LOCUS Exported 3895 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pHsp18.2 (GB0153) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3895) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3895) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3895 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(945..963) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(946..963) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(981..997) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(981..997) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(994..1016) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1005..1021 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1029..1059) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1074..1095 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1212..1229) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1383..1971) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1463..1482) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2142..3002) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2765..2784 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3003..3107) /gene="bla" /label=AmpR promoter rep_origin complement(3185..3640) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3322..3343) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3534..3553 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3766..3788 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3780..3797 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3781..3797 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3804..3823 /label=T7 /note="T7 promoter, forward primer" promoter 3804..3822 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3853..3862 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3857..3866 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgcattga cttttggttc ttgattttcc tgctgatttg atctgattag caaaggacat 61 atttataggg aagttagagg atgaagagag aatgttcttc gagtttcttg aatagaatag 121 aaaatgctcc tttttctaaa accttcgctt ggagtcttta gaacacatac acaatgatca 181 ctgtggtgaa atgaccagat ttttcttggc atttcaggaa gtttcgtttg ttgaaacaag 241 accgaaatgc aatccttggt tacaattaaa actacgtgcc gttttgcttt gttgttgtat 301 tatcctcttc gagatacggg ctcagtgaag cggatcttct ttgcaaaagc ccatttctta 361 ataggtcgga agtggaattt aagaaaatct aactcagaga aaagaggaca aaatcaagat 421 ttaagattat tagcttttcg ccaaaggaaa actatagtaa gttctcgcaa taacagttgg 481 cactaaattt ccgagaatgt caacaatctt ttaatggatt tttttgtctc catatataca 541 agaactgagc cacgtttttg tgacaaacat agacaacata tttactaaac aagtaaacta 601 aaaaaaaaaa aaatctaaat gattcgattc aagtatcagt tactgtgatc aaaatctcaa 661 cttatttatt gcctgttcat gtcatgcttg aaccagaaga aatgaccatg gaaagtaatt 721 gtgattacct ttttgggaaa cttggggtta aaaattgaaa atgtgatatg tcatggtttt 781 ggtttctaat caatagaact tgcatgaaag aaagaaagaa agagaaatga agagaaaagc 841 ttctcccgag accactcatc gcccaatcac tagtgaattc gcggccgcct gcaggtcgac 901 catatgggag agctcccaac gcgttggatg catagcttga gtattctata gtgtcaccta 961 aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 1021 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 1081 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 1141 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 1201 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 1261 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 1321 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 1381 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 1441 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 1501 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 1561 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 1621 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 1681 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 1741 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 1801 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 1861 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 1921 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 1981 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 2041 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 2101 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 2161 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 2221 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 2281 cgcgacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 2341 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 2401 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 2461 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 2521 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 2581 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 2641 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 2701 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 2761 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 2821 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 2881 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 2941 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 3001 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 3061 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 3121 aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 3181 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 3241 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 3301 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 3361 tccaacgtca aagggcgaaa aaccgtctat cagggcgctg gcccactacg tgaaccatca 3421 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 3481 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 3541 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 3601 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc 3661 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 3721 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 3781 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt 3841 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tgggcgatga gtggt //