LOCUS Exported 957 bp ds-DNA linear SYN 12-JUN-2021 DEFINITION Transient expression in mammalian cells of an ""INT"" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter. ACCESSION . VERSION . KEYWORDS pU6_(GLuc)_INT[3xMS2_SL] SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 957) AUTHORS Shechner DM, Hacisuleyman E, Younger ST, Rinn JL TITLE Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. JOURNAL Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. PUBMED 26030444 REFERENCE 2 (bases 1 to 957) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..957 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(284..303) /label=LKO.1 5' /note="Human U6 promoter, forward primer" primer_bind complement(467..484) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(467..483) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(476..498) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(692..711) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 811..833 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(871..889) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 ggtttaaaca gtcgactcta gacttaatta agaaaaaaag caccgactcg gtgccacttt 61 ttcaagttga taacggacta gccttattta aacttgctat gctgcgaata cgagggtacg 121 ctgatgctgt accagacaga ccctgatggt gtctgagacg taccctgatg gtgtacgctc 181 gtattcgcag catagctctt aaacatagtg agtcgtatct agatcggtgt ttcgtccttt 241 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata 301 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg 361 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa ttatctctct 421 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaacactg gccgtcgttt 481 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 541 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 601 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg 661 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 721 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 781 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 841 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta 901 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgc //