LOCUS Exported 17997 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b. ACCESSION . VERSION . KEYWORDS pTC217 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 17997) AUTHORS Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF TITLE High-frequency, precise modification of the tomato genome. JOURNAL Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9. PUBMED 26541286 REFERENCE 2 (bases 1 to 17997) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..17997 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..25 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" promoter 966..1311 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 1345..5448 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5461..5481 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_RNA 6229..6304 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" promoter 7456..7635 /label=NOS promoter /note="nopaline synthase promoter" promoter 7488..7671 /function="NOS promoter" /label=NOS promoter /note="nopaline synthase promoter" CDS 7729..8526 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7786..7805) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8396..8415 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 8552..8726 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" promoter 8937..9282 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" misc_feature 11720..11744 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 13044..13673 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 14107..15174 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" rep_origin 15240..15434 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(15670..15692) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 15778..15918 /label=bom /note="basis of mobility region from pBR322" primer_bind 15833..15852 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(15933..15950) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(16104..16692) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(16184..16203) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(16779..17573) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" ORIGIN 1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 61 gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact 121 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat 181 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt 241 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga 301 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa 361 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat 421 caggtatttc cgttggatac gaattattcg tacgaccctc ggtaccgatc ggcgcgccag 481 atttgccttt tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca 541 gcaggtatca tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc 601 aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa 661 gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac 721 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag 781 gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa 841 cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg 901 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg 961 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca 1021 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat 1081 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat 1141 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag 1201 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct 1261 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact 1321 cctgcaggta gatcgctcgt cgacatggat aagaagtact ctatcggact cgatatcgga 1381 actaactctg tgggatgggc tgtgatcacc gatgagtaca aggtgccatc taagaagttc 1441 aaggttctcg gaaacaccga taggcactct atcaagaaaa accttatcgg tgctctcctc 1501 ttcgattctg gtgaaactgc tgaggctacc agactcaaga gaaccgctag aagaaggtac 1561 accagaagaa agaacaggat ctgctacctc caagagatct tctctaacga gatggctaaa 1621 gtggatgatt cattcttcca caggctcgaa gagtcattcc tcgtggaaga agataagaag 1681 cacgagaggc accctatctt cggaaacatc gttgatgagg tggcatacca cgagaagtac 1741 cctactatct accacctcag aaagaagctc gttgattcta ctgataaggc tgatctcagg 1801 ctcatctacc tcgctctcgc tcacatgatc aagttcagag gacacttcct catcgagggt 1861 gatctcaacc ctgataactc tgatgtggat aagttgttca tccagctcgt gcagacctac 1921 aaccagcttt tcgaagagaa ccctatcaac gcttcaggtg tggatgctaa ggctatcctc 1981 tctgctaggc tctctaagtc aagaaggctt gagaacctca ttgctcagct ccctggtgag 2041 aagaagaacg gacttttcgg aaacttgatc gctctctctc tcggactcac ccctaacttc 2101 aagtctaact tcgatctcgc tgaggatgca aagctccagc tctcaaagga tacctacgat 2161 gatgatctcg ataacctcct cgctcagatc ggagatcagt acgctgattt gttcctcgct 2221 gctaagaacc tctctgatgc tatcctcctc agtgatatcc tcagagtgaa caccgagatc 2281 accaaggctc cactctcagc ttctatgatc aagagatacg atgagcacca ccaggatctc 2341 acacttctca aggctcttgt tagacagcag ctcccagaga agtacaaaga gattttcttc 2401 gatcagtcta agaacggata cgctggttac atcgatggtg gtgcatctca agaagagttc 2461 tacaagttca tcaagcctat cctcgagaag atggatggaa ccgaggaact cctcgtgaag 2521 ctcaatagag aggatcttct cagaaagcag aggaccttcg ataacggatc tatccctcat 2581 cagatccacc tcggagagtt gcacgctatc cttagaaggc aagaggattt ctacccattc 2641 ctcaaggata acagggaaaa gattgagaag attctcacct tcagaatccc ttactacgtg 2701 ggacctctcg ctagaggaaa ctcaagattc gcttggatga ccagaaagtc tgaggaaacc 2761 atcacccctt ggaacttcga agaggtggtg gataagggtg ctagtgctca gtctttcatc 2821 gagaggatga ccaacttcga taagaacctt ccaaacgaga aggtgctccc taagcactct 2881 ttgctctacg agtacttcac cgtgtacaac gagttgacca aggttaagta cgtgaccgag 2941 ggaatgagga agcctgcttt tttgtcaggt gagcaaaaga aggctatcgt tgatctcttg 