LOCUS Exported 1049 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter. ACCESSION . VERSION . KEYWORDS pUBC_V5_MS2-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1049) AUTHORS Shechner DM, Hacisuleyman E, Younger ST, Rinn JL TITLE Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. JOURNAL Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. PUBMED 26030444 REFERENCE 2 (bases 1 to 1049) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1049 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(27..737) /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(107..130) /label=DsRed1-C /note="DsRed1, forward primer" primer_bind complement(305..324) /label=mCherry-F /note="mCherry, forward primer" primer_bind 574..592 /label=mCherry-R /note="mCherry, reverse primer" ORIGIN 1 gctgcaataa acaagtcgac ttatcattac ttgtacagct cgtccatgcc gccggtggag 61 tggcggccct cggcgcgctc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg 121 atgtccagct tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg 181 taggtggtct tgacctcggc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg 241 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc 301 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc 361 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg 421 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag 481 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac 541 aggatgtccc aggcgaaggg cagggggccg cccttggtca ccttcagctt ggcggtctgg 601 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgcg gccgttcacg 661 gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat gttgtcctcc 721 tcgcccttgc tcaccattcc ggagcccgag gtcgagcccg agcccttgga tccataaatg 781 ccggaattgg ccgcgattgc agatggaata ggattgccgt ccttcagcag accctgcatg 841 gctttgacga tgagctcaca gtcgctgttt gtggcgaaga ttggaatggt gagttccatg 901 ttgaggtagc ttctccagcc ggccacaggc aattcctcgc cccccacggt ttgagttgcc 961 actttcggca cttctacctt aatagtatat ttccgattct gggcggatga ttgccggaca 1021 ctacaggtga ccttgtaagc ctgggacct //