LOCUS Exported 5038 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pHsp70B (GB0155) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5038) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 5038) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5038 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(2088..2106) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(2089..2106) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2124..2140) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2124..2140) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2137..2159) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 2148..2164 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2172..2202) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2217..2238 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2355..2372) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2526..3114) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2606..2625) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3285..4145) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3908..3927 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4146..4250) /gene="bla" /label=AmpR promoter rep_origin complement(4328..4783) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4465..4486) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4677..4696 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4909..4931 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4923..4940 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4924..4940 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 4947..4966 /label=T7 /note="T7 promoter, forward primer" promoter 4947..4965 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 4996..5005 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 5000..5009 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgcatttg ctaaaaaaaa gcttcagtaa ttgaatgaaa ttggaagtga ggtttttcga 61 tttcttttgt ttgtttatct gtttgatgaa gaatcggaaa cgatggaaga agctatatat 121 acgaatccga cgagaggaac gagacatttc gagaaaaaag gtcgtggcac aacccagggg 181 attgtggaaa ggtggagaaa tcgcgcaaac gtacaagaga gttctggatt caccgaccgg 241 gatatatttt atctccggcg accgcttgat aattaaatgg gcctattaaa gcccatatgg 301 tataaacttg taggcccgta tatagaataa aaggcccagt gtaagttctg gaggattttc 361 aatattttgt ttacgtcttc tttcttcact tgttctgtga tttcgaagac gagaaaggaa 421 aagccatgaa agaggcgaag gagaagaaga atccagaaat ctccgttagt atcacgatat 481 caacttgggg ggcttttgct gtttatgtga ttactgggtt atttctgatc ttcggatttc 541 acgtggtccg taacaaatac tctgtcgatc tcatttctga tccaactctc actctgcgtc 601 tgctatgggt actaaaactt tctttcataa cctggttgct agtggatatt tttcagttaa 661 ttttagcctt tttgttttga tgttgatatt cagattattg agttcccgat cgtggtaatc 721 atctacagct tgttccgacg aaacccagag aaatgctcgg tgagtatagt tcatctctga 781 ggttctctgt gagaattaaa gaaactgaat ttctcgtttt aactcgattg ctttgtgctt 841 gtctcatttc ccttttatga aattgacaaa acaagctagt atcctcactt tacactctta 901 atgctcaaaa aaatcgtttc ttttcatata gtattatttt ggtcttgtgt gttttgactc 961 tgtattcatg attttctcat tgatttccag tactttagag ctgttggccg aagcttagtc 1021 ggactaattg ccggttagta tttctgtttt tcagcttatt atagctcact aatttttcaa 1081 agaagttgat tgctcaatga gttcacctgg aaattctaga tgttaagcta aacctgaaga 1141 tgtgagtgag tctgagcttc ttatgctttc atcttgtgct tttgctgatt cagtatccct 1201 tgattgtgtc tactatatct atacctgtgt tttgatttgt ttaataggtg ctcttataaa 1261 tgctttggga gctgtttctt tgggtgcacc tattggaatg cagtatgttt cactaagatc 1321 ttctttctct atatcttttt atttctttct aattgtggaa atgttgattg taaatctgga 1381 cttttcagtt gtccagatta aaaatgaggc tccttaatgc tggttgtggt ctgacttctg 1441 catggagatt gtgtgatttt caaaagctga catgagtatt accttctcat cttatgcaga 1501 tctctatcaa aaacaattca ctggtccttt ctaatgtctg ttttcacggt aagctgcagt 1561 gtccatgact taaaagatgt taataccatg ccatatgtgt atttctgcag tttgaaacaa 1621 accttttgat ggtgtctgat gattttgatt ttcataggtt gtaccagcaa ctgcagtttt 1681 aggtgcatca tggatagatt ggcatcgtat ctttgcttca ctaaagtaag tcacttatgc 1741 tacctagtat gataattcac acactttatg ttgttgttaa tccatctttt ttttttcttt 1801 tagaccaatc ggaattatag aacatatgct cttagttcca gcatatggag caattattgg 1861 aggatggttt ggagcttggc ctatgccact tgactgggaa agaccatggc aggtaatgtt 1921 tatttcttct caacatacag tcaagggctt gtttctgaac tttattgctc ccttttttcg 1981 cagttctccc gagaccactc atcgcccaat cactagtgaa ttcgcggccg cctgcaggtc 2041 gaccatatgg gagagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac 2101 ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 2161 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 2221 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 2281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 2341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 2401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 2461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 2521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 2581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 2641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 2701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 2761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 2821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 2881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 2941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 3001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 3061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 3121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 3181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 3241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 3301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 3361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 3421 ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 3481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 3541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 3601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 3661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 3721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 3781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 3841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 3901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 3961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 4021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 4081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 4141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 4201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 4261 cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 4321 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc 4381 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag 4441 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg 4501 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact acgtgaacca 4561 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa 4621 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg 4681 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta 4741 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc 4801 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 4861 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 4921 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggcccga 4981 cgtcgcatgc tcccggccgc catggcggcc gcgggaattc gattgggcga tgagtggt //