LOCUS Exported 3070 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION General Purpose cloning vector for ""INT""-like constructs under U6-promoter expression.. ACCESSION . VERSION . KEYWORDS pU6_(Gluc)_INT(GenPurpClon) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3070) AUTHORS Shechner DM, Hacisuleyman E, Younger ST, Rinn JL TITLE Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. JOURNAL Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. PUBMED 26030444 REFERENCE 2 (bases 1 to 3070) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3070 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(1..861) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 624..643 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(862..966) /gene="bla" /label=AmpR promoter primer_bind 1034..1052 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(1090..1112) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 1212..1231 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1425..1447 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1439..1456 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1440..1456 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 1620..1639 /label=LKO.1 5' /note="Human U6 promoter, forward primer" primer_bind complement(1910..1926) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1910..1926) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1923..1945) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1934..1950 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1958..1988) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2003..2024 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2141..2158) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2312..2900) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2392..2411) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 1021 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 1081 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 1141 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 1201 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 1261 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 1321 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 1381 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 1441 taaaacgacg gccagtgttt cccatgattc cttcatattt gcatatacga tacaaggctg 1501 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt 1561 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg 1621 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt 1681 ggaaaggacg aaacaccgat ctagatacga ctcactatgt ttaagagcta tgctgcgaat 1741 acgagaagtc ttcttttttg aagacaatcg tattcgcagc atagcaagtt taaataaggc 1801 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct taattaagtc 1861 tagagtcgac tgtttaaacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt 1921 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 1981 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 2041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 2101 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 2161 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 2221 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 2281 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 2341 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 2401 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 2461 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 2521 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 2581 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 2641 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 2701 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 2761 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 2821 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 2881 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 2941 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 3001 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 3061 ggtctgacag //