LOCUS       Exported               20611 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  TALENs targeting ANT1 locus, donor molecule with 5' homology 
            arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon 
            based onTomato Yellow Leaf Curl Virus.
ACCESSION   .
VERSION     .
KEYWORDS    pTC208
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20611)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 20611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..20611
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        907..1252
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1286..1351
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1358..1378
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4712..4768
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4772..4837
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4844..4864
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7595..8182
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     promoter        9593..9772
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        9625..9808
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9866..10663
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9923..9942)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10533..10552
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10689..10863
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        11074..11419
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    14334..14358
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             15658..16287
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             16721..17788
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      17854..18048
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(18284..18306)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    18392..18532
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18447..18466
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18547..18564)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18718..19306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18798..18817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(19393..20187)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtagagct caaagtttaa cgcgtcgatt aagttgactt ggtcaatcgg
      121 tgtctctcaa ctctctctat gtatcggtgt attggagtcc tatatatatg gagactctaa
      181 tggcataaat gtaaatatgg aactttaaat caaaaccctt acgctccaaa aagcggccat
      241 ccgtataata ttaccggatg gccgcgattt tttttttgga ccccacaacg caataactga
      301 caaagacatg cggaccaatc acctgacgcg ctcaaagctt aattgttttg tgttttctat
      361 ttaaacttgg gcaccaagta ttgtagttcg tactacaagt ttgtaccgat cggcgcgcca
      421 gatttgcctt ttcaatttca gaaagaatgc taacccacag atggttagag aggcttacgc
      481 agcaggtatc atcaagacga tctacccgag caataatctc caggaaatca aataccttcc
      541 caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa
      601 agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca
      661 caaaccaagg caagtaatag agattggagt ctctaaaaag gtagttccca ctgaatcaaa
      721 ggccatggag tcaaagattc aaatagagga cctaacagaa ctcgccgtaa agactggcga
      781 acagttcata cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt
      841 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag
      901 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
      961 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
     1021 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
     1081 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     1141 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     1201 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagaa cacgggggac
     1261 tcctgcagga aaaatggctt cctccgatta taaggatcac gatggcgact acaaggacca
     1321 cgatattgac tacaaagacg atgacgataa aatggctcct aagaaaaagc gcaaagtcgg
     1381 tatccatggc gttccctcta gaatggtgga tctacgcacg ctcggctaca gtcagcagca
     1441 gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg aggcactggt
     1501 gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg cagcgttagg
     1561 gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga cacacgaaga
     1621 catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct tgctcacgga
     1681 tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg tgaagattgc
     1741 aaaacgtggc ggcgtgaccg caatggaggc agtgcatgcc agcaggaatg cgttaacagg
     1801 tgcccccctg aacctgaccc cggaccaagt ggtggctatc gccagcaaca ttggcggcaa
     1861 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
     1921 cccggaccaa gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt
     1981 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
     2041 tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
     2101 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg
     2161 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
     2221 cctgaccccg gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga
     2281 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgactc cggaccaagt
     2341 ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
     2401 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
     2461 cattggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
     2521 ccatggcctg accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc
