LOCUS       Exported                9495 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  a targeting vector, to target the endogenous DAZL CPG island with 
            Snrp-GFP-polyA.
ACCESSION   .
VERSION     .
KEYWORDS    Dazl-CPG-TV
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Stelzer Y, Shivalila CS, Soldner F, Markoulaki S, Jaenisch R
  TITLE     Tracing Dynamic Changes of DNA Methylation at Single-Cell 
            Resolution.
  JOURNAL   Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
  PUBMED    26406378
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1752..2471
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1752..2468
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1797..1818)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2058..2077)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2405..2426
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2561..2580)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2626..2681
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2680..2699)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(3042..3075)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        3136..3632
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3171..3194)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3565..3584
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3672..4271
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3672..3691)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4168..4188
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(4828..4861)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        5827..5889
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     primer_bind     complement(5928..5947)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5929..5947)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5954..5971)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5954..5970)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5963..5985)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6111..6539
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6198..6217)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6408..6429
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             6883..7677
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6937..6956)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7547..7566
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             7695..8555
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7913..7932)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8726..9314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9215..9234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9468..9485
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 caggttatgc cctctcccca cttctctgtg ctacaccaga aaaggccgat ttttaaacct
      361 ccgcgtaagg aatcgcggcc tccggctggt cttgcactgc caccccgggg cctgccctcg
      421 actcaccatg atggcggcca gagttcgggt ggtaaaacct cgaaggtgga gcagaagcca
      481 cgcgacgggg cagctacgtg aggtggctgc ctagccggcc aacgggcaaa aaaaagccga
      541 ggcggtggtg gaaagagagc ccacggccac ggagacagcg gagtcccgaa gcgagcgtgc
      601 tgagggtcgc cgagtcactg aggcgtgggc tgcgcgcccc ggggggatga ctgtgcgtgg
      661 ctggcctcgt gacgtgctac agccaatagg cggcagcgct agggcctcgc aaggggggcc
      721 aggggaccca cgtcgcccac ggagtgcgtg aggggtgcgc atggagcatg cgcgacatct
      781 acgtaagggt ctcggctgcc gagcaggccc gagcctcact gacagacaga tggaccgact
      841 cctgccactc cctagccccc ccaggaccgt gtccgaagcc cctgtgaaat aagtcctggg
      901 caggacggtg ctcagagttc tgtcaggaaa gcatttattc aagtcattac ctcaaaaatg
      961 gcacctcgaa ggcatggcgt tatcccgtca gcaaagcttg gcggccgaat ttaaaattta
     1021 caaaatgccc gcagaaatag aaattaataa cgggcggcac gactagagag caggccttgc
     1081 ctggccgggc cccttcaccc tctttttcag ccacattgag aaggttgagt ccgggtgcat
     1141 gtggaaggcg cacctcatgc ctcacgggcc ggcccgtcag ccatcaggac ttagtttttt
     1201 tcttccgttt tttttctccc ccccccccgc cccttcaaat aaatgcccga attatccggt
     1261 acaaaggaca cagcagtgac cagaccacac gagagaatat ccctcccttc gtggaattta
     1321 ggttgcagta tgagtgtttc tcttgtaggc tttacagaaa caagttaggc cagctgagag
     1381 aattctagaa cattctcaaa gccagagaaa cggggcctac ctacctacag cagagccgag
     1441 ctgtagggtg cttggcaatt gacgctcaaa tttccgcagt aggaatgctc aagcattcct
     1501 tttggtagct gccttttggc aggacattcc ggtcagaggg acagagaccc ctgcattgcg
     1561 gcaaaaatgt gcgcatgtgc agccattgcc tgggacgcat gcgtagggag ccgcgcgaca
     1621 aacctgagcc attgcggcaa gactagcgca gagaggagag ggagccggag atgccagacg
     1681 cttggttctg aggagtgatt tgcaacgcaa tggagcgagg aaggtcagct gggcttgtgg
     1741 attctgctag catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1801 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1861 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1921 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     1981 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2041 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2101 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2161 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     2221 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     2281 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2341 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2401 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2461 tgtacaagta aacgcgtgaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     2521 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     2581 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     2641 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     2701 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     2761 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     2821 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     2881 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     2941 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     3001 ggagatccct taattaacga tcatattcaa taacccttaa tataacttcg tataatgtat
     3061 gctatacgaa gttattaggt ctgaagagga gtttacgtcc agccaagctt aggatctcga
