctg aacagctgca tcgccttcct ggacaacgtg 1321 agggattgtc gcgtggacac ctatctgtct acactgggac agaaggaggg acctcacggc 1381 ctgagcaacc tggtggagaa cgtgttcgcc tcctaccacg aggccgagca gctgctgtct 1441 tttccctatc ctgaggagaa caatctgatc caggacaagg ataacgtggt gctgatcaag 1501 aacctgctgg ataatatcag cgacctgcag aggttcctga agccactgtg gggcatgggc 1561 gatgagcccg acaaggatga gaggttttac ggcgagtaca attatatcag gggcgccctg 1621 gaccaggtca tccctctgta taacaaggtg cggaattatc tgacccgcaa gccatactcc 1681 acacgcaagg tgaagctgaa cttcggcaat agccagctgc tgtccggctg ggataggaac 1741 aaggagaagg acaattcttg cgtgatcctg cgcaagggcc agaacttcta cctggccatc 1801 atgaacaatc ggcacaagcg gagcttcgag aataagatgc tgcccgagta taaggagggc 1861 gagccttact tcgagaagat ggattataag tttctgccag accccaacaa gatgctgccc 1921 aaggtgttcc tgtctaagaa gggcatcgag atctacaagc ctagcccaaa gctgctggag 1981 cagtatggcc acggcaccca caagaagggc gataccttca gcatggacga tctgcacgag 2041 ctgatcgact tctttaagca ctccatcgag gcccacgagg attggaagca gttcggcttt 2101 aagttcagcg acaccgccac atacgagaac gtgagcagct tctaccggga ggtggaggac 2161 cagggctaca agctgtcttt tagaaaggtg tccgagtctt acgtgtatag cctgatcgat 2221 cagggcaagc tgtacctgtt ccagatctat aacaaggact ttagcccttg ttccaagggc 2281 accccaaatc tgcacacact gtactggcgg atgctgttcg atgagagaaa cctggccgac 2341 gtgatctata agctggatgg caaggccgag atcttctttc gggagaagtc cctgaagaat 2401 gaccacccaa cccaccctgc aggcaagccc atcaagaaga agagccggca gaagaagggc 2461 gaggagagcc tgttcgagta cgatctggtg aaggaccgga gatataccat ggataagttt 2521 cagttccacg tgccaatcac aatgaacttt aagtgctctg ccggcagcaa ggtgaacgac 2581 atggtgaatg cccacatcag ggaggccaag gacatgcacg tgatcggcat cgataggggc 2641 gagcgcaatc tgctgtatat ctgcgtgatc gacagccgcg gcaccatcct ggatcagatc 2701 tccctgaaca caatcaatga catcgattat cacgatctgc tggagtccag ggacaaggat 2761 cgccagcagg agcacaggaa ctggcagacc atcgagggca tcaaggagct gaagcagggc 2821 tacctgtctc aggccgtgca ccgcatcgcc gagctgatgg tggcctataa ggccgtggtg 2881 gccctggagg acctgaacat gggcttcaag cggggcagac agaaggtgga gagcagcgtg 2941 taccagcagt ttgagaagca gctgatcgac aagctgaatt atctggtgga taagaagaag 3001 cggcccgagg acatcggagg cctgctgaga gcctaccagt tcaccgcccc tttcaagagc 3061 tttaaggaga tgggcaagca gaacggcttt ctgttctata tccctgcctg gaacacatcc 3121 aatatcgacc caaccacagg cttcgtgaac ctgtttcacg tgcagtacga gaatgtggat 3181 aaggccaaga gcttctttca gaagttcgac agcatctcct acaaccctaa gaaggattgg 3241 tttgagttcg cctttgacta taagaacttc accaagaagg ccgagggctc taggagcatg 3301 tggattctgt gcacccacgg ctcccggatc aagaacttca gaaattctca gaagaatggc 3361 cagtgggata gcgaggagtt tgccctgacc gaggccttca agtccctgtt tgtgcggtac 3421 gagatcgatt ataccgccga cctgaaaacc gccatcgtgg acgagaagca gaaggatttc 3481 tttgtggacc tgctgaagct gttcaagctg accgtgcaga tgagaaactc ctggaaggag 3541 aaggacctgg attacctgat ctctccagtg gccggcgccg atggcaggtt ctttgacaca 3601 cgcgagggca ataagagcct gcccaaggac gcagatgcaa acggagccta taatatcgcc 3661 ctgaagggcc tgtgggcact gaggcagatc agacagacct ccgagggcgg caagctgaag 3721 ctggccatct ctaacaagga gtggctgcag tttgtgcagg agagatccta cgagaaggac 3781 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg atcctaccca 3841 tacgatgttc cagattacgc ttatccctac gacgtgcctg attatgcata cccatatgat 3901 gtccccgact atgcctaa //