LOCUS Exported 1079 bp ds-DNA linear SYN 27-MAY-2021 DEFINITION Express recepter TVA for envA with AAV. ACCESSION . VERSION . KEYWORDS pAAV-EF1a-flex-TVA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1079) AUTHORS Menegas W, Bergan JF, Ogawa SK, Isogai Y, Umadevi Venkataraju K, Osten P, Uchida N, Watabe-Uchida M TITLE Dopamine neurons projecting to the posterior striatum form an anatomically distinct subclass. JOURNAL Elife. 2015 Aug 31;4. doi: 10.7554/eLife.10032. PUBMED 26322384 REFERENCE 2 (bases 1 to 1079) AUTHORS . TITLE Direct Submission JOURNAL Exported May 27, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1079 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 50..83 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind 134..167 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." CDS 624..647 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" protein_bind complement(667..700) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(751..784) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." intron 824..1027 /label=EF-1-alpha intron A /note="truncated intron upstream of the start codon of human EF-1-alpha" primer_bind complement(847..867) /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 caattttgta atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta 61 tagcatacat tatacgaagt tattctttgc accattctaa agaataacag tgataatttc 121 tgggttaagg caaataactt cgtataggat actttatacg aagttatgct agagcttgat 181 gtggtggtac cgacatggcg cggctgctgc ccgcgctgct gctgctgctg ctgcccggta 241 acgtgaccgg taacgggtcc ggtaacggtt ctttgtcccg ttgccccccc ggtcagttcc 301 gctgctcgga gccgcccggt gcccacgggg agtgttaccc gcaggactgg ctgtgcgacg 361 gacaccccga ctgcgacgac gggcgggacg agtggggctg cgggaccagc gcgatccccg 421 cggtgcccac ggacaacggc acagaggctc ccactgcccc tgctcctgga cgtgctctgc 481 cagccaggaa tcacggccgc atgtggatgc tgatcactgc agtgctcctg tgctgcctgg 541 tagctgtggg tggtatcgct gcatggggga agtccaaagc aaaaagcagg tctgacatct 601 tcagtcttga aagcgcatcc aaggactaca aggacgacga cgacaagtag gatccactag 661 cgcgccataa cttcgtataa tgtatgctat acgaagttat taagaggttt catattgcta 721 atagcagcta caatccagct accattctgc ataacttcgt ataaagtatc ctatacgaag 781 ttattccgga gtcgactcta gaggatccgg tacctcacga cacctgaaat ggaagaaaaa 841 aactttgaac cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa 901 attccaagga gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag 961 tgtggggaaa ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa 1021 agctcgagaa ctaatcgagg tgcctggacg gcgcccggta ctccgtggag tcacatgaa //