LOCUS       Exported                 888 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Cas9 activity reporter (control) with BFP and GFP..
ACCESSION   .
VERSION     .
KEYWORDS    pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    130..247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        322..562
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     322..342
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     493..512
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(732..755)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
       61 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
      121 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
      181 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
      241 aaattttcgg gtttattaca gggacagcag agatccagtt tggttagtac cgggccctac
      301 gcgtccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      361 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      421 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      481 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      541 ttatatatct tgtggaaagg acgaaacacc gggtcttcga gaagacacgt ttaagagcta
      601 tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg
      661 caccgagtcg gtgctttttt tggatccaat tctaccgggt aggggaggcg cttttcccaa
      721 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
      781 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
      841 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccc
//
