LOCUS Exported 4107 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION Expresses humanized PdCpf1. ACCESSION . VERSION . KEYWORDS pY018 (pcDNA3.1-hPdCpf1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4107) AUTHORS Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F TITLE Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. JOURNAL Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. PUBMED 26422227 REFERENCE 2 (bases 1 to 4107) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4107 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 3970..4017 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 4024..4104 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA" ORIGIN 1 atggagaact atcaggagtt caccaacctg tttcagctga ataagacact gagattcgag 61 ctgaagccca tcggcaagac ctgcgagctg ctggaggagg gcaagatctt cgccagcggc 121 tcctttctgg agaaggacaa ggtgagggcc gataacgtga gctacgtgaa gaaggagatc 181 gacaagaagc acaagatctt tatcgaggag acactgagct ccttctctat cagcaacgat 241 ctgctgaagc agtactttga ctgctataat gagctgaagg ccttcaagaa ggactgtaag 301 agcgatgagg aggaggtgaa gaaaaccgcc ctgcgcaaca agtgtacctc catccagagg 361 gccatgcgcg aggccatctc tcaggccttt ctgaagagcc cccagaagaa gctgctggcc 421 atcaagaacc tgatcgagaa cgtgttcaag gccgacgaga atgtgcagca cttctccgag 481 tttaccagct atttctccgg ctttgagaca aacagagaga atttctactc tgacgaggag 541 aagtccacat ctatcgccta taggctggtg cacgataacc tgcctatctt catcaagaac 601 atctacatct tcgagaagct gaaggagcag ttcgacgcca agaccctgag cgagatcttc 661 gagaactaca agctgtatgt ggccggctct agcctggatg aggtgttctc cctggagtac 721 tttaacaata ccctgacaca gaagggcatc gacaactata atgccgtgat cggcaagatc 781 gtgaaggagg ataagcagga gatccagggc ctgaacgagc acatcaacct gtataatcag 841 aagcacaagg accggagact gcccttcttt atctccctga agaagcagat cctgtccgat 901 cgggaggccc tgtcttggct gcctgacatg ttcaagaatg attctgaagt gatcaaggcc 961 ctgaagggct tctacatcga ggacggcttt gagaacaatg tgctgacacc tctggccacc 1021 ctgctgtcct ctctggataa gtacaacctg aatggcatct ttatccgcaa caatgaggcc 1081 ctgagctccc tgtcccagaa cgtgtatcgg aatttttcta tcgacgaggc catcgatgcc 1141 aacgccgagc tgcagacctt caacaattac gagctgatcg ccaatgccct gcgcgccaag 1201 atcaagaagg agacaaagca gggccggaag tctttcgaga agtacgagga gtatatcgat 1261 aagaaggtga aggccatcga cagcctgtcc atccaggaga tcaacgagct ggtggagaat 1321 tacgtgagcg agtttaactc taatagcggc aacatgccaa gaaaggtgga ggactacttc 1381 agcctgatga ggaagggcga cttcggctcc aacgatctga tcgaaaatat caagaccaag 1441 ctgagcgccg cagagaagct gctgggcaca aagtaccagg agacagccaa ggacatcttc 1501 aagaaggatg agaactccaa gctgatcaag gagctgctgg acgccaccaa gcagttccag 1561 cactttatca agccactgct gggcacaggc gaggaggcag atcgggacct ggtgttctac 1621 ggcgattttc tgcccctgta tgagaagttt gaggagctga ccctgctgta taacaaggtg 1681 cggaatagac tgacacagaa gccctattcc aaggacaaga tccgcctgtg cttcaacaag 1741 cctaagctga tgacaggctg ggtggattcc