     3121 cctcgaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     3181 tagcagcccc gctggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     3241 atccaccggt agcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     3301 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     3361 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     3421 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     3481 cagctgggaa gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     3541 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     3601 tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat ctcgagcagc
     3661 tgaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     3721 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     3781 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     3841 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     3901 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     3961 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     4021 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     4081 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     4141 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     4201 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     4261 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     4321 atgcatcgat gatatcagat ccccgggatg cagaaattga tgatctatta aacaataaag
     4381 atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg agaacagagt
     4441 acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg ggattagata
     4501 aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt tcatagttgg
     4561 atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc ccactcatga
     4621 tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc cactttgtgg
     4681 ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg agatcagcag
     4741 cctctgttcc acatacactt cattctcagt attgttttgc caagttctaa ttccatcaga
     4801 agctggtcga gatccggaac ccttaatata acttcgtata atgtatgcta tacgaagtta
     4861 ttaggtccct cgaagaggtt cactaggcgc gccagataac cttacggcag aaccacctag
     4921 tacttatacc gtcctgacta ccttttgtac agatgataag tattacagct tttttttttt
     4981 ttaaagctgg caaaacagct taagattgcc actaaccatg cagcaatgat ggagaaaata
     5041 gtattaaaat aataaataaa aataaaacaa ataagggtag attgcaccta tttgaaaggg
     5101 gggaaattcc aaaattctga aactaaaatt aagctcgaaa ctacagcggt gaagatccga
     5161 ttctccactg gccttcggta ggtggctaca tggtggtgtg aacagtctaa caaagggcct
     5221 ggactgtcct cattttagtg tagagaagaa aaaaacccac tacagacagc tggaatggtt
     5281 ggttaatgag cagcggagag atggctctaa actctgtagc tccaggcctt cctgcgttgg
     5341 ctggatgggg aaagctgaag cggtgcagcc cgatttggag ctgagcacct tgcagcacac
     5401 cggtgttctg gcaaggcatc atcctgagga aaagcacgga cgtgagggga cggggagcac
     5461 cgtgcagact agagcaaacc caaaaccatc taactctcat tggtgtttga ccttccattc
     5521 ctgcgctctc caagtttggc gggccggcca ataagggcga attctgcaga tatccatcac
     5581 actggcggcc gcggaccgta gagtcgagca gtgtggtttt caagaggaag caaaaagcct
     5641 ctccacccag gcctggaatg tttccaccca atgtcgagca gtgtggtttt gcaagaggaa
     5701 gcaaaaagcc tctccaccca ggcctggaat gtttccaccc aatgtcgagc aaaccccgcc
     5761 cagcgtcttg tcattggcga attggaacac gcagatgcag tcggggcggc gcggtcccag
     5821 gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga
     5881 cccgcttaac gcggccgctc gagcatgcat ctagagggcc caattcgccc tatagtgagt
     5941 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6001 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6061 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
     6121 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6181 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6241 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6301 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6361 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6421 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6481 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6541 taacaaaatt cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg
     6601 cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac
     6661 gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg
     6721 gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
     6781 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     6841 ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6901 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6961 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     7021 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     7081 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     7141 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatcccac
     7201 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     7261 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     7321 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     7381 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     7441 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     7501 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     7561 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     7621 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt
     7681 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7741 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7801 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7861 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7921 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7981 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8041 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8101 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8161 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8221 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8281 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8341 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8401 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8461 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8521 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8581 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8641 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9421 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9481 gcgaggaagc ggaag
//
