LOCUS       Exported                 962 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Expresses APEX2 in the cytosol of mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3 APEX2-NES
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(47..427)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(109..130)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     321..340
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    473..697
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     686..703
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        723..741
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     723..740
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(778..804)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
       61 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
      121 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
      181 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
      241 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
      301 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
      361 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
      421 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
      481 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
      541 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
      601 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
      661 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgagctcta
      721 gcatttaggt gacactatag aatagggccc tctagatgca tgctcgagct attagtccag
      781 ggtcaggcgc tccaggggag gcagctgcag ggcatcagca aacccaagct cggaaagctt
      841 ttggtgagcc tcagcgtaat cagcaaagaa ggcatcttcg tccgctgcat atttgtcaac
      901 gagagggcgg aatacagggt cagacaaaag agccttgtca gaaggtagct gaaggagacc
      961 tt
//
