LOCUS       Exported                9672 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDD04neo pmyo2::gfp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Aug 22. ():.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2226..2242
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2226..2242
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2276..2292)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2277..2293)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(3001..3795)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3112..3131)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3722..3741
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     protein_bind    4632..4652
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7165..7189
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7214..7242)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7990..8010
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(8942..8966)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9028..9047)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9029..9047)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      9215..9670
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9302..9321)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9512..9533
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccgctctag aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata
     2281 ccgtcgacct cgagaaacag ttatgtttgg tatattggga atgtattctg tcatttaagg
     2341 caaaaacccc atagacacta ctccactttc aaattatcaa cttctactca ccttccactg
     2401 agcctcaaac ccaaaccttc ttccgatctt tcttgcatcg tgctcatcaa tacttgtgat
     2461 tttagcaaga aaaacgccgt cctcgaaact cttgacaaac aataggggaa acccccaaaa
     2521 agcaaaagca ggaaaattaa tttcataaac tttcacttga ttgaaaaatt ccaaaaatac
     2581 tccaaaactt tttcacgatg agcatgattt gacgtcatga gaggcccaga cgtgcgaaga
     2641 aataaaaatt gcggtcataa actgaaacgt aacatatgat aaggtatttt gtgtgcggtt
     2701 ttttctatga tgtttcaaaa aaaatttatc agaagtaaaa aaaacataag aagtgtacac
     2761 agaacattgt gtagattttg ggatagaatt attattaatg gggggaggga gcacaatttt
     2821 tttcgacttt ttattacgaa ttaattctat ttttgaatct acacaatttc attgttagag
     2881 gtgacttaaa agaagctaaa aaacaaagaa attaagaaga agtttttttg ataataacaa
     2941 aaataggggg tgggagcaca gggagaaaga gcatgtaggg atgttgaaga gtaattggac
     3001 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     3061 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     3121 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     3181 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     3241 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     3301 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     3361 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     3421 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     3481 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     3541 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     3601 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     3661 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     3721 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     3781 atcttgttca atcattttta ttccacttgt tgagcggggc tggctgaaaa tatgtgaatt
     3841 gagaaaataa atagtatatt tgaaagaagg gaagacgaga taaacggaaa aacacgattg
     3901 cggaacatta cgaaaaattt cgaaatttat tattttttta ataacatttg tttaccggcc
     3961 ggacaagaca aaccaacttc gaaacgagga ataaatcaaa tttcatattt aaaatgaatc
     4021 aaaacccatg attaaacgaa atgacaaaaa tcgataacgt gtatagaacg aactcaagct
     4081 gaaccgagaa ccgagaaatt cagggttttt agtattttat tatttatttt agttttattt
     4141 aagctgataa tttcgaaacg ttatgatttg aataaaaatc gtggaatatt gatattttcc
     4201 caaaaattcc acttggtaga atcacaaatc taggcaaatt ggccgttttc tatcacaaat
     4261 tacgagcgaa aactacaaat aatgcaattt ttatatactt ttctaactaa aaacctgaaa
     4321 aaaatatcga aaaaatcggg aaaacagttg aaaaacggga aaaaaagggt taaaatttta
     4381 ggcccagcaa tttcatcgcc ttccgcaaac cgtgcggtgg ggcggagaat gtaatggtgc
     4441 ggcgcggttt tcggagtgtc gtagcgcgct gtgtttatcg taaatcggcg cgaactcttt
     4501 tttgaagcaa ttttttttgg tttttattga aagttagtct caaaactcgt attattttaa
     4561 aattttttta ttaaactaaa atttagaaat gggcccttgg atcctctaga gcggccgccg
     4621 actagtgata tcaactttgt atagaaaagt tgaaggtctc ccagaacgtg aataaaagct
     4681 gctcggacgt tgatgttatc gccgttgcca ccaccatgag agtgaccatg agagcttccg
     4741 cctccgtgag aatgtgaatg accaccaaag tacaacaatg caagcatgct taaattttga
     4801 ttttgaatat caattaagat acatatgtca cattagattt gtttcgaact caaaaaaatt
     4861 tctttgaaaa ccgtaagtcc gtgacagcct aatttttagt taaaagaaaa taacatggac
     4921 agacattttt ctgacatgtc aacattcctg gggctcccac ccggataaaa gttcatgcca
     4981 aatatcgatt tcaaatcgaa gtaaaaaagt gtataccagt taataaagct atcagactcg
     5041 tttcataaaa acacttacac caagtttaca accactccaa gagcggctgt caaagccata
     5101 attcctcctt ctacttcata atctccgctg acaattctca taattgcgag aacaactaat
     5161 actccagtga ctatccaaat aaggaaaact gagaaaaatg ctcccaaaac ttctgaaatt
     5221 catttcaaac tatgtcgctc aattcttcca actacccacc ggctctatga aacccaaagc
     5281 tcattttttg cgatggtggc cgtcttgcaa tgtacagaga aaacagcgaa atgagcactg
