LOCUS Exported 3847 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pPAct2noUTR (GB0192) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3847) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3847) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3847 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(898..916) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(899..916) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(934..950) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(934..950) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(947..969) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 958..974 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(982..1012) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1027..1048 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1165..1182) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1336..1924) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1416..1435) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2095..2955) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2718..2737 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2956..3060) /gene="bla" /label=AmpR promoter rep_origin complement(3138..3593) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3275..3296) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3487..3506 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3719..3741 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3733..3750 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3734..3750 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3757..3776 /label=T7 /note="T7 promoter, forward primer" promoter 3757..3775 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3806..3815 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3810..3819 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgagtatt caaagcggag aggaaaatat atgaatttat ataggcgggt ttatctctta 61 caactttatt ttcggccttt caaaaaaata attaaaatcg acagacacga atcatttcga 121 ccacaggtaa agataacgtg acctggctgt cagacagcct tttccctcgt gttaactaat 181 ttttaaacta attaatcatc tcagcccttg gattagttct tttgctttga tggcttcatg 241 actgtgacct gctcgatccg cgtgttacat gacagctccg tttttttagt ggttaactta 301 aaccgagtca atccaggcaa cgttagtcgt cgtcgtggtt ggcttgttca attagatttc 361 atacaattca acgtaattta attcgttttc tattagaatt gtatcataat taattcagac 421 cgtgaaagaa agtgtctttc atgatgtgtt tatggatatt tatacaataa gatacaatgt 481 ttcatcatat tcactattca cgattagtat gtacattaaa taatggctac tactacatcc 541 gaactcgtca aaacgattct gaatcaatta tacatatgct gactcttgca tacataaaaa 601 atagttgttt aaattttgtc taactaatgt ttggtataag tataatgttg agttgagata 661 ccaattacat cgagtctagc cattttgtcg tgccatattc gtcaaaactt tcttacataa 721 tgataaccta gatctagatg agatatgtat caatgtattt gagatcataa ttaagttcgt 781 tctaaatttt gtcgactccc gagaccactc atcgcccaat cactagtgaa ttcgcggccg 841 cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct tgagtattct 901 atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 961 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 1021 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 1081 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 1141 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 1201 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 1261 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 1321 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 1381 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 1441 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 1501 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 1561 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 1621 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 1681 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 1741 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 1801 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 1861 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 1921 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 1981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 2041 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 2101 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 2161 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 2221 tgcaatgata ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 2281 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 2341 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 2401 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 2461 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 2521 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 2581 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 2641 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 2701 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 2761 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 2821 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 2881 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 2941 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 3001 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 3061 cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga 3121 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt 3181 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 3241 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 3301 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact 3361 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 3421 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 3481 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 3541 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg 3601 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 3661 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 3721 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa 3781 ttgggcccga cgtcgcatgc tcccggccgc catggcggcc gcgggaattc gatgggcgat 3841 gagtggt //