     2581 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
     2641 ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg
     2701 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
     2761 cagcaacggt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
     2821 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa
     2881 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
     2941 cccggaccaa gtggtggcta tcgccagcaa cattggcggc aagcaagcgc tcgaaacggt
     3001 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
     3061 tatcgccagc aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
     3121 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg
     3181 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
     3241 cctgaccccg gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga
     3301 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgactc cggaccaagt
     3361 ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
     3421 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
     3481 cattggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
     3541 ccatggcctg actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc
     3601 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgactccgga
     3661 ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa cggtgcagcg
     3721 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
     3781 cagcaacggt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
     3841 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa
     3901 gcaagcgctc gaaagcattg tggcccagct gagccggcct gatccggcgt tggccgcgtt
     3961 gaccaacgac cacctcgtcg ccttggcctg cctcggcgga cgtcctgcca tggatgcagt
     4021 gaaaaaggga ttgccgcacg cgccggaatt gatcagaaga gtcaatcgcc gtattggcga
     4081 acgcacgtcc catcgcgttg ccggatccca gctggtgaag tccgagctgg aagaaaaaaa
     4141 gagcgagctg cgccacaagc tcaagtacgt gccccacgag tacatcgagc tgatcgagat
     4201 cgcccgcaac agcacccaag accgcatcct ggagatgaaa gtgatggagt tcttcatgaa
     4261 ggtgtacggc taccgcggca agcacctggg cggctcccgc aagcccgatg gcgccatcta
     4321 caccgtgggc tcccccatcg actatggcgt cattgtcgac accaaggcct actccggcgg
     4381 ctacaactta cccatcggtc aggccgacga gatgcaacgc tacgtgaagg agaaccagac
     4441 ccgcaataag cacattaatc ccaacgagtg gtggaaggtg tacccctcct ccgtgaccga
     4501 gttcaaattc ctgttcgtgt ccggccactt caagggcaat tataaggccc aactgacccg
     4561 cctgaaccgc aagaccaact gcaacggcgc cgtgctgtcc gtggaggaac tgctgatcgg
     4621 cggcgagatg atcaaggctg gtaccctgac cctggaagag gtgcgccgca agttcaacaa
     4681 tggtgaaatc aatttcaggt ccggaagcgg cgccacgaac ttctctctgt taaagcaagc
     4741 aggggacgtg gaagaaaacc cagggcccat ggactacaaa gaccatgacg gtgattataa
     4801 agatcatgac atcgattaca aggatgacga tgacaagatg gcccccaaga agaagaggaa
     4861 ggtgggcatt cacggggtgc cggctagcat ggtggatcta cgcacgctcg gctacagtca
     4921 gcagcagcaa gagaagatca aaccgaaggt gcgttcgaca gtggcgcagc accacgaggc
     4981 actggtgggc catgggttta cacacgcgca catcgttgcg ctcagccaac acccggcagc
     5041 gttagggacc gtcgctgtca cgtatcagca cataatcacg gcgttgccag aggcgacaca
     5101 cgaagacatc gttggcgtcg gcaaacagtg gtccggcgca cgcgccctgg aggccttgct
     5161 cacggatgcg ggggagttga gaggtccgcc gttacagttg gacacaggcc aacttgtgaa
     5221 gattgcaaaa cgtggcggcg tgaccgcaat ggaggcagtg catgcatcgc gcaatgcact
     5281 gacgggtgcc cccctgaacc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg
     5341 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
     5401 cctgaccccg gaccaagtgg tggctatcgc cagcaacggt ggcggcaagc aagcgctcga
     5461 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt
     5521 ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
     5581 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
     5641 cggtggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
     5701 ccatggcctg accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc
     5761 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
     5821 ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg
     5881 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
     5941 cagcaacggt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
     6001 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca atggcggcaa
     6061 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
     6121 cccggaccaa gtggtggcta tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt
     6181 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg actccggacc aagtggtggc
     6241 tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
     6301 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg
     6361 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
     6421 cctgaccccg gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga
     6481 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt
     6541 ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
     6601 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
     6661 cattggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
     6721 ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg gcaagcaagc
     6781 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
     6841 ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg
     6901 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
