LOCUS       Exported                 812 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Cas9 activity reporter (control) with BFP and GFP..
ACCESSION   .
VERSION     .
KEYWORDS    pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      58..67
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             64..765
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
ORIGIN
        1 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atccatctag atctcgagcc
       61 accatgggca gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
      121 gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
      181 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
      241 gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac
      301 ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaggac
      361 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
      421 gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc
      481 ggctgggagg ccttcaccga gaccctgtac cccgctgacg gcggcctgga aggcagaaac
      541 gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat
      601 agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga
      661 ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg
      721 gccagatact gcgacctccc tagcaaactg gggcacaagc ttaatggagg gggcagcggc
      781 ggggaaggaa ggggctccct gctcacctgc gg
//
