LOCUS       Exported                 796 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  pJL1 expression vector with sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    pJL1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 796)
  TITLE     pJL1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             639..686
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             687..710
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(765..783)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gtgaagaact gtttaccggc gttgtgccga ttctggtgga actggatggc gatgtgaacg
       61 gtcacaaatt cagcgtgcgt ggtgaaggtg aaggcgatgc cacgattggc aaactgacgc
      121 tgaaatttat ctgcaccacc ggcaaactgc cggtgccgtg gccgacgctg gtgaccaccc
      181 tgacctatgg cgttcagtgt tttagtcgct atccggatca catgaaacgt cacgatttct
      241 ttaaatctgc aatgccggaa ggctatgtgc aggaacgtac gattagcttt aaagatgatg
      301 gcaaatataa aacgcgcgcc gttgtgaaat ttgaaggcga taccctggtg aaccgcattg
      361 aactgaaagg cacggatttt aaagaagatg gcaatatcct gggccataaa ctggaataca
      421 actttaatag ccataatgtt tatattacgg cggataaaca gaaaaatggc atcaaagcga
      481 attttaccgt tcgccataac gttgaagatg gcagtgtgca gctggcagat cattatcagc
      541 agaatacccc gattggtgat ggtccggtgc tgctgccgga taatcattat ctgagcacgc
      601 agaccgttct gtctaaagat ccgaacgaaa aaggcacgcg ggaccacatg gttctgcacg
      661 aatatgtgaa tgcggcaggt attacgtgga gccatccgca gttcgaaaaa taagtcgacc
      721 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      781 agcataaccc cttggg
//
