LOCUS Exported 6977 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses human junctional adhesion molecule-A in mammalian cells. ACCESSION . VERSION . KEYWORDS hJAM-A pcDNA3.1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6977) AUTHORS Danthi P, Hansberger MW, Campbell JA, Forrest JC, Dermody TS TITLE JAM-A-independent, antibody-mediated uptake of reovirus into cells leads to apoptosis. JOURNAL J Virol. 2006 Feb;80(3):1261-70. PUBMED 16415003 REFERENCE 2 (bases 1 to 6977) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6977 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2571..2588) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2577..2801 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2847..3275 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2934..2953) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3144..3165 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3284..3304) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3289..3618 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3469..3604 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3531..3550 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3685..4479 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3739..3758) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4349..4368 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4653..4774 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4690..4709) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4744..4763 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4823..4839) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4823..4839) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4836..4858) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4847..4863 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4871..4901) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4916..4937 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5054..5071) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5225..5810) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5305..5324) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5981..6841) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6604..6623 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6842..6946) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccaaagaa ttcggcacga gtctcagtcc 961 cctcgctgta gtcgcggagc tgtgtctgtt cccaggagtc cttcggcggc tgttgtgtca 1021 gtggcctgat cgcgatgggg acaaaggcgc aagtcgagag gaaactgttg tgcctcttca 1081 tattggcgat cctgttgtgc tccctggcat tgggcagtgt tacagtgcac tcttctgaac 1141 ctgaagtcag aattcctgag aataatcctg tgaagttgtc ctgtgcctac tcgggctttt 1201 cttctccccg tgtggagtgg aagtttgacc aaggagacac caccagactc gtttgctata 1261 ataacaagat cacagcttcc tatgaggacc gggtgacctt cttgccaact ggtatcacct 1321 tcaagtccgt gacacgggaa gacactggga catacacttg tatggtctct gaggaaggcg 1381 gcaacagcta tggggaggtc aaggtcaagc tcatcgtgct tgtgcctcca tccaagccta 1441 cagttaacat cccctcctct gccaccattg ggaaccgggc agtgctgaca tgctcagaac 1501 aagatggttc cccaccttct gaatacacct ggttcaaaga tgggatagtg atgcctacga 1561 atcccaaaag cacccgtgcc ttcagcaact cttcctatgt cctgaatccc acaacaggag 1621 agctggtctt tgatcccctg tcagcctctg atactggaga atacagctgt gaggcacgga 1681 atgggtatgg gacacccatg acttcaaatg ctgtgcgcat ggaagctgtg gagcggaatg 1741 tgggggtcat cgtggcagcc gtccttgtaa ccctgattct cctgggaatc ttggtttttg 1801 gcatctggtt tgcctatagc cgaggccact ttgacagaac aaagaaaggg acttcgagta 1861 agaaggtgat ttacagccag cctagtgccc gaagtgaagg agaattcaaa cagacctcgt 1921 cattcctggt gtgagcctgg tcggctcacc gcctatcatc tgcatttgcc ttactcaggt 1981 gctaccggac tctggcccct gatgtctgta gtttcacagg atgccttatt tgtcttctac 2041 accccacagg gccccctact tcttcggatg tgtttttaat aatgtcagct atgtgcccca 2101 tcctccttca tgccctccct ccctttccta ccactgctga gtggcctgga acttgtttaa 2161 agtgtttatt ccccatttct ttgagggatc aggaaggaat cctgggtatg ccattgactt 2221 cccttctaag tagacagcaa aaatggcggg ggtcgcagga atctgcactc aactgcccac 2281 ctggctggca gggatctttg aataggtatc ttgagcttgg ttctgggctc tttccttgtg 2341 tactgacgac cagggccagc tgttctagag cgggaattag aggctagagc ggctgaaatg 2401 gttgtttggt gatgacactg gggtccttcc atctctgggg cccactctct tctgtcttcc 2461 catgggaagt gccactggga tccactagtc cagtgtggtg gaattctgca gatatccagc 2521 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt 2581 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 2641 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 2701 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 2761 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac 2821 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg 2881 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 2941 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 3001 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 3061 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 3121 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 3181 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 3241 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta 3301 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3361 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 3421 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 3481 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 3541 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga 3601 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa 3661 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 3721 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 3781 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 3841 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg 3901 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 3961 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 4021 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 4081 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 4141 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 4201 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 4261 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 4321 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 4381 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 4441 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 4501 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 4561 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 4621 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 4681 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 4741 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 4801 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 4861 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 4921 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 4981 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 5041 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 5101 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 5161 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 5221 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 5281 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 5341 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 5401 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 5461 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 5521 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 5581 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 5641 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 5701 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 5761 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 5821 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 5881 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 5941 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 6001 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 6061 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 6121 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 6181 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 6241 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 6301 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 6361 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 6421 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 6481 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 6541 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 6601 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 6661 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 6721 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 6781 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 6841 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 6901 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 6961 aagtgccacc tgacgtc //