LOCUS Exported 794 bp ds-DNA linear SYN 27-MAY-2021 DEFINITION S2TAG/E17TAG GFP reporter. ACCESSION . VERSION . KEYWORDS GFP S2TAG/E17TAG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 794) AUTHORS Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J TITLE A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. JOURNAL Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. PUBMED 26350500 REFERENCE 2 (bases 1 to 794) AUTHORS . TITLE Direct Submission JOURNAL Exported May 27, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..794 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(40..57) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS complement(64..777) /codon_start=1 /product="GFP variant optimized for excitation by UV light" /label=GFPuv /translation="M*KGEELFTGVVPILV*LDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" primer_bind complement(104..124) /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" ORIGIN 1 tagcagccgg atcaagcttt gaattgagct cgcccttcag tggtggtggt ggtggtgctc 61 gagtttgtag agctcatcca tgccatgtgt aatcccagca gcagttacaa actcaagaag 121 gaccatgtgg tcacgctttt cgttgggatc tttcgaaagg gcagattgtg tcgacaggta 181 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc 241 tgctagttga acggatccat cttcaatgtt gtggcgaatt ttgaagttag ctttgattcc 301 attcttttgt ttgtctgccg tgatgtatac attgtgtgag ttatagttgt attcgagttt 361 gtgtccgaga atgtttccat cttctttaaa atcaatacct tttaactcga tacgattaac 421 aagggtatca ccttcaaact tgacttcagc acgcgtcttg tagttcccgt catctttgaa 481 agatatagtg cgttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg 541 tttcatgtga tccggataac gggaaaagca ttgaacacca taagagaaag tagtgacaag 601 tgttggccat ggaacaggta gttttccagt agtgcaaata aatttaaggg taagttttcc 661 gtatgttgca tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc 721 atctaactaa acaagaattg ggacaactcc agtgaaaagt tcttctcctt tctacatggt 781 acctttctcc tctt //