     5341 acgcaaaatc agtgagcaag tgtgcggcat ctgtgacaat tgcgagtgag cccgcgagaa
     5401 caccaccaat aacttcgcaa accataaaaa ataggcagag cacgacggtg agccataaaa
     5461 ttcgcgttgc tgaaacaaat agtttgaaga aggtttcatt aggactcgag tttggcagaa
     5521 attttaaaag caatacctct ccgattgcta tcatgtgaat ctgttgagtc agcttcatcg
     5581 tggcaatgaa agttatcata ggtgactgtg gtttttctac cttcgtctgt attataaatg
     5641 tgaggaactc tgtaatacaa aaagatctca tgttttttct ttggtaatac gtcatagaag
     5701 cacaggaaat cacaactctg atataaacga acatcaacta catcacaaaa ctaacccatt
     5761 ttcaagtagt ggtgctcgtt cagaaatcat tatggattat ggagaaaagg aaaccgatta
     5821 ggccaatcga ttttgttttg atagaattga agacgcagaa taattgtgtt gtgtatagtg
     5881 tacgagaaaa tggagcaact tgtatatggg cactgttatc attattgaat agcgtgcgga
     5941 ggtttagaga aggaagttgt gtgttatcaa tcataaacta gaacatgtct cgaggcattt
     6001 gaattggggg tggtggacag taactgtctg taataataat tactcctgac caggttgcaa
     6061 ttcgagtttt gataagcata attatacctt gtacattgtg ggttttgtgc tgtggacgtt
     6121 ttattgtgac atccccataa gctacaagaa caaaaatgaa atgtaaaagt attgaaaacg
     6181 tcgtacattt tatatctgag tagtatcctt tgctttaaat gtccataaaa ctaattttat
     6241 aatcaataaa acaacgtttg taaatcaact gagtttacaa gtagagacat tgagggatac
     6301 tttcactatg ctaaagtgat aatcgaccaa ataataactt cactttggta tttattcctg
     6361 tcttataaat gttatgtatg aattaaattc atatgcatat ggctcactct gacaattttt
     6421 aataatcttc cagatcaata ttgactaccg atgcgggtgg tcttttgctt tgaattctgc
     6481 tgaactttac accccgaaca gcaatgtgtg cttcagccta aaaaaaagta agtgtgttaa
     6541 tcagtgcccc cgattcttca ttttttgccc ctctctcccg tttcgtcggc aaaagaagag
     6601 aaaataaaga taagtctcaa gataggttgg taatcgctaa agtggttgtg tggataagag
     6661 tagcaaaatg gcaggaagag cactttgcgc gcacacactg tactcattgt tctggataaa
     6721 attctctcgt tgtttgccgt cggatgtctg cctctctgca ttgagccggc ttcttcacta
     6781 tctttagtta acctaaaatg ccgtttcttt tctcgtatcc ccactatccc gttgaggttc
     6841 tctgctctct tcgctcccta ccgccagcga gcaactatcc gtgggggcgc cttgctcgga
     6901 agatgggggg gaagaaagaa gatttttgct atttgcactt gagaaagaga cttttcctgc
     6961 gtcgatggtt agagaacagt gtgcagacac ttttcagcta cctagaatta caattggata
     7021 tccccgcctc ccaatccacc cacccaggga aaaagaaggg ctcgccgaaa atcaaagtta
     7081 tctccaggct cgcgcatccc accgagcggt tgacttctct ccaccacttt tcattttaac
     7141 cctcgatcgt cagacacaga aatgacaagt ttgtacaaaa aagcagagta aaggagaaga
     7201 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     7261 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     7321 tatttgcact actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac
     7381 taaccctgat tatttaaatt ttcagccaac acttgtcact actttctgtt atggtgttca
     7441 atgcttctcg agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc
     7501 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
     7561 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt
     7621 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
     7681 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
     7741 catggcagac aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac
     7801 taactaatct gatttaaatt ttcagaactt caaaattaga cacaacattg aagatggaag
     7861 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     7921 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     7981 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     8041 actatacaaa tagcattcgt agaattccaa ctgagcgccg gtcgctacca ttaccaactt
     8101 gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac
     8161 taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt
     8221 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt
     8281 gttattatca aaaaaacttc ttcttaattt ctttgttttt tagcttcttt taagtcacct
     8341 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     8401 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     8461 gtacacttct tatgtttttt ttacttctga taaatttttt ttgaaacatc atagaaaaaa
     8521 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc
     8581 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt
     8641 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aatgtccttt cttttgcttt
     8701 ttgggggttt cccctattgt ttgtcaagag tttcgaggac ggcgtttttc ttgctaaaat
     8761 cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca
     8821 gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc
     8881 ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt
     8941 aacccagctt tcttgtacaa agttgatatc tcgacccggg aattccggac cggtaccagc
     9001 tttgggcccg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcgctg
     9061 caacattttg ctgccggtca tgactgggaa aaccctggcg ttacccaact taatcgcctt
     9121 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     9181 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     9241 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9301 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9361 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9421 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9481 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9541 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9601 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     9661 cttacaattt ag
//