aagaccgaga agtctgacaa cggcacacag 1801 tacggcggct atctgtttcg gaagaagaat gagatcggcg agtacgatta ttttctgggc 1861 atctctagca aggcccagct gttcagaaag aacgaggccg tgatcggcga ctacgagagg 1921 ctggattact atcagccaaa ggccaatacc atctacggct ctgcctatga gggcgagaac 1981 agctacaagg aggacaagaa gcggctgaac aaagtgatca tcgcctatat cgagcagatc 2041 aagcagacaa acatcaagaa gtctatcatc gagtccatct ctaagtatcc taatatcagc 2101 gacgatgaca aggtgacccc atcctctctg ctggagaaga tcaagaaggt gtctatcgac 2161 agctacaacg gcatcctgtc cttcaagtct tttcagagcg tgaacaagga agtgatcgat 2221 aacctgctga aaaccatcag ccccctgaag aacaaggccg agtttctgga cctgatcaat 2281 aaggattatc agatcttcac cgaggtgcag gccgtgatcg acgagatctg caagcagaaa 2341 accttcatct actttccaat ctccaacgtg gagctggaga aggagatggg cgataaggac 2401 aagcccctgt gcctgttcca gatcagcaat aaggatctgt ccttcgccaa gacctttagc 2461 gccaacctgc ggaagaagag aggcgccgag aatctgcaca caatgctgtt taaggccctg 2521 atggagggca accaggataa tctggacctg ggctctggcg ccatcttcta cagagccaag 2581 agcctggacg gcaacaagcc cacacaccct gccaatgagg ccatcaagtg taggaacgtg 2641 gccaataagg ataaggtgtc cctgttcacc tacgacatct ataagaacag gcgctacatg 2701 gagaataagt tcctgtttca cctgagcatc gtgcagaact ataaggccgc caatgactcc 2761 gcccagctga acagctccgc caccgagtat atcagaaagg ccgatgacct gcacatcatc 2821 ggcatcgata ggggcgagcg caatctgctg tactattccg tgatcgatat gaagggcaac 2881 atcgtggagc aggactctct gaatatcatc aggaacaatg acctggagac agattaccac 2941 gacctgctgg ataagaggga gaaggagcgc aaggccaacc ggcagaattg ggaggccgtg 3001 gagggcatca aggacctgaa gaagggctac ctgagccagg ccgtgcacca gatcgcccag 3061 ctgatgctga agtataacgc catcatcgcc ctggaggatc tgggccagat gtttgtgacc 3121 cgcggccaga agatcgagaa ggccgtgtac cagcagttcg agaagagcct ggtggataag 3181 ctgtcctacc tggtggacaa gaagcggcct tataatgagc tgggcggcat cctgaaggcc 3241 taccagctgg cctctagcat caccaagaac aattctgaca agcagaacgg cttcctgttt 3301 tatgtgccag cctggaatac aagcaagatc gatcccgtga ccggctttac agacctgctg 3361 cggcccaagg ccatgaccat caaggaggcc caggacttct ttggcgcctt cgataacatc 3421 tcttacaatg acaagggcta tttcgagttt gagacaaact acgacaagtt taagatcaga 3481 atgaagagcg cccagaccag gtggacaatc tgcaccttcg gcaatcggat caagagaaag 3541 aaggataaga actactggaa ttatgaggag gtggagctga ccgaggagtt caagaagctg 3601 tttaaggaca gcaacatcga ttacgagaac tgtaatctga aggaggagat ccagaacaag 3661 gacaatcgca agttctttga tgacctgatc aagctgctgc agctgacact gcagatgcgg 3721 aactccgatg acaagggcaa tgattatatc atctctcctg tggccaacgc cgagggccag 3781 ttctttgact cccgcaatgg cgataagaag ctgccactgg atgcagacgc aaacggagcc 3841 tacaatatcg cccgcaaggg cctgtggaac atccggcaga tcaagcagac caagaacgac 3901 aagaagctga atctgagcat ctcctctaca gagtggctgg atttcgtgcg ggagaagcct 3961 tacctgaaga aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga 4021 tcctacccat acgatgttcc agattacgct tatccctacg acgtgcctga ttatgcatac 4081 ccatatgatg tccccgacta tgcctaa //