3001 ttcaagacca acagaaaggt gaccgtgaag cagctcaaag aggattactt caagaaaatc 3061 gagtgcttcg attcagttga gatttctggt gttgaggata ggttcaacgc atctctcgga 3121 acctaccacg atctcctcaa gatcattaag gataaggatt tcttggataa cgaggaaaac 3181 gaggatatct tggaggatat cgttcttacc ctcaccctct ttgaagatag agagatgatt 3241 gaagaaaggc tcaagaccta cgctcatctc ttcgatgata aggtgatgaa gcagttgaag 3301 agaagaagat acactggttg gggaaggctc tcaagaaagc tcattaacgg aatcagggat 3361 aagcagtctg gaaagacaat ccttgatttc ctcaagtctg atggattcgc taacagaaac 3421 ttcatgcagc tcatccacga tgattctctc acctttaaag aggatatcca gaaggctcag 3481 gtttcaggac agggtgatag tctccatgag catatcgcta acctcgctgg atctcctgca 3541 atcaagaagg gaatcctcca gactgtgaag gttgtggatg agttggtgaa ggtgatggga 3601 aggcataagc ctgagaacat cgtgatcgaa atggctagag agaaccagac cactcagaag 3661 ggacagaaga actctaggga aaggatgaag aggatcgagg aaggtatcaa agagcttgga 3721 tctcagatcc tcaaagagca ccctgttgag aacactcagc tccagaatga gaagctctac 3781 ctctactacc tccagaacgg aagggatatg tatgtggatc aagagttgga tatcaacagg 3841 ctctctgatt acgatgttga tcatatcgtg ccacagtcat tcttgaagga tgattctatc 3901 gataacaagg tgctcaccag gtctgataag aacaggggta agagtgataa cgtgccaagt 3961 gaagaggttg tgaagaaaat gaagaactat tggaggcagc tcctcaacgc taagctcatc 4021 actcagagaa agttcgataa cttgactaag gctgagaggg gaggactctc tgaattggat 4081 aaggcaggat tcatcaagag gcagcttgtg gaaaccaggc agatcactaa gcacgttgca 4141 cagatcctcg attctaggat gaacaccaag tacgatgaga acgataagtt gatcagggaa 4201 gtgaaggtta tcaccctcaa gtcaaagctc gtgtctgatt tcagaaagga tttccaattc 4261 tacaaggtga gggaaatcaa caactaccac cacgctcacg atgcttacct taacgctgtt 4321 gttggaaccg ctctcatcaa gaagtatcct aagctcgagt cagagttcgt gtacggtgat 4381 tacaaggtgt acgatgtgag gaagatgatc gctaagtctg agcaagagat cggaaaggct 4441 accgctaagt atttcttcta ctctaacatc atgaatttct tcaagaccga gattaccctc 4501 gctaacggtg agatcagaaa gaggccactc atcgagacaa acggtgaaac aggtgagatc 4561 gtgtgggata agggaaggga tttcgctacc gttagaaagg tgctctctat gccacaggtg 4621 aacatcgtta agaaaaccga ggtgcagacc ggtggattct ctaaagagtc tatcctccct 4681 aagaggaact ctgataagct cattgctagg aagaaggatt gggaccctaa gaaatacggt 4741 ggtttcgatt ctcctaccgt ggcttactct gttctcgttg tggctaaggt tgagaaggga 4801 aagagtaaga agctcaagtc tgttaaggaa cttctcggaa tcactatcat ggaaaggtca 4861 tctttcgaga agaacccaat cgatttcctc gaggctaagg gatacaaaga ggttaagaag 4921 gatctcatca tcaagctccc aaagtactca ctcttcgaac tcgagaacgg tagaaagagg 4981 atgctcgctt ctgctggtga gcttcaaaag ggaaacgagc ttgctctccc atctaagtac 5041 gttaactttc tttacctcgc ttctcactac gagaagttga agggatctcc agaagataac 5101 gagcagaagc aacttttcgt tgagcagcac aagcactact tggatgagat catcgagcag 5161 atctctgagt tctctaaaag ggtgatcctc gctgatgcaa acctcgataa ggtgttgtct 5221 gcttacaaca agcacagaga taagcctatc agggaacagg cagagaacat catccatctc 5281 ttcaccctta ccaacctcgg tgctcctgct gctttcaagt acttcgatac aaccatcgat 5341 aggaagagat acacctctac caaagaagtg ctcgatgcta ccctcatcca tcagtctatc 5401 actggactct acgagactag gatcgatctc tcacagctcg gtggtgattc aagggctgat 5461 cctaagaaga agaggaaggt ttgactcgag atatgaagat gaagatgaaa tatttggtgt 5521 gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga 5581 atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat 5641 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta 5701 tgtgctatgg tatggactat ggaatatgat taaagataag gagctccggt gacggaccca 5761 tggcttcgtt gaacaacgga aactcgactt gccttccgca caatacatca tttcttctta 5821 gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt acattttctt 5881 gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg tatcttgttt 5941 catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt tgattgaata 6001 aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg aatctgaaag 6061 aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat aggcccattt 6121 aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga agctgagttt 6181 atatacagct agagtcgaag tagtgattga caatttaata caccttttgt tttagagcta 6241 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg 6301 gtgctttttt tcccggcgag ctttgggtac gtcacgtggc tcgagcgcgt agtcctcggt 6361 aaccacaaca cttgtcggtg agattattta atgctgatta gattagacaa aaattaatta 6421 gttttgagta gtggcgtaag tgtaaataat tagtctcttt tttaacttag aaaatagttt 6481 aatccttagt ataaatagtc aaaatcactg gaatgaaaaa cagtttttaa tttttccaaa 6541 tttgattctg ataccatgtt aaattcgtgg ttcaaaatca ctgcaatgaa aagagcaata 6601 ttgtttaact ttttttagga aaatcgaatt gatttatagt cagttgatat agagtgaata 6661 cataaggaac atatacagtt gatacaattg tataattcgt tcatacactt aatacaaagt 6721 gaacccacaa ggaacatata cacttaatat aattgtattc cttgatacaa accaattttg 6781 ttcgtgtctc tactctctat ttcaatttcg cttgactctt tactttttct aatatgtagc 6841 tataaatcgt aattaaacaa tactatatct ctaaatctct tattaagctc aaactatggt 6901 catattcgaa aaaatccttt taaatattgg tcccctctca cgattaatga tagttataac 6961 taacattcaa attttagttg tacttgacat ctaaaactta aaaaatagta caagttaact 7021 ttttcttttt tttaaaaaaa ggaaatactt gtatttattt ttttaatata tagttatatt 7081 tttggttatt tgaaaatact tgatctgtca tgtatgctca gttaaatatc gtcacattat 7141 agagaaaaaa gtaataggag aaaaaaatta aaaattattt cgaaaaatca aaattttttt 7201 ttgattgaaa tgaaagatgg gtttcccaat cgaggctggc aggataggta cattgggaaa 7261 tttggatttg tgtgttgaaa atgattgttc aatttggctt ttataacatt tgtcgtttat 7321 aatttgtaga aggctctcta caagttggac tgaaggcggg aaacgacaat ctgatcatga 7381 gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta 7441 cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt 7501 ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt 7561 cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc 7621 ctcggtatcc aattagagtc tcatattcac tctcaatcca aataatctgc accggatccg 7681 ctagaggatc