     6961 cagcaacggt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
     7021 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca ttggcggcaa
     7081 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
     7141 tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt
     7201 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
     7261 tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
     7321 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg
     7381 cggcaagcaa gcgctcgaaa gcattgtggc ccagctgagc cggcctgatc cggcgttggc
     7441 cgcgttgacc aacgatcatc tggtggcact cgcgtgcctc ggcggacgtc ctgccatgga
     7501 tgcagtgaaa aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat
     7561 tggcgaacgc acgtcccatc gcgttgccag atctcaacta gtcaaaagtg aactggagga
     7621 gaagaaatct gaacttcgtc ataaattgaa atatgtgcct catgaatata ttgaattaat
     7681 tgaaattgcc agaaattcca ctcaggatag aattcttgaa atgaaggtaa tggaattttt
     7741 tatgaaagtt tatggatata gaggtaaaca tttgggtgga tcaaggaaac cggacggagc
     7801 aatttatact gtcggatctc ctattgatta cggtgtgatc gtggatacta aagcttatag
     7861 cggaggttat aatctgccaa ttggccaagc agatgaaatg gagcgatatg tcgaagaaaa
     7921 tcaaacacga gacaaacatc tcaaccctaa tgaatggtgg aaagtctatc catcttctgt
     7981 aacggaattt aagtttttat ttgtgagtgg tcactttaaa ggaaactaca aagctcagct
     8041 tacacgatta aatcatatca ctaattgtaa tggagctgtt cttagtgtag aagagctttt
     8101 aattggtgga gaaatgatta aagccggcac attaacctta gaggaagtga ggcggaaatt
     8161 taataacggc gagataaact tttaatagct cgagatatga agatgaagat gaaatatttg
     8221 gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt
     8281 atgaatttgt ggctttttct aatattaaat gaatgtaaga tcacattata atgaataaac
     8341 aaatgtttct ataatccatt gtgaatgttt tgttggatct cttctgcagc atataactac
     8401 tgtatgtgct atggtatgga ctatggaata tgattaaaga taaggagctc cggcgagctt
     8461 tgggtacgtc acgtggctcg agcgcgtagt cctcggtaac cacaacactt gtcggtgaga
     8521 ttatttaatg ctgattagat tagacaaaaa ttaattagtt ttgagtagtg gcgtaagtgt
     8581 aaataattag tctctttttt aacttagaaa atagtttaat ccttagtata aatagtcaaa
     8641 atcactggaa tgaaaaacag tttttaattt ttccaaattt gattctgata ccatgttaaa
     8701 ttcgtggttc aaaatcactg caatgaaaag agcaatattg tttaactttt tttaggaaaa
     8761 tcgaattgat ttatagtcag ttgatataga gtgaatacat aaggaacata tacagttgat
     8821 acaattgtat aattcgttca tacacttaat acaaagtgaa cccacaagga acatatacac
     8881 ttaatataat tgtattcctt gatacaaacc aattttgttc gtgtctctac tctctatttc
     8941 aatttcgctt gactctttac tttttctaat atgtagctat aaatcgtaat taaacaatac
     9001 tatatctcta aatctcttat taagctcaaa ctatggtcat attcgaaaaa atccttttaa
     9061 atattggtcc cctctcacga ttaatgatag ttataactaa cattcaaatt ttagttgtac
     9121 ttgacatcta aaacttaaaa aatagtacaa gttaactttt tctttttttt aaaaaaagga
     9181 aatacttgta tttatttttt taatatatag ttatattttt ggttatttga aaatacttga
     9241 tctgtcatgt atgctcagtt aaatatcgtc acattataga gaaaaaagta ataggagaaa
     9301 aaaattaaaa attatttcga aaaatcaaaa tttttttttg attgaaatga aagatgggtt
     9361 tcccaatcga ggctggcagg ataggtacat tgggaaattt ggatttgtgt gttgaaaatg
     9421 attgttcaat ttggctttta taacatttgt cgtttataat ttgtagaagg ctctctacaa
     9481 gttggactga aggcgggaaa cgacaatctg atcatgagcg gagaattaag ggagtcacgt
     9541 tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca
     9601 acgttgaagg agccactcag ccgcgggttt ctggagttta atgagctaag cacatacgtc
     9661 agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag caaatatttc
     9721 ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat tagagtctca
     9781 tattcactct caatccaaat aatctgcacc ggatccgcta gaggatctcg acctgcaaga
     9841 tcccgggggg caatgagatc ctaggatggg gattgaacaa gatggattgc acgcaggttc
     9901 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     9961 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
    10021 cgacctgtcc ggtgccctga atgaactcca ggacgaggca gcgcggctat cgtggctggc
    10081 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
    10141 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
    10201 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
    10261 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
    10321 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
    10381 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgacac atggcgatgc
    10441 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
    10501 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
    10561 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
    10621 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgattaatta acgatcgaca
    10681 agctctattt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
    10741 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
    10801 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
    10861 atcccccgaa ttaattcggc gttaattcag catggagtca aagattcaaa tagaggacct
    10921 aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga
    10981 caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat
    11041 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
    11101 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
    11161 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
    11221 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
    11281 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
