LOCUS       Exported                 624 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Lentiviral vector expressing Cas9 fused with the Blasticidin 
            resistant gene at the C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    pKLV2-EF1a-Cas9Bsd-W
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tccagttcgc tcaggccgcc tctctcggcc ttggtcagat tgtcgaactt tctctgggta
       61 atcagcttgg cgttcagcag ctgccgccag tagttcttca tcttcttcac gacctcttcg
      121 gagggcacgt tgtcgctctt gccccggttc ttgtcgcttc tggtcagcac cttgttgtcg
      181 atggagtcgt ccttcagaaa gctctgaggc acgatatggt ccacatcgta gtcggacagc
      241 cggttgatgt ccagttcctg gtccacgtac atatcccgcc cattctgcag gtagtacagg
      301 tacagcttct cgttctgcag ctgggtgttt tccacggggt gttctttcag gatctggctg
      361 cccagctctt tgatgccctc ttcgatccgc ttcattctct cgcggctgtt cttctgtccc
      421 ttctgggtgg tctggttctc tctggccatt tcgatcacga tgttctcggg cttgtgccgg
      481 cccatcactt tcacgagctc gtccaccacc ttcactgtct gcaggatgcc cttcttaatg
      541 gcggggctgc cggccagatt ggcaatgtgc tcgtgcaggc tatcgccctg gccggacacc
      601 tgggctttct ggatgtcctc ttta
//
