LOCUS       Exported                1060 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  pJL1 expression vector with sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    pJL1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1060)
  TITLE     pJL1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      97..144
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     140..158
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(213..236)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(237..284)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     RBS             940..962
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(993..1012)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(994..1012)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
       61 gctcgatgag tttttctaat cagaattggc tttcagcaaa aaacccctca agacccgttt
      121 agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcca actcagcttc
      181 ctttcgggct ttgttagcag ccggtcgact tatttttcga actgcggatg gctccacgta
      241 atacctgccg cattcacata ttcgtgcaga accatgtggt cccgcgtgcc tttttcgttc
      301 ggatctttag acagaacggt ctgcgtgctc agataatgat tatccggcag cagcaccgga
      361 ccatcaccaa tcggggtatt ctgctgataa tgatctgcca gctgcacact gccatcttca
      421 acgttatggc gaacggtaaa attcgctttg atgccatttt tctgtttatc cgccgtaata
      481 taaacattat ggctattaaa gttgtattcc agtttatggc ccaggatatt gccatcttct
      541 ttaaaatccg tgcctttcag ttcaatgcgg ttcaccaggg tatcgccttc aaatttcaca
      601 acggcgcgcg ttttatattt gccatcatct ttaaagctaa tcgtacgttc ctgcacatag
      661 ccttccggca ttgcagattt aaagaaatcg tgacgtttca tgtgatccgg atagcgacta
      721 aaacactgaa cgccataggt cagggtggtc accagcgtcg gccacggcac cggcagtttg
      781 ccggtggtgc agataaattt cagcgtcagt ttgccaatcg tggcatcgcc ttcaccttca
      841 ccacgcacgc tgaatttgtg accgttcaca tcgccatcca gttccaccag aatcggcaca
      901 acgccggtaa acagttcttc acctttgctc atatgtatat ctccttctta aagttaaaca
      961 aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat taatttcgcg
     1021 ggatcgcgtt gctggcgttt ttccataggc tccgcccccc
//
