LOCUS Exported 10296 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Second generation Rac1 FRET biosensor for lentivirus production. ACCESSION . VERSION . KEYWORDS pLenti-Rac1-2G SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10296) AUTHORS Fritz RD, Menshykau D, Martin K, Reimann A, Pontelli V, Pertz O TITLE SrGAP2-Dependent Integration of Membrane Geometry and Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of Locomotion. JOURNAL Dev Cell. 2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 10.1016/j.devcel.2015.09.002. PUBMED 26439400 REFERENCE 2 (bases 1 to 10296) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10296 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 26..130 /gene="bla" /label=AmpR promoter CDS 131..991 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(349..368) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1162..1750 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1651..1670 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1904..1921 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2038..2059 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2074..2104 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2112..2128 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2117..2139 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2136..2152 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2136..2152 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2171..2191 /label=T3 /note="T3 promoter, forward primer" promoter 2173..2191 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2447..2627 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2671..2796 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3289..3522 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3707..3751 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4012..4129 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind complement(4135..4155) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4140..4469 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4320..4455 /label=SV40 ori /note="SV40 origin of replication" CDS 4479..5078 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" primer_bind complement(4479..4498) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4975..4995 /label=Puro-F /note="Puromycin resistance gene, forward primer" enhancer 5150..5453 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5454..5657 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 5608..5628 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 5654..5678 /label=LNCX /note="Human CMV promoter, forward primer" CDS 5761..5778 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 5788..5817 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 7063..7581 /codon_start=1 /product="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN173 /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIE" primer_bind complement(7108..7129) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7369..7388) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7716..7737 /label=EGFP-C /note="EGFP, forward primer" CDS 7756..8334 /codon_start=1 /product="human Rac family small GTPase 1" /label=Rac1 /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR GLKTVFDEAIRAVLCPPPVKKRKRKCLLL" misc_feature 8358..8946 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8411..8431) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8829..8840) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9037..9270 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 9342..9463 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9379..9398) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9433..9452 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 9482..9617 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(9649..9668) /label=T7 /note="T7 promoter, forward primer" promoter complement(9650..9668) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9678..9695) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9678..9694) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9687..9709) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9839..10294 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9926..9945) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10136..10157 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg 2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt 2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg 2401 cattgcagag atattgtatt taagtgccta gctcgataca ataaacgggt ctctctggtt 2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac 2641 agggacctga aagcgaaagg gaaaccagag ctctctcgac gcaggactcg gcttgctgaa 2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc 2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga 2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat 2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca 2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa 3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag 3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc 3121 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg 3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac 3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt 3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcctcaatga cgctgacggt 3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat 3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag 3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc 3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct 3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta ttgtggaatg 4141 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 4201 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 4261 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 4321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 4381 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 4441 gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg 4501 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 4561 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 4621 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 4681 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 4741 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 4801 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 4861 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 4921 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 4981 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc 5041 gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc 5101 gcccgaccga aaggagcgca cgacccaatc gataagcttg ggagttccgc gttacataac 5161 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 5221 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 5281 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 5341 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 5401 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 5461 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 5521 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 5581 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 5641 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct 5701 gttttgacct ccatagaaga caccgactct agatacaatc aaaggagata taccatggca 5761 caccatcacc accatcacgg gagcggggag cagaaactaa tatcagagga ggatcttggc 5821 tcaggatcct ccaccgacgg catggacgag ctgtacaagg gtgctagcgg aggaatggtg 5881 agcaagggcg aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag 5941 atggagggca acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc 6001 tacgacggca ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc 6061 tacgacattc tgaccaccgc gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac 6121 gacatcccca actacttcaa gcagtccttc cccgagggct actcttggga gcgcaccatg 6181 accttcgagg acaagggcat cgtgaaggtg aagtccgaca tctccatgga ggaggactcc 6241 ttcatctacg agatacacct caagggcgag aacttccccc ccaacggccc cgtgatgcag 6301 aagaagacca ccggctggga cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg 6361 aagggcgacg tcaagcacaa gctgctgctg gagggcggcg gccaccaccg cgttgacttc 6421 aagaccatct acagggccaa gaaggcggtg aagctgcccg actatcactt tgtggaccac 6481 cgcatcgaga tcctgaacca cgacaaggac tacaacaagg tgaccgttta cgagagcgcc 6541 gtggcccgca actccggaaa agagaaagag cggccagaga tttctctccc ttcagatttt 6601 gaacacacaa ttcatgtcgg ttttgatgct gtcacagggg agtttacggg aatgccagag 6661 cagtgggccc gcttgcttca gacatcaaat atcactaagt cggagcagaa gaaaaacccg 6721 caggctgttc tggatgtgtt ggagttttac aactcgaaga agacatccaa cagccagaaa 6781 tacatgagct ttacagataa gtcagctccc gggacttctg gttctggtaa acctggttct 6841 ggtgaaggtt ctactaaagg gacttctggt tctggtaaac ctggttctgg tgaaggttct 6901 actaaaggga cttctggttc tggtaaacct ggttctggtg aaggttctac taaagggact 6961 tctggttctg gtaaacctgg ttctggtgaa ggttctacta aaggggcggc cgccatgatc 7021 actctcggca tggacgagct gtacaagggt ggcagcggtg gcatggtgag caagggcgag 7081 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 7141 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 7201 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc 7261 tacggcctga tgtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag 7321 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 7381 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 7441 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 7501 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc 7561 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac 7621 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc 7681 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 7741 gccgccgggg gtaccatgca ggccatcaag tgtgtggtgg tgggagacgg agctgtaggt 7801 aaaacttgcc tactgatcag ttacacaacc aatgcatttc ctggagaata tatccctact 7861 gtctttgaca attattctgc caatgttatg gtagatggaa aaccggtgaa tctgggctta 7921 tgggatacag ctggacaaga agattatgac agattacgcc ccctatccta tccgcaaaca 7981 gatgtgttct taatttgctt ttcccttgtg agtcctgcat catttgaaaa tgtccgtgca 8041 aagtggtatc ctgaggtgcg gcaccactgt cccaacactc ccatcatcct agtgggaact 8101 aaacttgatc ttagggatga taaagacacg atcgagaaac tgaaggagaa gaagctgact 8161 cccatcacct atccgcaggg tctagctatg gccaaggaga ttggtgctgt aaaatacctg 8221 gagtgctcgg cgctcacaca gcgaggcctc aagacagtgt ttgacgaagc gatccgagca 8281 gtcctctgcc cgcctcccgt gaagaagagg aagagaaaat gcctgctgtt gtgactcgag 8341 caccagaatt cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 8401 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 8461 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 8521 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 8581 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 8641 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 8701 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 8761 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 8821 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 8881 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 8941 ccccgcctgg aattaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg 9001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 9061 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 9121 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 9181 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 9241 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 9301 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 9361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 9421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 9481 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 9541 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 9601 aggctttttt ggaggcctag gcttttgcgt cgagacgtac ccaattcgcc ctatagtgag 9661 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 9721 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 9781 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac 9841 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 9901 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 9961 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 10021 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 10081 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 10141 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 10201 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 10261 gcgaatttta acaaaatatt aacgtttaca atttcc //