tcgacctgca agatcccggg gggcaatgag atcctaggat ggggattgaa 7741 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 7801 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 7861 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact ccaggacgag 7921 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 7981 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 8041 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 8101 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 8161 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 8221 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 8281 ctcgtcgtga cacatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 8341 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 8401 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 8461 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 8521 ttctgattaa ttaacgatcg acaagctcta ttttctccat aataatgtgt gagtagttcc 8581 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc 8641 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac 8701 caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagcatggag 8761 tcaaagattc aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata 8821 cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac 8881 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag 8941 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt 9001 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat 9061 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca 9121 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 9181 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac 9241 ccttcctcta tataaggaag ttcatttcat ttggagagaa cacgggggac tcttgacagt 9301 agtataatat attatcaaat tattatgaac agtacatcta tgtcttcatt gggagtgaga 9361 aaaggttcat ggactgatga agaagatttt cttctaagaa aatgtattga taagtatggt 9421 gaaggaaaat ggcatcttgt tcccataaga gctggtaact attaaattaa ctatcacgtt 9481 atttttattt gtctttctgt ctcattttat ttgacgttat tacgaatatc atctgaaaat 9541 gtacgtgcag gtctgaatag atgtcggaaa agttgtagat tgaggtggct gaattatcta 9601 aggccacata tcaagagagg tgactttgaa caagatgaag tggatctcat tttgaggctt 9661 cataagctct taggcaacag gcatgcaagt ttatgttttg acaaaatttg attagtatat 9721 attatatata cgtgtgacta tttcatctaa atgttacgtt attttacgta gatggtcact 9781 tattgctggt agacttcccg gaaggacagc taacgatgtg aaaaactatt ggaacactaa 9841 tcttctaagg aagttaaata ctactaaaat tgttcctcgc gaaaagatta acaataagtg 9901 tggagaaatt agtactaaga ttgaaattat aaaacctcaa cgacgcaagt atttctcaag 9961 cacaatgaag aatgttacaa acaataatgt aattttggac gaggaggaac attgcattta 10021 aatgagtgta cttcaagtca caccggcgag tgtttgatcg ccggcggtac cgagtgtact 10081 tcaagtcagt gggaaatcaa taaaatgatt attttatgaa tatatttcat tgtgcaagta 10141 gatagaaatt acatatgtta cataacacac gaaataaaca aaaaaagaca atccaaaaac 10201 aaacacccca aaaaaaataa tcactttaga taaactcgta tgaggagagg cacgttcagt 10261 gactcgacga ttcccgagca aaaaaagtct ccccgtcaca catgtagtgg gtgacgcaat 10321 tatctttaaa gtaatccttc tgttgacttg tcattgataa catccagtct tcgtcaggat 10381 tgcaaagaat tatagaaggg atcccacctt ttattttctt cttttttcca tatttagggt 10441 tgacagtgaa atcagactgg caacctatta attgcttcca caatgggacg aacttgaagg 10501 ggatgtcgtc gatgatatta taggtggcgt gttcatcgta gttggtgaaa tcgatggtac 10561 cgttccaata gttgtgtcgt ccgagacttc tagcccaggt ggtctttccg gtacgagttg 10621 gtccgcagat gtagaggctg gggtgtcgga ttccattcct tccattgtcc ttgttaaatc 10681 ggccatccat tcaaggtcag attgagcttg ttggtatgag acaggatgta tgtaagtata 10741 agcgtctatg cttacatggt atagatgggt ttccctccag gagtgtagat cttcgtggca 10801 gcgaagatct gattctgtga agggcgacac atacggttca ggttgtggag ggaataattt 10861 gttggctgaa tattccagcc attgaagctt tgttgcccat tcatgaggga attcttcctt 10921 gatcatgtca agatattcct ccttagacgt tgcagtctgg ataatagttc tccatcgtgc 10981 gtcagatttg cgaggagaaa ccttatgatc tcggaaatct cctctggttt taatatctcc 11041 gtcctttgat atgtaatcaa ggacttgttt agagtttcta gctggctgga tattagggtg 11101 atttccttca aaatcgaaaa aagaaggatc cctaatacaa ggttttttat caagctggag 11161 aagagcatga tagtgggtag tgccatcttg atgaagctca gaagcaacac caaggaagaa 11221 aataagaaaa ggtgtgagtt tctcccagag aaactggaat aaatcatctc tttgagatga 11281 gcacttggga taggtaagga aaacatattt agattggagt ctgaagttct tactagcaga 11341 aggcatgttg ttgtgactcc gaggggttgc ctcaaactct atcttataac cggcgtggag 11401 gcatggaggc aggggtattt tggtcatttt aatagatagt ggaaaatgac gtggaattta 11461 cttaaagacg aagtctttgc gacaaggggg ggcccacgcc gaatttaata ttaccggcgt 11521 ggccccccct tatcgcgagt gctttagcac gagcggtcca gatttaaagt agaaaatttc 11581 ccgcccacta gggttaaagg tgttcacact ataaaagcat atacgatgtg atggtatttg 11641 atggagcgta tattgtatca ggtatttccg ttggatacga attattcgta cgaccctcat 11701 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg 11761 tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt 11821 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg 11881 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc 11941 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt 12001 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat 12061 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga 12121 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa 12181 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc 12241 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact 12301 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg 12361 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga 12421 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg 12481 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga 12541 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg 12601 