    11341 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
    11401 atttcatttg gagagaacac gggggactct tgacagtagt ataatatatt atcaaattat
    11461 tatgaacagt acatctatgt cttcattggg agtgagaaaa ggttcatgga ctgatgaaga
    11521 agattttctt ctaagaaaat gtattgataa gtatggtgaa ggaaaatggc atcttgttcc
    11581 cataagagct ggtaactatt aaattaacta tcacgttatt tttatttgtc tttctgtctc
    11641 attttatttg acgttattac gaatatcatc tgaaaatgta cgtgcaggtc tgaatagatg
    11701 tcggaaaagt tgtagattga ggtggctgaa ttatctaagg ccacatatca agagaggtga
    11761 ctttgaacaa gatgaagtgg atctcatttt gaggcttcat aagctcttag gcaacaggca
    11821 tgcaagttta tgttttgaca aaatttgatt agtatatatt atatatacgt gtgactattt
    11881 catctaaatg ttacgttatt ttacgtagat ggtcacttat tgctggtaga cttcccggaa
    11941 ggacagctaa cgatgtgaaa aactattgga acactaatct tctaaggaag ttaaatacta
    12001 ctaaaattgt tcctcgcgaa aagattaaca ataagtgtgg agaaattagt actaagattg
    12061 aaattataaa acctcaacga cgcaagtatt tctcaagcac aatgaagaat gttacaaaca
    12121 ataatgtaat tttggacgag gaggaacatt gcatttaaat gagtgtactt caagtcacac
    12181 cggcgagtgt ttgatcgccg gcggtacaaa gtggtagtaa catagatgac accgcgcgcg
    12241 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt
    12301 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta
    12361 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag
    12421 gattcaatct taagaaactt tattgccaaa tgtttgaacg atcggggaaa ttcctaataa
    12481 atattaaatt ttattatatg cgaactgtct acaaatgttg tttgcgtcaa tgcatcccat
    12541 agtacataat tcactgctct aattacatta ttcaaactaa ttacacccaa attattaaga
    12601 aatttcaaca cttgtgtttt aaataccctt aagaaacgac cagtcggagg ctgtgaggtc
    12661 atccagattc ggtaggttag gaaacatttg tgtatcccca acactttcct caggttgtga
    12721 ttgaactgta tccggacggt gatgatgtct tcgttcatca cgaagggccg gttttggtgc
    12781 tctgttatct tgaaatatag gggattttga atctcccaga taaacacgcc attctctgct
    12841 tgagctgcag tgatgagttc ccctgtgcgt gaatccatgg tcgtggcagg ttattgctat
    12901 gaagtaagaa cagccacacg gtagatcaac tcgtcgacgt ctggtcccct tcttggctag
    12961 cctgtgctgt actttgattg gtacctgagt agagtgggcc ttcgagggtg acgaaggtcg
    13021 cattttttaa agcccagttt tttagtgcag aattcttttc ctcatccaag tattctttat
    13081 agctggagtt gggtcctgga ttgcagagga aggtagtggg aattccgcct ttaatttgaa
    13141 ctggcttccc gtactttgta tttgattgcc agtccctttg ggcccccatg aattctttaa
    13201 agtgctttag gtagtggggg tctacgtcat caatgacgtt gtaccaggca tcattactgt
    13261 aaacctttgg gctaaggtct agatgaccac acaaataatt atgtggtccc aatgatctgg
    13321 cccacatagt cttccccgtc ctactgtcac cctcaattac tatacttatg ggtctcagag
    13381 gccgcgcagc gggactgcag acgttctcgg cagcccactc ttcaagttct tctggaactt
    13441 ggtcgaaaga agaagataaa aaaggtgaaa cataaacatc cattggaggt gtaaaaatcc
    13501 tatctaaatt agaatttaaa ttgtgaaatt gtaaaacaaa atctttggga gctttctccc
    13561 tcaatatatt aagggcctga gctttggacc ctgaattgat tgcctcggca tatgcgtcgt
    13621 tggcagattg gcaacctcct ctagctgatc gtccatcgac ttggaaaact ccatgatcaa
    13681 tgacgtctcc gtctttttcc atgtaggatt tgacatcgct tgagctctta gctccctgaa
    13741 tgttcggatg gaaatgtgct gacctggttg gggatgtgag gtcgaagaat ctattgtttt
    13801 tgcactggaa ctttccttcg aactggatga gcacatgcaa gtgaggagtc ccatcttcgt
    13861 gaagctctct gcaaattcta ataaattttt tgaaagtggg tgtttggaga tttaataatt
    13921 tgggaaagtg ctcttcttta gttagagagc acttgggata agtgagaaaa taatttttgg
    13981 catttatttt aaagcgattg ggggctgcca tgttgacttg gtcaatcggt gtctctcaac
    14041 tctctctatg tatcggtgta ttggagtcct atatatatgg agactctaat ggcataaatg
    14101 taaatatgga actttaaatc aaaaccctta cgctccaaaa agcggccatc cgtataatat
    14161 taccggatgg ccgcgatttt ttttttggac cccacaacgc aataactgac aaagacatgc
    14221 ggaccaatca cctgacgcgc tcaaagctta attgttttgt gttttctatt taaacttggg
    14281 caccaagtat tgtagttcgt acttggggcc ctcgagttta aactatcagt gtttgacagg
    14341 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataacgg atatttaaaa
    14401 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
    14461 ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
    14521 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
    14581 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
    14641 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
    14701 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
    14761 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
    14821 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
    14881 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
    14941 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
    15001 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
    15061 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
    15121 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
    15181 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
    15241 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
    15301 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
    15361 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
    15421 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga
    15481 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
    15541 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
    15601 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
    15661 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
    15721 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
    15781 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
    15841 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
    15901 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
    15961 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
    16021 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
    16081 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
    16141 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
    16201 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
    16261 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
    16321 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
    16381 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
    16441 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
    16501 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
    16561 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
    16621 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc
    16681 aatggcactg gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc
    16741 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
    16801 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
    16861 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
    16921 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
    16981 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
    17041 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
    17101 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
    17161 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
    17221 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
    17281 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
    17341 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
    17401 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
    17461 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
    17521 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
    17581 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
    17641 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
    17701 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
    17761 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
    17821 agcaggggaa aaaggtcgaa aaggcctctt tcctgtggat agcacgtaca ttgggaaccc
    17881 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
    17941 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
    18001 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
    18061 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
    18121 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
    18181 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgacagcc ggcgcccaca
    18241 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    18301 tcccggaaac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    18361 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
    18421 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
    18481 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
    18541 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    18601 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    18661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    18721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    18781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    18841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    18901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    18961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    19021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    19081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    19141 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    19201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    19261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    19321 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    19381 gcattctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
    19441 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
    19501 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
    19561 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
    19621 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
    19681 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
    19741 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
    19801 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
    19861 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
    19921 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
    19981 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
    20041 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
    20101 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
    20161 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
    20221 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
    20281 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
    20341 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
    20401 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
    20461 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
    20521 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
    20581 gctgccggtc ggggagctgt tggctggctg g
//