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg 12661 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa 12721 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc 12781 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg 12841 cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg 12901 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt 12961 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat 13021 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt 13081 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg 13141 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag 13201 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca 13261 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt 13321 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat 13381 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc 13441 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg 13501 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga 13561 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg 13621 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg 13681 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg 13741 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa 13801 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa 13861 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg 13921 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac 13981 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa 14041 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg 14101 cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg 14161 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc 14221 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg 14281 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc 14341 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt 14401 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac 14461 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg 14521 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga 14581 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga 14641 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg 14701 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag 14761 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac 14821 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac 14881 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac 14941 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac 15001 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc 15061 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc 15121 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg 15181 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg cctctttcct 15241 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt 15301 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag 15361 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc 15421 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc 15481 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc 15541 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg 15601 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga 15661 cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc tgtaagcgga 15721 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 15781 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 15841 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 15901 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 15961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 16021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 16081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 16141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 16201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 16261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 16321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 16381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 16441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 16501 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 16561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 16621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 16681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 16741 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa 16801 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg 16861 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac 16921 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc 16981 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt 17041 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag 17101 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg 17161 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg 17221 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag 17281 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag 17341 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt 17401 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca 17461 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag 17521 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat 17581 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga 17641 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa 17701 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt 17761 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca 17821 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag 17881 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc 17941 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctgg //